RedTaq (Taq-polymerase labeled with red pigment) 250 units - M33

Package Size: 250 units Red-Taq DNA polymerase is a mixture of Genaxxon DNA polymerase and red pigment. After addition of Red-Taq to the PCR reaction mixture a thin red layer on the bottom of the tube appears. So you have a visible pipetting control. Furthermore you can see, if your reaction is already mixed, when the colour of your reaction becomes uniform. An aliquot of the samples (5-10 ?L) can then be loaded directly onto an agarose gel for electrophoresis following PCR. The red dye migrates slightly faster than bromophenol blue at about the same rate as a 125 base pair fragment in a 1% agarose gel. Since no additional loading buffers are added to the reaction following PCR, reamplification is possible. The red dye has no effect on automated or manual sequencing, restriction digestions or other downstream applications. However, if removing the dye is desired, this can easily be accomplished using any standard purification method.

RedTaq (Taq-polymerase labeled with red pigment) Product Details Document (pdf)
RedTaq (Taq-polymerase labeled with red pigment) Productdetails (pdf)
RedTaq (Taq-polymerase labeled with red pigment) Manual / Protocol (pdf)
RedTaq (Taq-polymerase labeled with red pigment) Manual / Certificate (pdf)

Source: Thermophilus aquaticus

Technical Data: 1 U/µL

Shipping / Storing Information: shipped on blue-ice, store at -20°C

Unit Definition: One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.

Productnumber: M3305.0250

changedate: 12.5.2011

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