ProbeMasterMix (2X) High ROX 2.5 mL - M3010.0100

Package Size: 2.5 mL The Genaxxon ProbeMastermix is shipped in aliquots of 2 x 1.25mL (equals 200 x 25µL reactions/250x20µL reactions). The mastermix is optimised for Real-Time PCR assays in Block systems and capillaries and contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains optimised amounts of all ingredients (Hot-Start Polymerase, dATP, dCTP, dGTP, dUTP, ROX (500nM) as internal standard, optimized reaction buffer with KCl and MgCl2, stabilizers and enhancers) to enable even amplification of low copy number targets. Data show that this kit can perform comparable to ones from market leaders. The small aliquote size of 1.25mL makes handling and storage easier (less freeze - thaw cycles per aliquot). The mix contains also dUTP instead of dTTP to have the possibility to prevent carry-over contaminations of DNA from previous PCR reactions. HighROX ProbeMastermix is specially suited for the following instruments: ABI7000, 7300, 7700, 7900; StepOnePlus(TM), StepOne(TM); Eppendorf Realplex4.

ProbeMasterMix (2X) High ROX Product Details Document (pdf)
ProbeMasterMix (2X) High ROX Manual / Protocol (pdf)

Application: PCR Primer extension Multiplex PCR Low-copy targets PCR Real-time PCR Quantitative PCR

Features: High Sensitivity and Specifity, Optimised buffer system, Reproducibility over a wide range of template amounts, Broad dynamic range.

Technical Data: The GreenMastermix is a ready-to-use 2X mastermix that contains dUTP instead of dTTP, ROX (500nM) as passive Reference dye, EvaGreen as fluorescent dye.

Shipping / Storing Information: shipped on blue-ice, store at -20°C

Productnumber: M3010.0100

changedate: 14.4.2011

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