SuperHot Mastermix (2-times) 12.5 mL - M3007.0500

Package Size: 12.5 mL Mix for 1000 x 25µL reactions (12.5mL). The Genaxxon 2X Hotstart Master Mix is a premixed, ready-to-use solution containing Hotstart Taq DNA Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR (one tube, one pipetting step). Just add your primers and template DNA. The PCR Master Mix has been optimized for use in routine and diagnostic PCR for amplifying DNA template in the range of 0.2-4kb. The special formulation of our Master Mix guarantees you reproducible results even after repeated freezing/thawing steps leading to good yields and sensitivity. The kit is efficient (no left over of reagents), scalable from 10µL to 50µL and stable for 12 months.

SuperHot Mastermix (2-times) Product Details Document (pdf)
SuperHot Mastermix (2-times) Productdetails (pdf)
SuperHot Mastermix (2-times) Manual / Protocol (pdf)

Application: PCR master mix for highly convenient PCR

Features: fast, sensitive, convenient

Shipping / Storing Information: shipped on blue-ice, store at -20°C

Unit Definition: One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.

Productnumber: M3007.0500

changedate: 20.5.2008

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