Proteasome
Antiserum to NEDD8 - Antiserum to Ubc9 - Fraction I (Frl. Hela) - Fraction II (FII from Hela cells) - Fraction II (FII from rabbit reticulocytes) - mammalian 20S Proteasome - Mammalian 26S-Proteasome Fraction - methylated Ubiquitin, min. 95% - PA28 Activator, min. 95.0% - Proteasome Substrate I (fluorogenic), min. 95% - Proteasome Substrate II (fluorogenic), min. 95% - Proteasome Substrate III (fluorogenic), min. 95% - recom. Mammalian (HIS)6 Ubiquitin
Antiserum to NEDD8 200 µL - S5263.0200
567,83 EUR
Package Size: 200 µL
From Rabbit. Specificity: Recognizes human, mouse and rat NEDD8. Does not cross react with ubiquitin or SUMO-1/sentrin-1. Application: WB, ICC, IHC.
Source: rabbit
Technical Data: liquid antiserum from rabbit; protect from moisture. Highly hygroscopic.
Shipping / Storing Information: shipped at …
Antiserum to Ubc9 50 µg - S5264.0050
504,73 EUR
Package Size: 50 µg
From sheep. Liquid. Antigen: C-terminal peptide from human Ubc9. Specificity: Recognizes human Ubc9. Application: WB IF.
Source: sheep
Technical Data: purified liquid antiserum against human Ubc9; dissolved in 50 mM Tris HCL, pH 7.5, 100 mM NaCl, 19% BSA, 10% Glycerol.
Shipping / Storing …
Fraction I (Frl. Hela) 2.5 mg - S5309.0003
559,94 EUR
Package Size: 2.5 mg
Contains ubiquitin, FI E2s, and FI E3s. Ideal for demonstrating if FI enzymes are critical for degradation of in vitro substrates as well as in vitro conjµgation activity.
Source: Hela S3 cells
Technical Data: Formulation: 7 mg/mL in 25 mM HEPES, pH7.6.
Shipping / Storing Information: shipped on …
Fraction II (FII from Hela cells) 2.5 mg - S5310.0003
520,51 EUR
Package Size: 2.5 mg
Ideal for demonstrating ubiquitin and/or ATP dependent degradation of in vitro substrates as well as in vitro conjµgation activity in the presence of proteasome inhibitor and Ub-H.
Source: Hela S3 cells
Technical Data: 8 mg/mL in 25 mM HEPES, pH7.6.
Shipping / Storing Information: shipped at RT, …
Fraction II (FII from rabbit reticulocytes) 2.5 mg - S5311.0003
520,51 EUR
Package Size: 2.5 mg
Ideal for demonstrating ubiquitin and/or ATP- dependent degradation of in vitro substrates as well as in vitro conjµgation activity in the presence of proteasome inhibitor and isopeptidase inhibitor.
Source: rabbit reticulocytes
Technical Data: 8 mg/mL in 25 mM HEPES, pH7.6.
Shipping / Storing …
mammalian 20S Proteasome 25 ug - S5261.0025
473,19 EUR
Package Size: 25 ug
Known to hydrolyze various peptide substrates and proteins with broad specificity in a non-ATP-dependent process.
Source: mammalian
Technical Data: min. 95%; MW = 110 kDa; dissolved in 0.4 mg/mL (3.6 uM) in 50 mM HEPES, pH7.6.
Shipping / Storing Information: shipped on dry-ice, store at -80°C …
Mammalian 26S-Proteasome Fraction 200 µL - S5262.0200
567,83 EUR
Package Size: 200 µL
Preferred for the degradation of pre-formed ubiquitin-protein conjµgates by the 26S proteasome since highly purified 26S is costly and more labile.
Source: mammalian
Technical Data: min. 65%; dissolved at 1.4 mg/mL in 50 mM HEPES, pH7.6; 0.5 mM MgATP, 150 mM NaCl, 10% glycerol.
Shipping / Storing …
methylated Ubiquitin, min. 95% 1 mg - S5314.0001
134,07 EUR
Package Size: 1 mg
Ideal for the reduction in polyUb chain length as well as conjµgation rates. Synthesised by reductive methylation of amines resulting in ubiquitin unable to form polyUb chains via lysine linkages with other Ub molecules.
Source: synthetic
Technical Data: lyophilised powder, to be reconstituted in …
methylated Ubiquitin, min. 95% 5 mg - S5314.0005
444,80 EUR
Package Size: 5 mg
Ideal for the reduction in polyUb chain length as well as conjµgation rates. Synthesised by reductive methylation of amines resulting in ubiquitin unable to form polyUb chains via lysine linkages with other Ub molecules.
Source: synthetic
Technical Data: lyophilised powder, to be reconstituted in …
PA28 Activator, min. 95.0% 25 µg - S5317.0025
429,02 EUR
Package Size: 25 µg
Enhances the 20S proteasome hydrolysis of peptide substrates (peptidase activity) but not hydrolysis of ubiquinated proteins. Composed of both alpha and beta subunits. Alternative to SDS activation. Lee, D.H. and Goldberg A.L., Selective Inhibitors of the Proteasome-Dependent and Vacuolar Pathways of …
Proteasome Substrate I (fluorogenic), min. 95% 5 mg - S5332.0005
271,29 EUR
Package Size: 5 mg
Fluorogenic proteasome substrate. Good for measuring the peptidase activity of the 20S proteasome.
Source: synthetic
Technical Data: min. 95.0%; Z-LLL-AMC.
Shipping / Storing Information: shipped at RT, stored at -20°C.
Productnumber: S5332.0005
changedate: 6.3.2002
Proteasome Substrate I (fluorogenic), min. 95% 5 x 5 mg - S5332.
1.175,08 EUR
Package Size: 5 x 5 mg
Fluorogenic proteasome substrate. Good for measuring the peptidase activity of the 20S proteasome.
Source: synthetic
Technical Data: min. 95.0%; Z-LLL-AMC.
Shipping / Storing Information: shipped at RT, stored at -20°C.
Productnumber: S5332.0025
changedate: 6.3.2002
Proteasome Substrate II (fluorogenic), min. 95% 5 mg - S5333.000
271,29 EUR
Package Size: 5 mg
Fluorogenic proteasome substrate. Good for measuring the peptidylglutamyl peptide-hydrolyzing activity of the 20S proteasome.
Source: synthetic
Technical Data: min. 95,0%, Z-LLE-AMC.
Shipping / Storing Information: shipped at RT, stored at -20°C.
Productnumber: S5333.0001
changedate: 6.3.2002
Proteasome Substrate II (fluorogenic), min. 95% 5 x 5 mg - S5333
1.175,08 EUR
Package Size: 5 x 5 mg
Fluorogenic proteasome substrate. Good for measuring the peptidylglutamyl peptide-hydrolyzing activity of the 20S proteasome.
Source: synthetic
Technical Data: min. 95,0%, Z-LLE-AMC.
Shipping / Storing Information: shipped at RT, stored at -20°C.
Productnumber: S5333.0005
changedate: 6.3.2002
Proteasome Substrate III (fluorogenic), min. 95% 5 mg - S5334.00
170,35 EUR
Package Size: 5 mg
Fluorogenic proteasome substrate. Also acts as a substrate for many other proteinases. Good for measuring the chymotrypsin-like peptidase activity of the 20S proteasome.
Source: synthetic
Technical Data: min. 95,0%, Z-LLVY-AMC.
Shipping / Storing Information: shipped at RT, stored at -20°C. …
Proteasome Substrate III (fluorogenic), min. 95% 5 x 5 mg - S533
733,44 EUR
Package Size: 5 x 5 mg
Fluorogenic proteasome substrate. Also acts as a substrate for many other proteinases. Good for measuring the chymotrypsin-like peptidase activity of the 20S proteasome.
Source: synthetic
Technical Data: min. 95,0%, Z-LLVY-AMC.
Shipping / Storing Information: shipped at RT, stored at -20°C. …
recom. Mammalian (HIS)6 Ubiquitin 1 mg - S5260.0001
197,16 EUR
Package Size: 1 mg
(His)6-Ub allows for metal chelate affinity purification of ubiquitinated molecules and for immunodetection of conjµgates using (His)6 specific antibodies.
Source: recombinant
Technical Data: min. 95%; MW = 8.5 kDa; soluble in DMSO or aqueous buffers up to 100 mg/mL.
Shipping / Storing Information: …
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