Catalogue > Services > Cloning


Cloning

Services

cDNA cloning - Cloning of PCR product - in vitro Mutagenesis - Plasmid preparation (up to 10 mg) - Plasmid preparation (up to 10 µg) - Plasmid preparation (up to 100 µg) - Plasmid preparation (up to 500 µg) - Subcloning (blunt end, < 2000 bp) - Subcloning (difficult enzymes or fragments > 2000 bp) - Subcloning (sticky end, < 2000 bp) - Synthetic genes - Transformation of plasmid-DNA - Verification sequencing (< 300 bp) - Verification sequencing (> 300 bp)


cDNA cloning set-up - P2008.0000
3.412,69 EUR Package Size: set-up Set-up price of 2499.00 Euro plus additional 0.76 Euro per bp exceeding 1000 bp.



Cloning of PCR product clone - P2002.0002
1.707,03 EUR Package Size: clone Primerdesign and synthesis. PCR with Proof-Reading Polymerase and cloning into vector. Verification of sequence plus documentation. Set-up price: 1250.00 Euro (for fragments smaller than 1000 bp) plus additional 0.51 Euro per Base exceeding 1000 bp.



in vitro Mutagenesis set-up - P2012.0000
1.340,70 EUR Package Size: set-up Service includes: Site directed Mutagenesis (Plasmid <5000 bp). Introduction of mutation. Design and synthesis of primer, mutagenesis and sequencing of mutated region (ca. 600 bp). If more than 1 mutation has to be placed, the implementation of the mutations will be subsequent. You will get all



Plasmid preparation (up to 10 mg) per plasmid - P2009.1010
1.215,40 EUR Package Size: per plasmid The mentioned amounts can only be realised together with high copy plasmids. The amounts have to be seen as maximum amounts, that can not be guaranteed.



Plasmid preparation (up to 10 µg) per plasmid - P2009.0010
17,21 EUR Package Size: per plasmid The mentioned amounts can only be realised together with high copy plasmids. The amounts have to be seen as maximum amounts, that can not be guaranteed.



Plasmid preparation (up to 100 µg) per plasmid - P2009.0100
103,45 EUR Package Size: per plasmid The mentioned amounts can only be realised together with high copy plasmids. The amounts have to be seen as maximum amounts, that can not be guaranteed.



Plasmid preparation (up to 500 µg) per plasmid - P2009.0500
376,57 EUR Package Size: per plasmid The mentioned amounts can only be realised together with high copy plasmids. The amounts have to be seen as maximum amounts, that can not be guaranteed.



Subcloning (blunt end, < 2000 bp) clone - P2005.0002
786,26 EUR Package Size: clone Subcloning of blunt-end DNA from one vector into an other using common restriction enzymes. Confirmation by restriction analysis.



Subcloning (difficult enzymes or fragments > 2000 bp) clone - P2
0,00 EUR Package Size: clone Subcloning of DNA from one vector into an other using difficult restriction enzymes or fragment sizes >2000 bp. Confirmation by restriction analysis.



Subcloning (sticky end, < 2000 bp) clone - P2005.0001
690,66 EUR Package Size: clone Subcloning of sticky DNA from one vector into an other using common restriction enzymes. Confirmation by restriction analysis.



Synthetic genes bp - P2007.0000
7,67 EUR Package Size: bp Synthesis of dsDNA and cloning into PCR cloning vector, verification by sequencing.



Transformation of plasmid-DNA per plasmid - P2010.0000
45,17 EUR Package Size: per plasmid Transformation of Plasmid-DNA into organism.



Verification sequencing (< 300 bp) clone - P2003.0001
70,26 EUR Package Size: clone sequencing of cloned construct



Verification sequencing (> 300 bp) per base - P2003.0002
0,26 EUR Package Size: per base sequencing of cloned construct



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