Expression
Services
Cold osmotic shock - Concentration of inclusion bodies - Construction of recombinant Baculo virus - Generation of high titer stock (1 L) - Generation of high titer stock (100 mL) - Generation of high titer stock (500 mL) - MOI Assay of protein production - Production of proteins from rec. Baculo-viruses (1 L) - Production of proteins from rec. Baculo-viruses (20 L) - Production of proteins from rec. Baculo-viruses (5 L) - Production of proteins from rec. Baculo-viruses (500ml) - Protein Expression (fermentor) - Protein Expression (flask) - Protein Expression Screening (E.coli). - Transient protein expression in insect cells
Cold osmotic shock 1 L - P2014.0000
365,40 EUR
Package Size: 1 L
Cold osmotic shock (1 L shake flask) to isolate periplasmatic proteins.
Cold osmotic shock Product Details Document (pdf)
Productnumber: P2014.0000
changedate: 1.1.2011
Concentration of inclusion bodies 1 L - P2013.0000
0,00 EUR
Package Size: 1 L
Price depending on complete order.
Concentration of inclusion bodies Product Details Document (pdf)
Productnumber: P2013.0000
changedate: 1.1.2011
Construction of recombinant Baculo virus clone - P2017.0000
4.987,77 EUR
Package Size: clone
Co-transfection of Sf9 insect cells with recombinant transfer vector, followed by isolation and plaque purification of 10 recombinant Baculo-viruses from supernatant. Cell infection with each recombinant and preparation of viral DNA. DNA-samples will be analysed to make sure they contain gene of interest. …
Generation of high titer stock (1 L) 1000 mL - P2018.1000
3.310,01 EUR
Package Size: 1000 mL
Comparison of protein expression efficiency of 5 rec. virus clones. Chosing most effective clone. Infection of 1.0x10(8) Sf9 cells and harvesting of supernatant after 72 hours. Second round of infection and harvesting of supernatant after 72 hours. Third round of infection in roller bottles or spinners …
Generation of high titer stock (100 mL) 100 mL - P2018.0100
1.169,23 EUR
Package Size: 100 mL
Comparison of protein expression efficiency of 5 rec. virus clones. Chosing most effective clone. Infection of 1.0x10(8) Sf9 cells and harvesting of supernatant after 72 hours. Second round of infection and harvesting of supernatant after 72 hours. Third round of infection in roller bottles or spinners …
Generation of high titer stock (500 mL) 500 mL - P2018.0500
2.093,95 EUR
Package Size: 500 mL
Comparison of protein expression efficiency of 5 rec. virus clones. Chosing most effective clone. Infection of 1.0x10(8) Sf9 cells and harvesting of supernatant after 72 hours. Second round of infection and harvesting of supernatant after 72 hours. Third round of infection in roller bottles or spinners …
MOI Assay of protein production set-up - P2019.0000
1.138,02 EUR
Package Size: set-up
Infection of rec. Virus and control virus at one MOI of 10 flasks containing Sf9 cells. Harvesting of samples for the rec. and the control viruses at 5 time points.
MOI Assay of protein production Product Details Document …
Production of proteins from rec. Baculo-viruses (1 L) 1 L - P202
2.438,61 EUR
Package Size: 1 L
Optimisation of infection parameter. Upscaling of Sf9 cell culture. Suspension culture in serum free media. Direct infection of cells with high titer stock solution (cell conc. 5x10(5) to 10(6)). Harvesting of cells and/or cell culture supernatant. Protein production control by Immuno-Western-Blot. …
Production of proteins from rec. Baculo-viruses (20 L) 20 L - P2
10.339,70 EUR
Package Size: 20 L
Optimisation of infection parameter. Upscaling of Sf9 cell culture. Suspension culture in serum free media. Direct infection of cells with high titer stock solution (cell conc. 5x10(5) to 10(6)). Harvesting of cells and/or cell culture supernatant. Protein production control by Immuno-Western-Blot. …
Production of proteins from rec. Baculo-viruses (5 L) 5 L - P202
4.877,22 EUR
Package Size: 5 L
Optimisation of infection parameter. Upscaling of Sf9 cell culture. Suspension culture in serum free media. Direct infection of cells with high titer stock solution (cell conc. 5x10(5) to 10(6)). Harvesting of cells and/or cell culture supernatant. Protein production control by Immuno-Western-Blot. …
Production of proteins from rec. Baculo-viruses (500ml) 500 mL -
1.138,02 EUR
Package Size: 500 mL
Optimisation of infection parameter. Upscaling of Sf9 cell culture. Suspension culture in serum free media. Direct infection of cells with high titer stock solution (cell conc. 5x10(5) to 10(6)). Harvesting of cells and/or cell culture supernatant. Protein production control by Immuno-Western-Blot. …
Protein Expression (fermentor) 5 litres - P2011.0000
MSRP 3.530,18 EUR
your price 110,00 EUR
Package Size: 5 litres
Expression in E.coli. Up to 5 liter working volume. High cell density fermentation (fed batch) possible. Harvest of cells. Analysis by SDS-PAGE.
Protein Expression (fermentor) Product Details Document (pdf) …
Protein Expression (flask) 1 L - P2011.1000
MSRP 665,78 EUR
your price 25,00 EUR
Package Size: 1 L
Expression in E.coli (1 L Erlenmeyer). Harvest of cells and storage. Analysis by SDS-PAGE.
Protein Expression (flask) Product Details Document (pdf)
Productnumber: P2011.1000
changedate: 1.1.2011
Protein Expression Screening (E.coli). per condition - P2011.000
MSRP 172,79 EUR
your price 2.000,00 EUR
Package Size: per condition
Parameter: pH, temperature, osmolality, etc.
Protein Expression Screening (E.coli). Product Details Document (pdf)
Productnumber: P2011.0003
changedate: 1.1.2011
Transient protein expression in insect cells 10 mL - P2025.0010
4.044,84 EUR
Package Size: 10 mL
1 gene expression under immediate early promotor including generation of expression plasmid.
Transient protein expression in insect cells Product Details Document (pdf)
Productnumber: P2025.0010
changedate: 1.1.2011
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