Expression
Services
Cold osmotic shock - Concentration of inclusion bodies - Construction of recombinant Baculo virus - Generation of high titer stock (1 L) - Generation of high titer stock (100 mL) - Generation of high titer stock (500 mL) - MOI Assay of protein production - Production of proteins from rec. Baculo-viruses (1 L) - Production of proteins from rec. Baculo-viruses (20 L) - Production of proteins from rec. Baculo-viruses (5 L) - Production of proteins from rec. Baculo-viruses (500ml) - Protein Expression (fermentor) - Protein Expression (flask) - Protein Expression Screening (E.coli). - Protein synthesised on customer demand - Proteins for crystallisation - Transient protein expression in insect cells
Cold osmotic shock 1 L
331,43 EUR
Package Size: 1 L
Cold osmotic shock (1 L shake flask) to isolate periplasmatic proteins.
Cold osmotic shock Product Details …
Concentration of inclusion bodies 1 L
0,00 EUR
Package Size: 1 L
Price depending on complete order.
Concentration of inclusion bodies Product Details Document (pdf)
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Construction of recombinant Baculo virus clone
4.524,05 EUR
Package Size: clone
Co-transfection of Sf9 insect cells with recombinant transfer vector, followed by isolation and plaque purification of 10 recombinant Baculo-viruses from supernatant. Cell infection with each …
Generation of high titer stock (1 L) 1000 mL
3.002,27 EUR
Package Size: 1000 mL
Comparison of protein expression efficiency of 5 rec. virus clones. Chosing most effective clone. Infection of 1.0x10(8) Sf9 cells and harvesting of supernatant after 72 hours. Second round of …
Generation of high titer stock (100 mL) 100 mL
1.060,53 EUR
Package Size: 100 mL
Comparison of protein expression efficiency of 5 rec. virus clones. Chosing most effective clone. Infection of 1.0x10(8) Sf9 cells and harvesting of supernatant after 72 hours. Second round of …
Generation of high titer stock (500 mL) 500 mL
1.899,28 EUR
Package Size: 500 mL
Comparison of protein expression efficiency of 5 rec. virus clones. Chosing most effective clone. Infection of 1.0x10(8) Sf9 cells and harvesting of supernatant after 72 hours. Second round of …
MOI Assay of protein production set-up
1.032,22 EUR
Package Size: set-up
Infection of rec. Virus and control virus at one MOI of 10 flasks containing Sf9 cells. Harvesting of samples for the rec. and the control viruses at 5 time points.
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Production of proteins from rec. Baculo-viruses (1 L) 1 L
2.211,89 EUR
Package Size: 1 L
Optimisation of infection parameter. Upscaling of Sf9 cell culture. Suspension culture in serum free media. Direct infection of cells with high titer stock solution (cell conc. 5x10(5) to 10(6)). …
Production of proteins from rec. Baculo-viruses (20 L) 20 L
9.378,42 EUR
Package Size: 20 L
Optimisation of infection parameter. Upscaling of Sf9 cell culture. Suspension culture in serum free media. Direct infection of cells with high titer stock solution (cell conc. 5x10(5) to 10(6)). …
Production of proteins from rec. Baculo-viruses (5 L) 5 L
4.423,78 EUR
Package Size: 5 L
Optimisation of infection parameter. Upscaling of Sf9 cell culture. Suspension culture in serum free media. Direct infection of cells with high titer stock solution (cell conc. 5x10(5) to 10(6)). …
Production of proteins from rec. Baculo-viruses (500ml) 500 mL
1.032,22 EUR
Package Size: 500 mL
Optimisation of infection parameter. Upscaling of Sf9 cell culture. Suspension culture in serum free media. Direct infection of cells with high titer stock solution (cell conc. 5x10(5) to 10(6)). …
Protein Expression (fermentor) 5 litres
3.201,98 EUR
Package Size: 5 litres
Expression in E.coli. Up to 5 liter working volume. High cell density fermentation (fed batch) possible. Harvest of cells. Analysis by SDS-PAGE.
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Protein Expression (flask) 1 L
603,88 EUR
Package Size: 1 L
Expression in E.coli (1 L Erlenmeyer). Harvest of cells and storage. Analysis by SDS-PAGE.
Protein Expression (flask) …
Protein Expression Screening (E.coli). per condition
156,73 EUR
Package Size: per condition
Parameter: pH, temperature, osmolality, etc.
Protein Expression Screening (E.coli). Product Details …
Transient protein expression in insect cells 10 mL
3.668,79 EUR
Package Size: 10 mL
1 gene expression under immediate early promotor including generation of expression plasmid.
Transient protein …
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