Protein Sequencing
Services
2D Gel electrophoresis - Automated Edman degradation of protein. - Chemical or enzymatic cleavage in solution - Fractionation of peptides by HPLC - Insuffient sample - MALDI-TOF peptide mapping - MALDI-TOF Set-up - N-terminal protein sequencing service - Proteinidentification by ESI-MS/MS - Proteolytic cleavage - SDS-Gel electrophoresis - Subsequent Degradation steps
2D Gel electrophoresis set-up
0,00 EUR
Package Size: set-up
We deliver 2D image, and sequence spots customer wants to know more about. Gel sizes from 14 cm x 20 cm up to 40 cm x 35 cm. pH range will be optimized.
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Automated Edman degradation of protein. set-up
412,89 EUR
Package Size: set-up
Degradation steps 1-5 include sample application, automatic Edman degradation and amino acid identification / verification. The ordered number of degradation steps will be billed, even if the amino …
Chemical or enzymatic cleavage in solution set-up
182,85 EUR
Package Size: set-up
Cleavage of Proteins in solution by chemicals or through peptic digest to gain smaller fragments that can be sequenced.
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Fractionation of peptides by HPLC set-up
306,72 EUR
Package Size: set-up
Fractionation of peptides by HPLC, including sample concentration, separation by reverse phase HPLC and collection of fractionated peptides.
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Insuffient sample set-up
306,72 EUR
Package Size: set-up
If sample is not sufficient to enable N-terminal sequencing, set-up fee will be billed.
Insuffient sample …
MALDI-TOF peptide mapping set-up
560,35 EUR
Package Size: set-up
Service includes sample pre-treatment, proteolytic digestion, MALDI-TOF analysis, peptide mapping, data base search and listing of hits. All data are provided on disk or e-mail.
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MALDI-TOF Set-up set-up
159,26 EUR
Package Size: set-up
Set-up includes protein digest from membrane and MALDI-TOF analysis, if MALDI-TOF gives no or not enoµgh data for further evaluation.
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N-terminal protein sequencing service set-up
1.167,88 EUR
Package Size: set-up
At least 50 pmol of a pure protein sample are required. An additional service of protein separation by SDS-PAGE prior to cleavage in gel and protein identification may be offered o request. (This is …
Proteinidentification by ESI-MS/MS set-up
0,00 EUR
Package Size: set-up
Service includes sample pre-treatment, proteolytic digestion, extraction of peptides, desalting and concentration, followed by subsequent ESI-MS/MS including complete sequencing of 2 of the peptides …
Proteolytic cleavage set-up
247,73 EUR
Package Size: set-up
Service includes sample pre-treatment, proteolytic digestion, extraction of peptide fragments from Gel or Membrane. Gels can be stained with Coomassie, but not by Silver stain.
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SDS-Gel electrophoresis set-up
129,76 EUR
Package Size: set-up
SDS-Gel for confirmation of purity of sample
SDS-Gel electrophoresis Product Details Document (pdf) …
Subsequent Degradation steps step
58,98 EUR
Package Size: step
Requirements: Protein concentration should be greater than 10 pmol. Electroblotted proteins on PVDF- or modified glass fiber (NOT nitrocellulose!) can be stained by coomassie or other dyes. Liquid / …
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