Catalogue > Services > Protein Sequencing


Protein Sequencing

Services

2D Gel electrophoresis (14 x 20cm gel) - 2D Gel electrophoresis (40 x 35cm gel) - Automated Edman degradation of protein. - Chemical or enzymatic cleavage in solution - Fractionation of peptides by HPLC - Insuffient sample - MALDI-TOF peptide mapping - MALDI-TOF Set-up - Proteinidentification by ESI-MS/MS - Proteolytic cleavage - SDS-Gel electrophoresis - Subsequent Degradation steps
2D Gel electrophoresis (14 x 20cm gel) set-up - P2055.0001


2D Gel electrophoresis (14 x 20cm gel) set-up - P2055.0001
250,00 EUR Package Size: set-up Genaxxon offers extremely high resolutioin two-dimensional gel elelctrophoresis (2-DE) for separation of protein containing samples. als Dienstleistung extrem hochauflösende zwei-dimensionale Gelelktrophorese (2DE) zur Trennung proteinhaltiger Proben an. According to 2DE gel sitze ((14 x 20cm oder 40 x


2D Gel electrophoresis (40 x 35cm gel) set-up - P2055.0002


2D Gel electrophoresis (40 x 35cm gel) set-up - P2055.0002
450,00 EUR Package Size: set-up Genaxxon offers extremely high resolutioin two-dimensional gel elelctrophoresis (2-DE) for separation of protein containing samples. als Dienstleistung extrem hochauflösende zwei-dimensionale Gelelktrophorese (2DE) zur Trennung proteinhaltiger Proben an. According to 2DE gel sitze ((14 x 20cm oder 40 x



Automated Edman degradation of protein. set-up - P2047.0000
433,53 EUR Package Size: set-up Degradation steps 1-5 include sample application, automatic Edman degradation and amino acid identification / verification. The ordered number of degradation steps will be billed, even if the amino acids cannot be identified, due to either too little protein being delivered, the N-terminus being blocked,



Chemical or enzymatic cleavage in solution set-up - P2049.0000
191,99 EUR Package Size: set-up Cleavage of Proteins in solution by chemicals or through peptic digest to gain smaller fragments that can be sequenced.



Fractionation of peptides by HPLC set-up - P2051.0000
322,05 EUR Package Size: set-up Fractionation of peptides by HPLC, including sample concentration, separation by reverse phase HPLC and collection of fractionated peptides.



Insuffient sample set-up - P2053.0000
322,05 EUR Package Size: set-up If sample is not sufficient to enable N-terminal sequencing, set-up fee will be billed.



MALDI-TOF peptide mapping set-up - P2140.0000
588,36 EUR Package Size: set-up Service includes sample pre-treatment, proteolytic digestion, MALDI-TOF analysis, peptide mapping, data base search and listing of hits. All data are provided on disk or e-mail.



MALDI-TOF Set-up set-up - P2139.0000
167,22 EUR Package Size: set-up Set-up includes protein digest from membrane and MALDI-TOF analysis, if MALDI-TOF gives no or not enoµgh data for further evaluation.



Proteinidentification by ESI-MS/MS set-up - P2141.0000
0,00 EUR Package Size: set-up Service includes sample pre-treatment, proteolytic digestion, extraction of peptides, desalting and concentration, followed by subsequent ESI-MS/MS including complete sequencing of 2 of the peptides generated by the tryptic digest. The results of the ESI-MS and the MS/MS sequencing will be checked by a



Proteolytic cleavage set-up - P2050.0000
260,12 EUR Package Size: set-up Service includes sample pre-treatment, proteolytic digestion, extraction of peptide fragments from Gel or Membrane. Gels can be stained with Coomassie, but not by Silver stain.



SDS-Gel electrophoresis set-up - P2054.0000
136,25 EUR Package Size: set-up SDS-Gel for confirmation of purity of sample



Subsequent Degradation steps step - P2048.0000
61,93 EUR Package Size: step Requirements: Protein concentration should be greater than 10 pmol. Electroblotted proteins on PVDF- or modified glass fiber (NOT nitrocellulose!) can be stained by coomassie or other dyes. Liquid / Lyophilized samples should be provided as concentrated as possible and in low salt buffers. See also



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