Protein Sequencing
Services
2D Gel electrophoresis - Automated Edman degradation of protein. - Chemical or enzymatic cleavage in solution - Fractionation of peptides by HPLC - Insuffient sample - MALDI-TOF peptide mapping - MALDI-TOF Set-up - N-terminal protein sequencing service - Proteinidentification by ESI-MS/MS - Proteolytic cleavage - SDS-Gel electrophoresis - Subsequent Degradation steps
2D Gel electrophoresis set-up - P2055.0000
0,00 EUR
Package Size: set-up
We deliver 2D image, and sequence spots customer wants to know more about. Gel sizes from 14 cm x 20 cm up to 40 cm x 35 cm. pH range will be optimized.
2D Gel electrophoresis Product Details Document (pdf) …
Automated Edman degradation of protein. set-up - P2047.0000
433,53 EUR
Package Size: set-up
Degradation steps 1-5 include sample application, automatic Edman degradation and amino acid identification / verification. The ordered number of degradation steps will be billed, even if the amino acids cannot be identified, due to either too little protein being delivered, the N-terminus being blocked, …
Chemical or enzymatic cleavage in solution set-up - P2049.0000
191,99 EUR
Package Size: set-up
Cleavage of Proteins in solution by chemicals or through peptic digest to gain smaller fragments that can be sequenced.
Chemical or enzymatic cleavage in solution Product Details Document (pdf)
…
Fractionation of peptides by HPLC set-up - P2051.0000
322,05 EUR
Package Size: set-up
Fractionation of peptides by HPLC, including sample concentration, separation by reverse phase HPLC and collection of fractionated peptides.
Fractionation of peptides by HPLC Product Details Document (pdf)
…
Insuffient sample set-up - P2053.0000
322,05 EUR
Package Size: set-up
If sample is not sufficient to enable N-terminal sequencing, set-up fee will be billed.
Insuffient sample Product Details Document (pdf)
…
MALDI-TOF peptide mapping set-up - P2140.0000
588,36 EUR
Package Size: set-up
Service includes sample pre-treatment, proteolytic digestion, MALDI-TOF analysis, peptide mapping, data base search and listing of hits. All data are provided on disk or e-mail.
MALDI-TOF peptide mapping Product Details …
MALDI-TOF Set-up set-up - P2139.0000
167,22 EUR
Package Size: set-up
Set-up includes protein digest from membrane and MALDI-TOF analysis, if MALDI-TOF gives no or not enoµgh data for further evaluation.
MALDI-TOF Set-up Product Details Document (pdf)
Productnumber: P2139.0000 …
N-terminal protein sequencing service set-up - P2052.0000
1.226,27 EUR
Package Size: set-up
Complete N-terminal Protein Sequencing Service, including Protein cleavage, Peptide fractionation by HPLC, N-terminal sequence analysis of 15 amino acids and database analysis. At least 50 pmol of a pure protein sample are required. An additional service of protein separation by SDS-PAGE prior to …
Proteinidentification by ESI-MS/MS set-up - P2141.0000
0,00 EUR
Package Size: set-up
Service includes sample pre-treatment, proteolytic digestion, extraction of peptides, desalting and concentration, followed by subsequent ESI-MS/MS including complete sequencing of 2 of the peptides generated by the tryptic digest. The results of the ESI-MS and the MS/MS sequencing will be checked by a …
Proteolytic cleavage set-up - P2050.0000
260,12 EUR
Package Size: set-up
Service includes sample pre-treatment, proteolytic digestion, extraction of peptide fragments from Gel or Membrane. Gels can be stained with Coomassie, but not by Silver stain.
Proteolytic cleavage Product Details Document …
SDS-Gel electrophoresis set-up - P2054.0000
136,25 EUR
Package Size: set-up
SDS-Gel for confirmation of purity of sample
SDS-Gel electrophoresis Product Details Document (pdf)
Productnumber: P2054.0000
changedate: 10.4.2011
Subsequent Degradation steps step - P2048.0000
61,93 EUR
Package Size: step
Requirements: Protein concentration should be greater than 10 pmol. Electroblotted proteins on PVDF- or modified glass fiber (NOT nitrocellulose!) can be stained by coomassie or other dyes. Liquid / Lyophilized samples should be provided as concentrated as possible and in low salt buffers. See also …
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