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Services

Amino Acid Analysis - Cell Line Service - Cloning - Expression - Lipopeptides - Peptide Synthesis - Protein Labelling - Protein Purification - Protein Sequencing
More sub catagories:


Amino Acid Analysis

Amino Acid Analysis from animal food - Amino Acid Analysis from physiological sample - Amino Acid Analysis of given Sample (oxidative hydrolysis) - Amino Acid Analysis of given Sample (total hydrolysis) - Amino Acid Analysis of given Sample (without total hydrolysis)



Cell Line Service





Cloning

cDNA cloning - Cloning of PCR product - in vitro Mutagenesis - Plasmid preparation (up to 10 mg) - Plasmid preparation (up to 10 µg) - Plasmid preparation (up to 100 µg) - Plasmid preparation (up to 500 µg) - Subcloning (blunt end, < 2000 bp) - Subcloning (difficult enzymes or fragments > 2000 bp) - Subcloning (sticky end, < 2000 bp) - Synthetic genes - Transformation of plasmid-DNA - Verification sequencing (< 300 bp) - Verification sequencing (> 300 bp)




Expression

Concentration of inclusion bodies - Construction of recombinant Baculo virus - Protein Expression (fermentor) - Protein Expression (flask) - Protein Expression Screening (E.coli).



Lipopeptides

Dhc-GDPKHPKSF - Dhc-SKKKK - FSL-1 - FSL-1 Ala-scan - FSL-1-Biotin - FSL-1-FLAG-tag - FSL-1-Fluorescein - FSL-1-Rhodamine - GDPKHPKSF - Pam-Dhc-GDPKHPKSF - Pam-Dhc-SKKKK - Pam2Cys-SKKKK - Pam2Cys-SKKKK(Aca-Aca-Biotin) - Pam2Cys-SKKKK(Aca-Aca-Fluorescein) - Pam2Cys-SKKKK(Aca-Aca-Rhodamine) - Pam2Cys-SKKKK-FLAG-tag - Pam3Cys-SKKKK - Pam3Cys-SKKKK (Aca-Aca-Biotin) - Pam3Cys-SKKKK (Aca-Aca-Fluorescein) - Pam3Cys-SKKKK (Aca-Aca-Rhodamine) - Pam3Cys-SKKKK-FLAG-tag - Pam3CysAQEKEAKSELDYDQT - Pam3CysGSHQMKSEGHANMQL - Pam3CysKQNVSSLDEKNSVSV - Pam3CysNNGGPELKSDEVAKS - Pam3CysNNSGKDGNTSANSAD - Pam3CysSQEPAAPAAEATPAG - Pam3CysSSGNKSAPSSSASSS - Pam3CysSSGSKPSGGPLPDAK - Pam3CysSSNAKIDQLSSDVQT - Pam3CysSSNKSTTGSGETTTA - Pam3CysSSSKSSDSSAPKAYG - Pam3CysSSSNNDAAGNGAAQT - Pam3CysSSTKPVSQDTSPKPA - PamCGDPKHPKSF - PamCSKKKK - PHC-SKKKK - PHC-SKKKK(Biotin-Aca-Aca) - R-FSL-1 - R-Pam2Cys-SKKKK - R-Pam3Cys-SKKKK - S-FSL-1 - S-Pam2Cys-SKKKK - S-Pam3Cys-SKKKK - SKKKK



Peptide Synthesis

C-terminal Biotin modification of peptide - C-terminal Fluorescein modification of peptide - Cyclisation of peptide - High grade peptide (10 mg, min. 70% pure) - High grade peptide (10 mg, min. 80% pure) - High grade peptide (10 mg, min. 95% pure) - High grade peptide (2 mg, min. 70% pure) - High grade peptide (2 mg, min. 80% pure) - High grade peptide (2 mg, min. 95% pure) - High grade peptide (20 mg, min. 70% pure) - High grade peptide (20 mg, min. 80% pure) - High grade peptide (20 mg, min. 95% pure) - High grade peptide (5 mg, min. 70% pure) - High grade peptide (5 mg, min. 80% pure) - High grade peptide (5 mg, min. 95% pure) - Immunograde peptide (10 mg, min. 55% pure) - Immunograde peptide (2 mg, min. 55% pure) - Immunograde peptide (20 mg, min. 55% pure) - Immunograde peptide (5 mg, min. 55% pure) - Isotope labelling of peptide - N-terminal Biotin modification of peptide - N-terminal Fluorescein modification of peptide - Peptide Antigen - Peptide synthesis for screening - Peptide synthesis for screening (TIP method) - Protein Antigen - Serin-phosphorylation at peptide - Serin-sulfurylation at peptide - Threonin-phosphorylation at peptide - Threonin-sulfurylation at peptide - Tyrosin-phosphorylation at peptide - Tyrosin-sulfurylation at peptide




Protein Labelling

N-terminal biotinylation of Protein (10 mg) - N-terminal biotinylation of Protein (5 mg)



Protein Purification

Affinity Protein Purification (1 L flask) - Affinity Protein Purification (fermentor) - Protein purification by IEF electrophoresis (12 cm) - Protein purification by IEF electrophoresis (25 cm) - Protein purification by SDS-gel electrophoresis



Protein Sequencing

2D Gel electrophoresis - Automated Edman degradation of protein. - Chemical or enzymatic cleavage in solution - Fractionation of peptides by HPLC - Insuffient sample - MALDI-TOF peptide mapping - MALDI-TOF Set-up - Proteinidentification by ESI-MS/MS - Proteolytic cleavage - SDS-Gel electrophoresis - Subsequent Degradation steps