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Cas9-NLS (CRISPR associated protein 9)

Cas9-NLS (CRISPR associated protein 9)

Gewicht

Hersteller Genaxxon bioscience



Artikel-Nr.: S5336.0010

shipped on wet ice, stored at -20°C

 

Lieferzeit - bitte wenden Sie sich an Genaxxon

195,00 € *
 
 
 
 
 

Produktinformationen "Cas9-NLS (CRISPR associated protein 9)"

The Genaxxon Cas9-NLS represents the wild-type Cas9 nuclease from S. pyogenes, fused with a C-terminal NLS. Currently, the best-established Cas9 protein in the field with most publications. Cas9-NLS (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes, among other bacteria. S. pyogenes utilizes Cas9 to remember and later probe and cleave foreign DNA, such as invading bacteriophage DNA or plasmid DNA. In the case that the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA. In addition to its original role in bacterial immunity, the Cas9 protein has been heavily employed as a genome engineering tool to induce site-directed double strand breaks in DNA. These disruptions may lead to gene inactivation or the presentation of heterologous genes via non-homologous end joining and homologous recombination respectively in many laboratory model organisms.

Recombinant Cas9-NLS comprises the entire Cas9 protein sequence and the nuclear localization sequence PKKKRKV (= NLS of Simian virus 40) at the C-terminus. The protein has an apparent molecular weight of 160kDa on SDS-PAGE.

Synonyms: CRISPR-associated endonuclease Cas9/Csn1, SpyCas9, cas9, csn1.

Other Cas9-NLS proteins available from Genaxxon: Cas9-NLS-tagRFP (nuclease, with red fluorescent protein tag); Cas9-Nickase-NLS (nickase); Cas9-Dead-NLS (none); Cas9-Dead-NLS-EGFP (none).

Transfection reagent for the co-transfection RNA/protein, specially designed for use in CRISPR/Cas9 systems: CRISPRfect E

 

Applikation:

CRISPR Cas9 Cloning
 
 

Unser Kommentar zu "Cas9-NLS (CRISPR associated protein 9)"

Specific genome targeting Labelling of target sites.
 

Technische Daten:

Purity: >97% (determined by SDS-PAGE).

Recombinant Cas9-NLS comprises the entire Cas9 protein sequence and the nuclear localization sequence PKKKRKV (= NLS of Simian virus 40) at the C-terminus. The protein has an apparent molecular weight of 160kDa on SDS-PAGE.

Applikation:

CRISPR Cas9 Cloning

Quelle

E.coli

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

Dokumente - Protokolle - Downloads

Hier finden Sie Infos und weiterführende Literatur zu Cas9-NLS (CRISPR associated protein 9)

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