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Cas9-NLS-tagRFP - Cas9 with red fluorescent protein tag

Cas9-NLS-tagRFP - Cas9 with red fluorescent protein tag

Gewicht

Hersteller Genaxxon bioscience



Artikel-Nr.: S5306.0010

shipped at RT, stored at -20°C

 

Lieferzeit - bitte wenden Sie sich an Genaxxon

215,00 € *
 
 
 
 
 

Produktinformationen "Cas9-NLS-tagRFP - Cas9 with red fluorescent protein tag"

As another innovative Cas9 nuclease from Genaxxon, the NLS is followed by a C-terminal tag red-fluorescent protein (tagRFP) sequence. The transfection efficiency of Cas9-NLS-tagRFP is as high as for the untagged Cas9-NLS nuclease, but the red fluorescence of successfully transfected cells allows their selection by FACS (fluorescence-activitated cell sorting). The genome editing rates of such selected cell populations is 3-4 times higher than in non-selected ones.

Excitation/emission maxima: 555 nm/584 nm

Genaxxon offers a wide range of different recombinant Streptococcus (S.) pyogenes Cas9 proteins, all of which are highly pure and effective. Fusion with a nuclear localization signal (NLS) ensures targeting of the Cas9 proteins to the nucleus. Successful genome editing by Cas9-NLS proteins has been confirmed in a wide variety of mouse and human cell lines, and organisms, including zebrafish, Xenopus and marine plankton.

Other Cas9-NLS proteins from Genaxxon: Cas9-NLS (nuclease, wildtype), Cas9-Dead-NLS (nuclease, wildtype); Cas9-Nickase-NLS (nickase); Cas9-Dead-NLS-EGFP (none)

Transfection reagent for the co-transfection RNA/protein, specially designed for use in CRISPR/Cas9 systems: CRISPRfect E

 

Applikation:

CRISPR Control of transfection efficiency of Cas9-NLS-tagRFP by FACS (fluorescence-activitated cell sorting).
 
 

Unser Kommentar zu "Cas9-NLS-tagRFP - Cas9 with red fluorescent protein tag"

Allows selection of transfected cells by sorting. 3-4 times higher indel frequencies compared with Cas9-NLS!
 

Technische Daten:

Solution of Cas9-NLS-tagRFP protein. Concentration: 0.5µg/µL (Higher concentrations are available on request). MW: 187 kDa. Shipped in storage buffer: 20mM HEPES pH7.25, 150mM KCl, 1mM DTT.

NLS from Simian virus 40 (PKKKRKV) near C-terminus followed by C-terminal RFP-tag (red fluorescent protein tag).

Applikation:

CRISPR Control of transfection efficiency of Cas9-NLS-tagRFP by FACS (fluorescence-activitated cell sorting).

Quelle

E.coli

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 34-16-04-90

Dokumente - Protokolle - Downloads

Hier finden Sie Infos und weiterführende Literatur zu Cas9-NLS-tagRFP - Cas9 with red fluorescent protein tag

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