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Cas9-Nickase-NLS

Cas9-Nickase-NLS

Gewicht

Hersteller Genaxxon bioscience



Artikel-Nr.: S5307.0010

shipped on wet ice, stored at -20°C

 

Lieferzeit - bitte wenden Sie sich an Genaxxon

195,00 € *
 
 
 
 
 

Produktinformationen "Cas9-Nickase-NLS"

Cas9-Nickases, mutant forms of the Cas9 protein, only cleave the strand complementary to the gRNA, generating a nick in the double-stranded (ds)DNA. Therefore, Cas9-Nickases need to be complexed with two different gRNAs, targeting adjacent sites on opposite DNA strands to create a double-strand break. This paired nicking action was shown to reduce off-target effects by 50 to 1,000 fold compared with the nuclease activity of the wild-type Cas9 protein (Ran et al., 2013, Cell 154: 1380-1389).

Genaxxon offers a wide range of different recombinant Streptococcus (S.) pyogenes Cas9 proteins, all of which are highly pure and effective. Fusion with a nuclear localization signal (NLS) ensures targeting of the Cas9 proteins to the nucleus. Successful genome editing by Eupheria Cas9-NLS proteins has been confirmed in a wide variety of mouse and human cell lines, and organisms, including zebrafish, Xenopus and marine plankton.

Other Cas9-NLS proteins from Genaxxon: Cas9-NLS (nuclease, wildtype), Cas9-NLS-tagRFP (nuclease, with red fluorescent protein tag); Cas9-Dead-NLS (none); Cas9-Dead-NLS-EGFP (none)

Transfection reagent for the co-transfection RNA/protein, specially designed for use in CRISPR/Cas9 systems: CRISPRfect E

 

Applikation:

CRISPR
 
 

Unser Kommentar zu "Cas9-Nickase-NLS"

Fewer off-targets than non-substituted Cas9 nucleases
 

Technische Daten:

Solution of Cas9-NLS nuclease. Concentration: 0.5µg/µL (Higher concentrations are available on request). MW of protein: 160 kDa. Shipped in storage buffer: 20mM HEPES pH7.25, 150mM KCl, 1mM DTT.

C-terminus NLS from Simian virus 40 (PKKKRKV).

D10A substitution.

Applikation:

CRISPR

Quelle

E.coli

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 34-16-04-90

Dokumente - Protokolle - Downloads

Hier finden Sie Infos und weiterführende Literatur zu Cas9-Nickase-NLS

  Spez. Produktbeschreibung

   Allg. Daten 1

 
 
 

Kundenbewertungen für "Cas9-Nickase-NLS"

 
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Die mit einem * markierten Felder sind Pflichtfelder.

 
 
Cas9-Dead-NLS-EGFP

ab 215,00 € *

GreenMasterMix (2X) High ROX für...

ab 20,00 € * Statt: 75,00 € *

Cas9-NLS-tagRFP - Cas9 with red...

ab 215,00 € *

Cas9-NLS (CRISPR associated...

ab 195,00 € *

Ribonuklease A (RNase A)

ab 41,49 € *

ProbeMasterMix (2X) No ROX für...

ab 20,00 € * Statt: 75,00 € *

CRISPRfect E...

120,00 € *

RPMI 1640 mit Glutamin -...

2,00 € * Statt: 10,14 € *

D-Xylose (min. 99,0% HPLC) -...

3,44 € * Statt: 68,88 € *

Glycin, p.A. - Restbestand

1,61 € * Statt: 32,25 € *

Collagenase Typ I - Angebot:...

30,00 € * Statt: 96,62 € *

8-cap strips, 300 strips -...

49,00 € * Statt: 98,58 € *

Bis-Tris, p.A. - Restbestand

139,00 € * Statt: 398,85 € *

Collagenase Typ II - Angebot:...

25,00 € * Statt: 96,62 € *

esiCRISPR Kit-wt

550,00 € *

Cas9-Dead-NLS

ab 195,00 € *