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Cas9-Dead-NLS

Cas9-Dead-NLS

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Hersteller Genaxxon bioscience



Artikel-Nr.: S5315.0010

shipped on wet ice, stored at -20°C

 

Lieferzeit - bitte wenden Sie sich an Genaxxon

195,00 € *
 
 
 
 
 

Produktinformationen "Cas9-Dead-NLS"

The Cas9-Dead-NLS protein is catalytically inactive ("dead") due to the D10A and H840A substitutions. Thus Cas9-Dead-NLS binds to DNA, but does not modify it. This property allows specific genomic targeting, e. g. to block transcription.

Genaxxon offers a wide range of different recombinant Streptococcus (S.) pyogenes Cas9 proteins, all of which are highly pure and effective. Fusion with a nuclear localization signal (NLS) ensures targeting of the Cas9 proteins to the nucleus. Successful genome editing by Cas9-NLS proteins has been confirmed in a wide variety of mouse and human cell lines, and organisms, including zebrafish, Xenopus and marine plankton.

Other Cas9-NLS proteins from Genaxxon: Cas9-NLS (nuclease, wildtype), Cas9-NLS-tagRFP (nuclease, with red fluorescent protein tag); Cas9-Nickase-NLS (nickase); Cas9-Dead-NLS-EGFP (none)

Transfection reagent for the co-transfection RNA/protein, specially designed for use in CRISPR/Cas9 systems: CRISPRfect E

 

Applikation:

CRISPR
 
 

Unser Kommentar zu "Cas9-Dead-NLS"

Specific genome targeting without DNA cleavage.
 

Technische Daten:

Solution of Cas9-Dead-NLS protein. Concentration: 0.5µg/µL (Higher concentrations are available on request). MW of protein: 160 kDa. Storage Buffer: 20 mM HEPES pH 7.25, 150 mM KCl, 1 mM DTT.

C-terminus NLS from Simian virus 40 (PKKKRKV).

D10A and H840A substitutions (catalytically inactive Cas9 protein).

Applikation:

CRISPR

Quelle

E.coli

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 34-16-04-90

Dokumente - Protokolle - Downloads

 
 
 

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