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Cas9-Dead-NLS-EGFP

Cas9-Dead-NLS-EGFP

Gewicht

Hersteller Genaxxon bioscience



Artikel-Nr.: S5316.0010

shipped on wet ice, stored at -20°C

 

Lieferzeit - bitte wenden Sie sich an Genaxxon

215,00 € *
 
 
 
 
 

Produktinformationen "Cas9-Dead-NLS-EGFP"

This Cas9 protein is catalytically inactive ("dead") due to the D10A and H840A substitutions. The NLS is followed by a C-terminal enhanced green-fluorescence protein (EGFP) sequence. The EGFP-tag offers many application possibilities, e.g. tracking of Cas9-Dead-NLS-EGFP.

Excitation/emission maxima: 488 nm/507 nm

Genaxxon offers a wide range of different recombinant Streptococcus (S.) pyogenes Cas9 proteins, all of which are highly pure and effective. Fusion with a nuclear localization signal (NLS) ensures targeting of the Cas9 proteins to the nucleus. Successful genome editing by Cas9-NLS proteins has been confirmed in a wide variety of mouse and human cell lines, and organisms, including zebrafish, Xenopus and marine plankton.

Other Cas9-NLS proteins from Genaxxon: Cas9-NLS (nuclease, wildtype), Cas9-NLS-tagRFP (nuclease, with red fluorescent protein tag); Cas9-Nickase-NLS (nickase); Cas9-Dead-NLS (none)

Transfection reagent for the co-transfection RNA/protein, specially designed for use in CRISPR/Cas9 systems: CRISPRfect E

 

Applikation:

CRISPR
 
 

Unser Kommentar zu "Cas9-Dead-NLS-EGFP"

Specific genome targeting without DNA cleavage. Labelling of target sites.
 

Technische Daten:

Solution of Cas9-Dead-NLS protein. Concentration: 0.5µg/µL (Higher concentrations are available on request). MW of protein: 188 kDa. Storage Buffer: 20 mM HEPES pH 7.25, 150 mM KCl, 1 mM DTT.

C-terminus NLS from Simian virus 40 (PKKKRKV) near C-terminus followed by EGFP-tag (enhanced green fluorescent protein tag).

D10A and H840A substitutions (catalytically inactive Cas9 protein).

Applikation:

CRISPR

Quelle

E.coli

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 34-16-04-90

Dokumente - Protokolle - Downloads

Hier finden Sie Infos und weiterführende Literatur zu Cas9-Dead-NLS-EGFP

  Spez. Produktbeschreibung

   Allg. Daten 1

 
 
 

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Proteinase K Lösung (20 mg/mL)

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DF Taq DNA Polymerase E (DNA-frei)

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ab 195,00 € *

DirectTissue PCR 2X Master Mix -...

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ab 25,00 € * Statt: 50,00 € *

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ab 20,00 € * Statt: 75,00 € *

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ab 0,01 € * Statt: 15,00 € *

CRISPRfect E...

120,00 € *

3x GenLadder 100 bp + 1.5 kbp...

ab 73,50 € * Statt: 110,25 € *

RPMI 1640 mit Glutamin -...

2,00 € * Statt: 10,14 € *

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Lyophilisierte qPCR Mastermix...

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