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Cas9-NLS-EGFP

Cas9-NLS-EGFP

Gewicht

Hersteller Genaxxon bioscience



Artikel-Nr.: S5325.0010

shipped on wet ice, stored at -20°C

 

Lieferzeit - bitte wenden Sie sich an Genaxxon

215,00 € *
 
 
 
 
 

Produktinformationen "Cas9-NLS-EGFP"

This innovative Cas9 nuclease is composed of a Cas9 sequence from Streptococcus pyogenes followed by a nuclear-localization sequence (NLS) and a C-terminal enhanced green fluorescent protein (EGFP) tag. Similar to Cas9-NLS-tagRFP the transfection efficiency of Cas9-NLS-EGFP protein is as high as for the untagged Cas9-NLS nuclease. The green fluorescence allows following successfully transfected cells by e.g. fluorescence-activated cell sorting (FACS) or fluorescence microscopy. Enriching successfully transfected cells by sorting increases genome editing rates and largely reduces efforts in screening and identification of edited cells.

Excitation/emission maxima: 489 nm/509 nm

Genaxxon offers a wide range of different recombinant Streptococcus (S.) pyogenes Cas9 proteins, all of which are highly pure and effective. Fusion with a nuclear localization signal (NLS) ensures targeting of the Cas9 proteins to the nucleus. Successful genome editing by Cas9-NLS proteins has been confirmed in a wide variety of mouse and human cell lines, and organisms, including zebrafish, Xenopus and marine plankton.

Other Cas9-NLS proteins from Genaxxon: Cas9-NLS (nuclease, wildtype), Cas9-NLS-tagRFP (nuclease, with red fluorescent protein tag); Cas9-Nickase-NLS (nickase); Cas9-Dead-NLS (none)

Transfection reagent for the co-transfection RNA/protein, specially designed for use in CRISPR/Cas9 systems: CRISPRfect E

 

Applikation:

CRISPR Cas9 Cloning
 
 

Unser Kommentar zu "Cas9-NLS-EGFP"

Specific genome targeting Labelling of target sites.
 

Technische Daten:

Solution of Cas9-NLS protein. Concentration: 0.5µg/µL (Higher concentrations are available on request). MW of protein: 188 kDa. Storage Buffer: 20mM HEPES pH7.25, 150mM KCl, 1mM DTT.

C-terminus NLS from Simian virus 40 (PKKKRKV) near C-terminus followed by EGFP-tag (enhanced green fluorescent protein tag).

Applikation:

CRISPR Cas9 Cloning

Quelle

E.coli

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 34-16-04-90

Dokumente - Protokolle - Downloads

Hier finden Sie Infos und weiterführende Literatur zu Cas9-NLS-EGFP

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Kundenbewertungen für "Cas9-NLS-EGFP"

 
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