Cas9-NLS-EGFP
Hersteller Genaxxon bioscience
Artikel-Nr.: S5325.0010
shipped on wet ice, stored at -20°C
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Produktinformationen "Cas9-NLS-EGFP"
This innovative Cas9 nuclease is composed of a Cas9 sequence from Streptococcus pyogenes followed by a nuclear-localization sequence (NLS) and a C-terminal enhanced green fluorescent protein (EGFP) tag. Similar to Cas9-NLS-tagRFP the transfection efficiency of Cas9-NLS-EGFP protein is as high as for the untagged Cas9-NLS nuclease. The green fluorescence allows following successfully transfected cells by e.g. fluorescence-activated cell sorting (FACS) or fluorescence microscopy. Enriching successfully transfected cells by sorting increases genome editing rates and largely reduces efforts in screening and identification of edited cells.
Excitation/emission maxima: 489 nm/509 nm
Genaxxon offers a wide range of different recombinant Streptococcus (S.) pyogenes Cas9 proteins, all of which are highly pure and effective. Fusion with a nuclear localization signal (NLS) ensures targeting of the Cas9 proteins to the nucleus. Successful genome editing by Cas9-NLS proteins has been confirmed in a wide variety of mouse and human cell lines, and organisms, including zebrafish, Xenopus and marine plankton.
Other Cas9-NLS proteins from Genaxxon: Cas9-NLS (nuclease, wildtype), Cas9-NLS-tagRFP (nuclease, with red fluorescent protein tag); Cas9-Nickase-NLS (nickase); Cas9-Dead-NLS (none)
Transfection reagent for the co-transfection RNA/protein, specially designed for use in CRISPR/Cas9 systems: CRISPRfect E
Applikation:
CRISPR Cas9 CloningUnser Kommentar zu "Cas9-NLS-EGFP"
Specific genome targeting Labelling of target sites.
Technische Daten:
Solution of Cas9-NLS protein. Concentration: 0.5µg/µL (Higher concentrations are available on request). MW of protein: 188 kDa. Storage Buffer: 20mM HEPES pH7.25, 150mM KCl, 1mM DTT.
C-terminus NLS from Simian virus 40 (PKKKRKV) near C-terminus followed by EGFP-tag (enhanced green fluorescent protein tag).
Applikation:
CRISPR Cas9 CloningQuelle
E.coliSicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 34-16-04-90Dokumente - Protokolle - Downloads
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