RealQ Plus 2x Master Mix Green - Without ROX

RealQ Plus 2x Mastermix für realtime PCR


Artikel-Nr.: A323402

Shipping: Shipped on wet ice, store at -20°C. For laboratory usage only!

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Introduction: Quantitative PCR is an important tool for SNP and gene expression analysis.... mehr
Produktinformationen "RealQ Plus 2x Master Mix Green - Without ROX"

Introduction:

Quantitative PCR is an important tool for SNP and gene expression analysis. Two general fluorescent chemistries exist for quantitative detection of gene transcripts: probes (e.g. TaqMan®, Scorpions™ Probes, molecular beacons) and DNA-binding fluorescent dyes (e.g. ethidium bromide, SYBR® Green, EvaGreen®, PicoGreen®). Ampliqon offers the RealQ Plus 2x Master Mix in two formulations: for probe or with DNA-binding fluorescent dye, making them ideal for most quantitative PCR applications.

The RealQ Plus 2x Master Mixes are available with high, low or without ROX™ for optimal performance on most of the commonly used real-time PCR instruments. The RealQ Plus 2x Master Mixes promote high specificity and low background by using TEMPase Hot Start DNA Polymerase, a modified Taq DNA polymerase with hot start capabilities.

The RealQ Plus 2x Master Mix Green, without ROX™, is a singletube 2x reagent including all components necessary to perform DNA-binding dye based real-time DNA amplification. Just add your primers and DNA.

Detection limit: approximately 1 copy.

Quantitation limit: approximately 24 copies (0.08ng of human gDNA, correlating to 12 diploid genomes, with 2 gene copies per diploid genome)

Compatibility: Real-time PCR instruments with no requirement for normalization to an internal reference dye.

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- All-in-one optimized master mix, including green dye - High sensitivity - High efficiency and high specificity - Wide dynamic range - High reproducibility - Hot Start capacity for room temperature setup
Specifications: The ProbeMastermix is a ready-to-use 2-time mastermix that contains a green... mehr
 

Technische Daten:

Specifications:
The ProbeMastermix is a ready-to-use 2-time mastermix that contains a green fluorescence dye, dUTP instead of dTTP. Contains no ROX™ as reference dye.

Applikation:

Low-copy targets PCR Real-time PCR Quantitative PCR Fast qPCR in MIC PCR Cycler

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 32-16-05-02
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