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Since the introduction of FAST qPCR, the world of real-time PCR/qPCR has entered a revolutionary era. By using special instruments and optimized master mixes aimed at speed and efficiency, this technology allows you to accelerate your qPCR applications without compromising on specificity and sensitivity.
Real-time PCR, also known as qPCR, is undoubtedly an impressive method for measuring the amplification of target DNA in real time. However, some people may despair at the abundance of terms, protocols and kits on the market. This begs the question: are reliable qPCR results only achievable through magic or is it ultimately more about the art of precise fine-tuning?
We can reassure you: in fact, with just a few tricks you can optimize your qPCR so that you can easily achieve reliable and accurate results. Whether you are already an expert in the field of qPCR or just taking your first steps into this fascinating world, this article will guide you through the key factors that will ensure your qPCR assays produce the best possible results. From deciding on the method, to quality control, to optimizing specificity - we have important tips and suggestions for you.
Do you already work with multiplex qPCR? And do you appreciate the error, cost and time savings of the multiplex qPCR method? But does your multiplex assay already deliver the best possible results? Specific quality and service features are crucial for you to perform multiplex qPCR accurately and, above all, conveniently. Thanks to the newly developed Taq DNA polymerase in our 5X qPCR Multiplex MasterMix, Genaxxon bioscience offers a solution that guarantees robust PCR performance for a wide range of different applications.
Non-protein-coding RNAs (ncRNAs) are expressed in viruses, archaea, prokaryotes and eukaryotes. They fulfil vital roles in the regulation of chromatin architecture, epigenetic memory, transcription, RNA splicing, editing and turnover. To date, at least 18 distinct types of ncRNA have been identified, which are classified according to their size, function, subcellular localization and target specificity.
Nowadays there are many different suppliers and systems for polymerase chain reaction (PCR) and of its derivative techniques, quantitative, or real-time, PCR (qPCR) and reverse transcription PCR (RT-PCR). Competition gives you the opportunity to simplify diagnostics and to make research more financially favorable.