Ni-NTA Agarose for His-tagged proteins

Ni-NTA Agarose for His-tagged proteins


Order number: S5377.0010

Shipping: Shipment: not cooled. Store at +2°C to +8°C. For laboratory usage only!

Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

€195.50 *

Volume:

Please select the favoured pack size.

Ni-NTA agarose was developed for affinity purification of proteins bearing a polyhistidine... more
Product information "Ni-NTA Agarose for His-tagged proteins"

Ni-NTA agarose was developed for affinity purification of proteins bearing a polyhistidine label. This affinity chromatography matrix is based on BioWorks workbeads consisting of 7.5% cross-linked agarose. The material is highly porous to allow optimal protein interaction. Cross-linked agarose is also physically very stable and suitable for low pressure purification processes with flow rates up to 6mL/min (optimal 0.5 - 2mL/min).

Our Ni-NTA agarose has a very homogeneous size with a mean particle diameter of 40μm, resulting in a high degree of reproducibility between individual purification runs.

Ni-NTA agarose consists of cross-linked agarose to which tetradendritic nitrilotriacetic acid (NTA) loaded with Ni2+ ions is covalently bound. The binding capacity varies with different proteins based on their specific properties, but it is at least 50mg target protein per mL agarose gel. The resin is ideally suited for batch, spin and column applications of any scale and offers a high degree of reproducibility between individual purification approaches with a very homogeneous size distribution around a mean particle diameter of 40µm.

The Ni-NTA agarose is supplied as a buffered suspension in 50% ethanol.

More products around "Ni-NTA Agarose for His-tagged proteins"
Our comment on "Ni-NTA Agarose for His-tagged proteins"
highly stable cross-linked agarose
Specifications cross-linked Agarose particle size: 32-60µm  binding capacity: 50mg/mL... more
 

Technical Data:

Specifications
cross-linked Agarose
particle size: 32-60µm 
binding capacity: 50mg/mL resin 
max pressure: 3 bar (43 psi)
pH-stability: 2-14 (short term)
pH-stability: 3–12 (long term)
flow rates: 0.5-2.0mL/min (normally) up to 6.0mL/min possible 
stable against: 100% MeOH, 100% EtOH, 8M Urea, 6M Guanidinium hydrochloride, 30% (v/v) Acetonitrile

application:

for purification of recombinant proteins for purification of His-tagged proteins

Source

synthetic

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 32-17-03-02
Dokumente - Protokolle - Downloads more


Dokumente - Protokolle - Downloads

Here you will find information and further literature on Ni-NTA Agarose for His-tagged proteins. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

 
 
 
Literatur .... more

Referenzen..

Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.

Quelle/Source:NCBI PubMed >

The UHRF1 Protein Stimulates the Activity and Specificity of the Maintenance DNA Methyltransferase DNMT1 by an Allosteric Mechanism

Pavel Bashtrykov, Gytis Jankevicius, Renata Z. Jurkowska, Sergey Ragozin, Albert Jeltsch

J Biol Chem. 2014 Feb 14; 289(7): 4106–4115.  Published online 2013 Dec 24. doi: 10.1074/jbc.M113.528893

PMCID: PMC3924276