Ni-NTA Agarose for His-tagged proteins
Order number: S5377.0010
Shipping: Shipment: not cooled. Store at +2°C to +8°C. For laboratory usage only! Delivery time: 3- 8 working days
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Ni-NTA agarose was developed for affinity purification of proteins bearing a polyhistidine label. This affinity chromatography matrix is based on BioWorks workbeads consisting of 7.5% cross-linked agarose. The material is highly porous to allow optimal protein interaction. Cross-linked agarose is also physically very stable and suitable for low pressure purification processes with flow rates up to 6mL/min (optimal 0.5 - 2mL/min).
Our Ni-NTA agarose has a very homogeneous size with a mean particle diameter of 40μm, resulting in a high degree of reproducibility between individual purification runs.
Ni-NTA agarose consists of cross-linked agarose to which tetradendritic nitrilotriacetic acid (NTA) loaded with Ni2+ ions is covalently bound. The binding capacity varies with different proteins based on their specific properties, but it is at least 50mg target protein per mL agarose gel. The resin is ideally suited for batch, spin and column applications of any scale and offers a high degree of reproducibility between individual purification approaches with a very homogeneous size distribution around a mean particle diameter of 40µm.
The Ni-NTA agarose is supplied as a buffered suspension in 50% ethanol.
highly stable cross-linked agarose
Technical Data:
Specifications
cross-linked Agarose
particle size: 32-60µm
binding capacity: 50mg/mL resin
max pressure: 3 bar (43 psi)
pH-stability: 2-14 (short term)
pH-stability: 3–12 (long term)
flow rates: 0.5-2.0mL/min (normally) up to 6.0mL/min possible
stable against: 100% MeOH, 100% EtOH, 8M Urea, 6M Guanidinium hydrochloride, 30% (v/v) Acetonitrile
application:
for purification of recombinant proteins for purification of His-tagged proteinsSource
syntheticSicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-17-03-02Dokumente - Protokolle - Downloads
Here you will find information and further literature on Ni-NTA Agarose for His-tagged proteins. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
Referenzen..
Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Quelle/Source:NCBI PubMed >
Pavel Bashtrykov, Gytis Jankevicius, Renata Z. Jurkowska, Sergey Ragozin, Albert Jeltsch
J Biol Chem. 2014 Feb 14; 289(7): 4106–4115. Published online 2013 Dec 24. doi: 10.1074/jbc.M113.528893
PMCID: PMC3924276