EDTA buffer (p8.0 - 5 bags)

Content of 1 pouch dissolved in deionized water and made up to 500mL/1000mL yields: 0.5M EDTA, pH8.0 at 25°C. EDTA (ethylene-diamine-tetraacetic acid) is a chelating agent widely used in molecular biology to sequester divalent and trivalent metal ions such as calcium and magnesium. This ability prevents DNA and RNA degradation as metal-dependent enzymes acting as nucleases become deactivated. A fully deprotonated EDTA molecule will bind directly to the metal ion making the buffer suitable for adding to stored blood as an anti-coagulant to bind Ca2+ ions. Furthermore, EDTA is useful for cell culture procedures as it prevents clumping of cells in liquid suspension and detaches adherent cells when passaging. Genaxxon’s EDTA buffer is supplied in two sizes with pouches giving 500mL or 1000mL of 0.50M EDTA buffer with pH8.0 at 25°C when the contents of one pouch is dissolved in deionized water.