Alpha MEM Eagle IPL-41 Insect Collagenase RPMI 1640 w o Trypsin powder Dulbecco s PBS Trypsin from G418 - 50mg mL DMEM with 0.5 Trypsin in Doxycycline Collagenase Schneider´s Hygromycin B RPMI 1640 w o Amphotericin B Zymolyase R Caesiumchlorid 0.05 Trypsin in DMEM w o Lympho-Paque - Serum DMEM w o RPMI 1640 with Sterile stable Serum Colcemid - 10mL Ficoll R 400 - PBS 5x with 1 IPL-41 Insect 200 mM Dulbecco s PBS Collagenase Phleomycin Gentamycin Collagenase RPMI 1640 w o Zymolyase R Penicillin-Stre TC-100 Insect Antibiotic Kanamycin Caesium Amphotericin B DMEM F12 1 1