GenLadder XLarge up to 25 kbp - ready-to-use

GenLadder XLarge up to 25 kbp - ready-to-use


Order number: GM3084.0050

Shipping: Shipment on wet ice. Store at -20°C. For laboratory usage only!

Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

€45.50 *

Weight:

Please select the favoured pack size.

With the synthetic GenLadder XLarge, even larger plasmid insertions and vector plasmids can be... more
Product information "GenLadder XLarge up to 25 kbp - ready-to-use"

With the synthetic GenLadder XLarge, even larger plasmid insertions and vector plasmids can be determined clearly and precisely in size by gel electrophoresis in the range of 250 - 25000 base pairs after restriction digests. The Genaxxon GenLadder XLarge shows 14 bands. The approximate mass of the DNA in each band is given for a 5µL load (0.52µg/lane). Additionally the 1000 bp and 3000 bp are intensified for better and faster orientation in 1.0 - 1.5% agarose gels.

Please avoid repeated freez-thaw cycles. We recommend to portion the product into smaller aliquots.

Source: 
PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH8.0) and 10mM EDTA.

GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye which contains bromophenol blue and xylene cyanol FF): M3094 (GenLadder 100 bp + 1.5 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye.

The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

More products around "GenLadder XLarge up to 25 kbp - ready-to-use"
Our comment on "GenLadder XLarge up to 25 kbp - ready-to-use"
- GenLadders for DNA sizing cover a broad range of DNA molecular weights - Sharp bands, brigth reference bands - Ready-to-use ladders are stable for 6 months at room temperature
Specifications: Number of bands: 14 Fragment sizes: 250 bp, 500 bp, 750 bp, 2x 1000 bp , 1500... more
 

Technical Data:

Specifications:
Number of bands: 14
Fragment sizes: 250 bp, 500 bp, 750 bp, 2x 1000 bp, 1500 bp, 2000 bp, 2500 bp, 2x 3000 bp, 4000 bp, 5000 bp, 6000 bp, 8000 bp, 10000 bp, 25000 bp.
DNA is dissolved at a concentration of 0.1µg/µL (52µg per 500µL).
Storage buffer: 10 mM Tris-HCl (pH8.0) and 10mM EDTA with bromophenol blue and xylene cyanol FF as tracking dyes.

We recommend to use 5µL per lane.

application:

Molecular weight standard for DNA electrophoresis

Source

synthetic

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 32-16-05-02
Dokumente - Protokolle - Downloads more


Dokumente - Protokolle - Downloads

Here you will find information and further literature on GenLadder XLarge up to 25 kbp - ready-to-use. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.