Pwo proofreading DNA polymerase
Order number: M3002.0100
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Product of the month in JUNE!
and Pfu proofreading Polymerase >
Übersicht Genaxxon
proofreading Polymerasen
und Anwendungen >
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| Quantity | Unit price |
|---|---|
| To 2 | €35.00 * |
| From 3 | €28.00 * |
| From 5 | €24.50 * |
| From 10 | €21.00 * |
*Prices plus VAT plus shipping costs
The High-Fidelity Pwo proofreading DNA polymerase from Genaxxon bioscience is a thermostable, highly processive enzyme possessing 5'-3' DNA polymerase with additional 3'-5' proofreading exonuclease activity, which enables the correction of nucleotide incorporation errors. It has no 5'→3' exonuclease activity. The High-Fidelity Pwo DNA polymerase is a recombinant form of the hyperthermophilic archaebacteria Pyrococcus woesei.
Pwo proofreading DNA polymerase shows an increased thermostability and a 10-times higher accuracy compared to Taq DNA polymerase >. A mixture of Taq DNA Polymerase and Pwo DNA polymerase provides more robust synthesis of longer amplification products (Barnes, 1994. Proc. Natl. Acad. Sci. USA 91:2216-2220).
Test sample available at a special price! The test sample price will be refunded on the first official order of the product.
Features:
- 10-times higher accuracy compared to Taq DNA polymerase
- High-Fidelity polymerase
- Proofreading function (3' - 5' exonuclease activity)
- High thermo stability
- Generates blunt-end PCR products
- Generates PCR products for cloning and expression
More High-Fidelity Proofreading Polymerases from Genaxxon bioscience:
- M3002 Pwo Proofreading Polymerase >
- M3003 ReproFast Proofreading Polymerase >
- M3004 Pfu Proofreading Polymerase >
- M3012 ReproHot (KOD) Proofreading Polymerase >
- M3030 ExactRun (Phusion like) Proofreading Polymerase >
With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.
Technical Data:
Activity: 2.5 units/µL
Proofreading polymerase (3' - 5' exonuklease activity)
application:
Proof-reading DNA polymerase for high fidelity PCR.Unit Definition:
One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C), 50 mMSource
recombinantSicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02Dokumente - Protokolle - Downloads
Here you will find information and further literature on Pwo proofreading DNA polymerase. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
Dokumente - Protokolle - Downloads
Cycle times, especially extension times, should be extended, compared to Taq DNA polymerase.
Note:
Recommended elongation time is 1 minute per 250bp of target.
Referenzen..
Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Quelle/Source: NCBI PubMed >
Systematic evaluation of error rates and causes in short samples in next-generation sequencing
Franziska Pfeiffer, Carsten Gröber, Michael Blank, Kristian Händler, Marc Beyer, Joachim L. Schultze, Günter Mayer
Sci Rep. 2018; 8: 10950. Published online 2018 Jul 19. doi: 10.1038/s41598-018-29325-6
PMCID: PMC6053417
Enjie Zhang, Victoria E. Cotton, Alberto Hidalgo-Bravo, Yan Huang, Adam J. Bell, Ruth F. Jarrett, Gavin S. Wilkie, Andrew J. Davison, Ellie P. Nacheva, Reiner Siebert, Aneela Majid, Inga Kelpanides, Sandrine Jayne, Martin J. Dyer, Nicola J. Royle
Sci Rep. 2016; 6: 22730. Published online 2016 Mar 7. doi: 10.1038/srep22730
PMCID: PMC4779988
Yan Huang, Alberto Hidalgo-Bravo, Enjie Zhang, Victoria E. Cotton, Aaron Mendez-Bermudez, Gunjan Wig, Zahara Medina-Calzada, Rita Neumann, Alec J. Jeffreys, Bruce Winney, James F. Wilson, Duncan A. Clark, Martin J. Dyer, Nicola J. Royle
Nucleic Acids Res. 2014 Jan 1; 42(1): 315–327. Published online 2013 Sep 19. doi: 10.1093/nar/gkt840
PMCID: PMC3874159
Silke Litzinger, Stefanie Fischer, Patrick Polzer, Kay Diederichs, Wolfram Welte, Christoph Mayer
J Biol Chem. 2010 Nov 12; 285(46): 35675–35684. Published online 2010 Sep 7. doi: 10.1074/jbc.M110.131037
PMCID: PMC2975192
Silke Litzinger, Amanda Duckworth, Katja Nitzsche, Christian Risinger, Valentin Wittmann, Christoph Mayer
J Bacteriol. 2010 Jun; 192(12): 3132–3143. Published online 2010 Apr 16. doi: 10.1128/JB.01256-09
PMCID: PMC2901692
