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ReproHot (KOD) proofreading Polymerase

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Manufacturer: Genaxxon bioscience



Order number: M3012.0250

shipped on wet ice, store at -20°C

 

Ready to ship today,
Delivery time appr. 1-3 workdays

€109.50 *
 
 
 
 
 

Product information "ReproHot (KOD) proofreading Polymerase"

ReproHot (KOD polymerase) is a high fidelity DNA polymerase for accurate PCR amplification of GC-rich templates used for cloning and cDNA amplification. This enzyme is a mixture of Taq DNA polymerase plus a proofreading polymerase and a monoclonal antibody, which blocks polymerase activity prior to heating. The complex formed between the polymerase and the antibody prevents primer-dimer and other artifacts resulting from unspecific primer binding. The antibody is inactivated while heating up above 75°C, requiring no prolonged first heating step prior to cycling. The combined hotstart and proofreading feature leads to highly specific and correct amplicons. ReproHot can be used as a cheap alternative to KOD-Polymerase from. e.g. Merck Millipore or other sources.

Other proofreading enzymes (Phusion, Pfu, Pwo, etc.).

With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.

 

application:

HotStart DNA polymerase for accurate PCR
 
 

Technical Data:

Anitbody based Hotstart DNA polymerase with proof-reading activity.

5 units/µL

application:

HotStart DNA polymerase for accurate PCR

Unit Definition:

One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.

Source

recombinant

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 34-16-04-90

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