Multiplex qPCR MasterMix (2x) - lyophilized
Order number: M3005.0100
Shipping: Shipment: not cooled. Store at +15°C to +30°C. For laboratory usage only! Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
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Lyophilized Multiplex qPCR MasterMix (2x) for fast and reliable quantification of up to 5 cDNA or gDNA targets in a single tube by multiplex, real-time PCR. This specially optimized and lyophilized master mix promotes fast multiplex real-time PCR. The combination of the specially designed hot start DNA polymerase together with a unique PCR buffer system as a lyophilized ready-to-use master mix ensures highly sensitive qPCR together with a very convenient storage at room temperature.
Our lyophilized Multiplex master mix for fast and easy multiplexing minimizes the need for optimization and makes the development of multiplex PCR assays fast and easy.
- Amplification of multiple targets in a single tube (tested for up to 5 targets)
- All-in-one master mix for convenient multiplexing
- High specificity, sensitivity and product yield
- Easy reaction setup at room temperature
- stable for 24 months at room temperature
The Multiplex qPCR master mix is a premixed, ready-to-use solution containing our highly specific aptamer modified Taq DNA Polymerase, highly pure dNTPs, MgCl2, additives and a reaction buffer at optimal concentrations which enable reliable multiplex PCR, e.g. microsatellite, clonality or gene rearrangement analysis. Just add your primers and template DNA.
The lyophilized Multiplex qPCR MasterMix is shipped in aliquots corresponding to 250µL of 2-fold MasterMix.
Our Standard Agarose LE > or the high resolution Agarose Tiny > sind are ideally suited for the subsequent electrophoresis analysis.
realtime master mixes for your realtime PCR experiments can be found here >.
Examples of Multiplex applications:
F. Javier Pérez-Pérez and Nancy D. Hanson, Detection of Plasmid-Mediated AmpC β-Lactamase Genes in Clinical Isolates by Using Multiplex PCR, J. Clin. Microbiol. June 2002 vol. 40 no. 6 2153-2162. doi: 10.1128/JCM.40.6.2153-2162.2002.
The Multiplex master mix for fast and easy multiplexing minimizes the need for optimization and makes the development of multiplex PCR assays fast and easy. - Amplification of multiple targets in a single tube (tested for up to 5 targets) - All-in-one master mix for convenient multiplexing - High specificity, sensitivity and product yield - Easy reaction setup at room temperature - stable at room temperature
Technical Data:
Specifications:
2-time ready-to-use lyophilised Multiplex master mix with an optimized modified Taq DNA polymerase without green fluorescent dye and without ROX™.
Aliquot size: 250µL.
The lyophilized realtime PCR master mix is stable for at least 24 months at room temperature.
application:
Multiplex PCR for Microsatellite analysis clonality analysis gene rearrangement analysisUnit Definition:
One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.Source
syntheticSicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02Dokumente - Protokolle - Downloads
Here you will find information and further literature on Multiplex qPCR MasterMix (2x) - lyophilized. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.