Products for RT-PCR

Genaxxon offers products for RNA PCR (RT-PCR = Reverse transcription polymerase chain reaction) which is still regarded as the most sensitive method for the detection and quantification of mRNA.

Compared to other conventional techniques for the quantification of mRNA - Northern Blot analysis and RNase protection assays - in RT-PCR much lower amounts of sample are needed.

The standard enyzme for the synthesis of cDNA is Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase >. M-MuLV Reverse Transcriptase shows no 3´ → 5´ exonuclease activity.
For the synthesis of cDNA Genaxxon offers also the cDNA One-Step RT-PCR Kit >.

Troubleshooting & complaints feeedback form >
Frequently asked questions (FAQs) >

Genaxxon offers products for RNA PCR (RT-PCR = Reverse transcription polymerase chain reaction) which is still regarded as the most sensitive method for the detection and quantification of mRNA.... read more »
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Products for RT-PCR

Genaxxon offers products for RNA PCR (RT-PCR = Reverse transcription polymerase chain reaction) which is still regarded as the most sensitive method for the detection and quantification of mRNA.

Compared to other conventional techniques for the quantification of mRNA - Northern Blot analysis and RNase protection assays - in RT-PCR much lower amounts of sample are needed.

The standard enyzme for the synthesis of cDNA is Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase >. M-MuLV Reverse Transcriptase shows no 3´ → 5´ exonuclease activity.
For the synthesis of cDNA Genaxxon offers also the cDNA One-Step RT-PCR Kit >.

Troubleshooting & complaints feeedback form >
Frequently asked questions (FAQs) >

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One-Step RT-PCR MasterMix (2X) One-Step RT-PCR master mix (2X) - improved
Improved version of our kit M3068. Now coming as a 2-time ready-to-use RT-PCR master mix. Our RT-PCR One-Step Kit is designed for performing highly sensitive and specific RT-PCR. The kit is based on a genetically engineered reverse...
From €372.86 *
cDNA synthesis kit - 2-step RT-PCR kit Scriptase RT - cDNA Synthesis kit
The Genaxxon bioscience Scriptase RT – cDNA Synthesis Kit contains all reagents required for first strand cDNA synthesis in one box combining simple handling with high flexibility. The premium quality Reverse Transcriptase, ultrapure...
From €326.33 *
TIPP!
Genaxx1Step RT-qPCR Covid-19 Kit Genaxx1Step RT-qPCR COVID-19 CDC Probe Assay Kit
The Genaxx1Step RT-qPCR SARS-CoV-2 CDC Probe Assay Kit is a real-time RT-PCR-based detection system for the targets N1 and N2 of the nucleocapsid from the SARS-CoV-2-Corona virus (2019-nCoV), as well as for the control target RNASeP. The...
From €246.62 *
TIPP!
GENAXXON HotScriptase mastermix with heat stable reverse Transcriptase HotScriptase Probe RT-qPCR Mastermix without ROX
HotScriptase Probe Mastermix for RT-PCR without an isothermal intermediate step, thereby saving time and money: HotScriptase Probe Mastermix was successfully used by several customers for the detection of RNA viruses like Zika or...
From €169.38 *
One-Step RT-qPCR 2X GreenMastermix One-Step RT-qPCR 2X GreenMastermix
Our One-Step RT-qPCR 2X master mix was developed for quantitative real-time analyzes of RNA templates using EvaGreen®. The ready-to-use mixture is based on a genetically modified reverse transcriptase with improved thermal stability,...
From €479.54 *
one-step RT-PCR scheme - realtime RT-PCR kit One-Step RT-qPCR 2X ProbeMastermix
The One-Step RT-qPCR 2X ProbeMastermix without ROX® was developed for quantitative real-time analyzes of RNA templates with double-labelled fluorescence probes. The ready-to-use mixture is based on an optimized genetically modified...
From €479.54 *
random Hexamer N6 Primer 5OD (160µg) Random Hexamer Primer N6
Random Hexamers are short oligodeoxyribonucleotides of random sequence [d(N)6]. Primers are quality controlled (MS), purified (reversed phase HPLC), lyophilised and aliquoted and came with a guaranteed amount of at least 5OD (about...
From €21.66 * €28.88 *
Oligo dT20 Primer 5OD (130µg) Oligo dT20 primer
Oligo (dT)20 are single stranded oligodeoxyribonucleotides containing only deoxythymine (dT) to be able to prime with the poly(A) tail of mRNA molecules. Primers are quality controlled (MS), purified (reversed phase HPLC), aliquoted and...
From €10.15 * €14.50 *
recombinant RNase Inhibitor - RNasin recombinant RNase Inhibitor - RNasin
RNase inhibitor (ribonuclease inhibitor / RNasin) is a broad-spectrum inhibitor of RNases that is reversibly inactivated by binding to the inhibitor at a 1: 1 ratio with an association constant greater than 10E14. RNase A / B / C and...
From €99.50 *
M-MuLV Reverse Transcriptase RNase H negative M-MuLV Reverse Transcriptase RNase H negative
The Genaxxon M-MuLV Reverse Transcriptase, encoded by Moloney Murine Leukemia Virus (M-MuLV RT) and expressed in E.coli is an RNA-dependent DNA polymerase lacking the RNase H activity. The enzyme synthesizes the cDNA first strand from a...
From €122.69 *
HotScriptase - RT Mastermix PCR protocol HotScriptase RT Mastermix with GreenDye without...
HotScriptase RT Master Mix for One-Step RT-PCR without isothermal reverse transcription step. Using HotScriptase RT is very time and cost efficienct as HotScriptase RT is a reverse transcriptase and a DNA Polymerase combined in one...
From €170.98 *