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pMBL TA-Cloning Kit
01.12.2005
T/A Cloning Kit for cloning of DNA-fragments with A-overhangs.
Product-page - Produkt-Seite (deutsch)
The pMBL-T/A Cloning Kit is a convenient system for the cloning of PCR products. The single 3´-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid by preventing recircularization of the vector and providing a compatible overhang for ligation of PCR products generated by certain thermostable polymerases.The high copy number pMBL vector contains a T3 RNA polymerase promoter in the 3´-region of the MCS for in vitro RNA transcription. Insertional inactivation of the alpha-peptide allows recombinant clones to be directly identified by color screening on indicator plates. The pMBL TA Cloning Kit can be ordered with the product number M3164.
