Pisum sativum lectin (PSA)

Pisum sativum lectin (PSA) - structure


Artikel-Nr.: D1517.0010

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Pisum sativum lectin or agglutinin (PSA) is isolated from peas and purified by affinity... mehr
Produktinformationen "Pisum sativum lectin (PSA)"

Pisum sativum lectin or agglutinin (PSA) is isolated from peas and purified by affinity chromatography. The lectin has four subunits and a molecular weight of 49 kDa. PSA agglutinates human erythrocytes but is not blood group specific. It displays specificity toward α-D-mannose and α-D-glucose and has mitogenic activity similar to Concanavalin A.

Pisum sativum lectin is supplied as a white to cream coloured lyophilized powder. The purity of PSA is determined by SDS- electrophoresis, which generates two bands at 17 kDa and 5,7 kDa corresponding to the α- and ß-chains that are formed in solution, below pH5.8.

How to use
The lectin may be reconstituted with 2mL of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol.

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10-100 μg/mL lectin is required to agglutinate neuraminidase treated erythrocytes. Agglutinates human erythrocytes; not blood group specific.
Specifications: Appearance: White to cream-coloured lyophilized powder. Protein content:... mehr
 

Technische Daten:

Specifications:
Appearance: White to cream-coloured lyophilized powder.
Protein content: >85%
Identity: two major bands in SDS-PAGE
MW : 49 kDa (4 subunits of 12.25 kDa each)
Sugar specifity: α-D-Man and α-D-Glu
PSA agglutinates human erythrocytes but is not blood group specific.
It displays specificity toward α-D-mannose and α-D-glucose and has mitogenic activity similar to Concanavalin A.

Applikation:

Agglutination studies Model system of how proteins recognize carbohydrates

Quelle

pea

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 34-16-04-90
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Hier finden Sie Informationen und weiterführende Literatur zu Pisum sativum lectin (PSA). Für weitere Dokumente (Zertifikate mit weiteren Lotnummern, Sicherheitsdatenblätter in anderer Sprache, weitere Produktinformationen) wenden Sie sich bitte an Genaxxon biosience unter: info@genaxxon.com oder Tel.: +49 731 3608 123.