Genaxxon’s Exosome Maxi Purification Kit ensures high efficiency in exosome and extracellular vesicle (EV) isolation. Designed to purify exosomes from cell culture medium, this kit leverages advanced magnetic bead technology. Achieve results comparable to or better than ultracentrifugation, without the need for specialized equipment. The kit efficiently isolates exosomes using magnetic beads that adsorb exosomes during purification and release them in the elution buffer, ensuring high purity and yield. These eluted exosomes are ideal for cell culture applications and animal injections and are suitable for immediate functional analyses such as NTA. Applications The Genaxxon Serum Exosome Purification Kit is versatile and can be used to isolate microvesicles from cell culture medium. The isolated vesicles are perfect for a range of analytical and functional applications, including: Physical Characterization: Techniques such as Nanoparticle Tracking Analysis (NTA) and Tunable Resistive Pulse Sensing (TRPS) Electron MicroscopyIsolation and Analysis: Suitable for DNA, RNA, proteins, lipids, and other constituentsFlow CytometryAntibody- or Fluorescent Dye-Based LabelingUptake by Recipient Cells For smaller sample volumes, the kit allows for volume adjustment using ddH2O, maintaining flexibility and efficiency in your experimental setups. Choose the Genaxxon Exosome Maxi Purification Kit for reliable, high-quality exosome isolation, enabling advanced research and application in various biological fields.Genaxxon also offers a Exosome Protein Purification Kit (S5328), and other Exosome purification kits for your specific needs (Exosome Mini Purification Kit (S5321) for small-volume samples or the Serum Exosome Purification Kit (S5323)).

Leere Chromatographiesäulen für Standard-Proteinaufreinigungsprozesse wie z.b. Affinitätschromatographie mit Ni-IDA, Ni-NTA, Co-IDA oder Co-NTA-Agarosen, mittels "gravity-flow" oder Zentrifugation. Die S-Säulen (0,5mL) > sind ideal für Optimierungsscreenings Ihrer Proteinaufreinigung geeignet. Mit L-Säulen (20mL) können Sie sehr kostengünstig die Agarose in Bulkmengen einkaufen und angepasst an ihre Anwendung entsprechende Proteinaufreinigungssäulen packen. Ideal für die Anwendung der Genaxxon Niedrigdruck-Bulk-Agarosen. Verfügbar in den Größen: 0,5mL (S),  und 20mL (L). Einfaches Handling. Verwendbar unter nativen wie denaturienden Bedingungen. Für die Anwendung werden Zentrifugen mit festem Rotor (45° für die 500µL-Säulchen) und mit Ausschwingrotor (für die 20mL Säulchen) benötigt.

Genaxxon’s Exosome Mini Purification Kit ensures high efficiency in exosome and extracellular vesicle (EV) isolation. Designed to purify exosomes from small volumes (0.69mL per sample) of major biofluids such as serum, blood plasma, and other exosome-rich samples, this kit leverages advanced magnetic bead technology. Achieve results comparable to or better than ultracentrifugation, without the need for specialized equipment. The kit efficiently isolates exosomes using magnetic beads that adsorb exosomes during purification and release them in the elution buffer, ensuring high purity and yield. These eluted exosomes are ideal for cell culture applications and animal injections and are suitable for immediate functional analyses such as NTA.Features and Benefits • Short purification time (2h) and good integrity of purified exosomes• Not contain other compounds and used directly in cell culture experiments and animal in vivo injection experiments• High purity and low levels of exogenous contaminant proteins• Simple and efficient, suitable for processing large quantities of samplesApplications The Genaxxon Exosome Mini Purification Kit is versatile and can be used to isolate microvesicles from serum, plasma, cell culture supernatant or other biological liquid samples. The isolated vesicles are perfect for a range of analytical and functional applications, including: Physical Characterization: Techniques such as Nanoparticle Tracking Analysis (NTA) and Tunable Resistive Pulse Sensing (TRPS) Electron MicroscopyIsolation and Analysis: Suitable for DNA, RNA, proteins, lipids, and other constituentsFlow CytometryAntibody- or Fluorescent Dye-Based LabelingUptake by Recipient Cells For smaller sample volumes, the kit allows for volume adjustment using ddH2O, maintaining flexibility and efficiency in your experimental setups. Choose the Genaxxon Exosome Mini Purification Kit for reliable, high-quality exosome isolation, enabling advanced research and application in various biological fields.Genaxxon also offers a Exosome Protein Purification Kit (S5328 >), and other Exosome purification kits for your specific needs (Serum Exosome Purification Kit (S5323 >) or the best selling product Exosome Maxi Purification Kit (S5326 >).

Genaxxon’s Serum Exosome Purification Kit ensures high efficiency in exosome and extracellular vesicle (EV) isolation. Designed to purify exosomes from serum and blood plasma, this kit leverages advanced magnetic bead technology. Achieve results comparable to or better than ultracentrifugation, without the need for specialized equipment. The kit efficiently isolates exosomes using magnetic beads that adsorb exosomes during purification and release them in the elution buffer, ensuring high purity and yield. These eluted exosomes are ideal for cell culture applications and animal injections and are suitable for immediate functional analyses such as NTA. Applications The Genaxxon Serum Exosome Purification Kit is versatile and can be used to isolate microvesicles from serum, plasma, or other biological liquid samples. The isolated vesicles are perfect for a range of analytical and functional applications, including: Physical Characterization: Techniques such as Nanoparticle Tracking Analysis (NTA) and Tunable Resistive Pulse Sensing (TRPS) Electron MicroscopyIsolation and Analysis: Suitable for DNA, RNA, proteins, lipids, and other constituentsFlow CytometryAntibody- or Fluorescent Dye-Based LabelingUptake by Recipient Cells The exosome purification system provided in this kit is capable of purifying 20 ml of serum, plasma, or other highly viscous samples, meeting various experimental requirements such as exosomal protein extraction, RNA extraction, and miRNA extraction. This kit is not suitable for extracting exosomes from highly concentrated cell culture medium. Choose the Genaxxon Serum Exosome Purification Kit for reliable, high-quality exosome isolation, enabling advanced research and application in various biological fields.Genaxxon also offers a Exosome Protein Purification Kit (S5328), and other Exosome purification kits for your specific needs (Exosome Mini Purification Kit (S5321) for small-volume samples or the best selling product Exosome Maxi Purification Kit (S5326).

Genaxxon's Exosome Protein Extraction Kit is especially engineered to extract proteins from various exosomes with high efficiency. The kit is the perfect solution for researchers aiming for high-quality Western Blot results.Key Features: High Protein Yield: Achieve a high concentration of exosome proteins, ensuring optimal results for your Western Blot experiments. Effective Lysis: The kit contains a special protein denaturant that effectively lyses exosomes, maximizing protein extraction. Easy to Use: Simply mix the kit with your exosome solution to lyse the exosomes. The lysate can then be directly loaded into SDS-PAGE for straightforward analysis. Accurate Quantification: Measure the concentration of exosome proteins in the lysates using either the Bradford or BCA protein concentration assay methods. Western Blot Ready: The denatured proteins are perfectly suited for Western Blot applications, ensuring reliable and high-quality results. Note: These proteins are not suitable for ELISA or enzyme activity measurements. Applications: Western Blot Analysis: Ideal for researchers focused on obtaining high-quality Western Blot data. Protein Quantification: Compatible with Bradford and BCA assay methods for accurate protein concentration measurement. Choose the Genaxxon Exosome Protein Extraction Kit for a reliable, efficient, and high-yield extraction of exosome proteins, tailored specifically for Western Blot success. 

Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments We use magnetic beads with a ferrimagnetic core and an agarose coating coupled to a ligand of choice, our beads meet our own high quality standards. They enable fast and easy purification steps, which can be automated. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. Genaxxon MagBeads are ferrimagnetic agarose beads coupled to a chelating ligand (IDA or NTA) coordinating nickel or cobalt ions. All offered ligand-metal systems efficiently bind histidine-tagged proteins. With a binding capacity of up to 40mg protein per mL (Ni-IDA) and 70mg per mL (Ni-NTA) of settled MagBeads, Genaxxon Ni-IDA MagBeads are comparable to equivalent beads from alternative providers.

Co-NTA-Agarose für His-tagged Proteine besteht aus quervernetzter Agarose, an die mit Co2+-Ionen beladene tetradendritische Nitrilotriessigsäure (NTA) kovalent gebunden ist. Bei der Aufarbeitung von rekombinanten Fusionsproteinen aus Eukaryonten oder im Falle von speziellen Proteinkomplexen sollte aufgrund der höheren zu erzielenden Reinheit der Proteine grundsätzlich Co-NTA Agarose verwendet werden. Co2+ bindet nur Domänen mit benachbarten Histidinresten und ist somit äußerst selektiv für die 6-fach Histidin-getaggten Fusionsproteine. Ni2+-Ionen hingegen sind weniger spezifisch und können Histidin-Reste auch außerhalb des Polyhistidin-Tags binden, was zu einer stärkeren Verunreinigung mit Fremdproteinen führen kann. Der einzigartige Herstellungsprozess der Genaxxon NTA Agarose ergibt eine Co-NTA-Agarose mit einer sehr hohen Bindekapazität. Die Genaxxon Co-NTA Agarose zeigt sich in Gegenwart von DTT und EDTA sehr robust. In einem Stabilitätstest wurde die Genaxxon Co-NTA Agarose mit steigenden Konzentrationen an DTT und EDTA für eine Stunde inkubiert. Anschließend wurde die Agarose für die Batch-Reinigung von in E.coli exprimierten GFP-His verwendet. Die Bindekapazität verringerte sich in Gegenwart von DTT und EDTA, jedoch zeigen die gemessenen Werte einen weniger steilen Abfall, als bei der Konkurrenz. Die Genaxxon NTA Agarose wird als 50%ige, gepufferte Suspension in 20% Ethanol geliefert.

Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments We use magnetic beads with a ferrimagnetic core and an agarose coating coupled to a ligand of choice, our beads meet our own high quality standards. They enable fast and easy purification steps, which can be automated. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. Genaxxon MagBeads are ferrimagnetic agarose beads coupled to a chelating ligand (IDA or NTA) coordinating nickel or cobalt ions. All offered ligand-metal systems efficiently bind histidine-tagged proteins. With a binding capacity of up to 40mg protein per mL (Ni-IDA) and 70mg per mL (Ni-NTA) of settled MagBeads, Genaxxon Ni-IDA MagBeads are comparable to equivalent beads from alternative providers.

Ni-NTA-Agarose wurde für die Affinitätsreinigung von Proteinen entwickelt, die eine Polyhistidin-Markierung tragen. Diese Affinitätschromatographie-Matrix basiert auf BioWorks-Workbeads, die aus 7,5% vernetzter Agarose bestehen. Das Material ist hochporös, um eine optimale Proteininteraktion zu ermöglichen. Vernetzte Agarose ist auch physikalisch sehr stabil und eignet sich für Reinigungsprozesse unter niedrigem Druck mit Flussraten von bis zu 6mL/min (optimal 0,5 - 2mL/min). Unsere Ni-NTA-Agarose hat eine sehr homogene Größe mit einem mittleren Partikeldurchmesser von 40μm, was zu einem hohen Grad an Reproduzierbarkeit zwischen einzelnen Reinigungsdurchläufen führt. Ni-NTA-Agarose besteht aus quervernetzter Agarose, an die mit Ni2+-Ionen beladene tetradendritische Nitrilotriessigsäure (NTA) kovalent gebunden ist. Die Bindungskapazität variiert mit den unterschiedlichen Proteinen aufgrund deren spezifischen Eigenschaften, wobei sie aber mindestens bei 50mg Zielprotein pro mL Agarose-Gel liegt. Das Harz ist bestens geeignet für die Einsatzbereiche Batch-, Spin- und Säulenverfahren jeglichen Maßstabs und bietet mit einer sehr homogenen Größenverteilung um einen mittleren Partikeldurchmesser von 40µm einen hohen Grad an Reproduzierbarkeit zwischen einzelnen Reinigungsansätzen. Die Ni-NTA Agarose wird als gepufferte Suspension in 50% Ethanol geliefert. So entsprechen, z.B. 1x S5377.0010 20mL einer Suspension aus 10mL Resin und 10mL Wasser mit 20% EtOH.

Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments We use magnetic beads with a ferrimagnetic core and an agarose coating coupled to a ligand of choice, our beads meet our own high quality standards. They enable fast and easy purification steps, which can be automated. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. Genaxxon MagBeads are ferrimagnetic agarose beads coupled to a chelating ligand (IDA or NTA) coordinating nickel or cobalt ions. All offered ligand-metal systems efficiently bind histidine-tagged proteins. With a binding capacity of up to 40mg protein per mL (Ni-IDA) and 70mg per mL (Ni-NTA) of settled MagBeads, Genaxxon Ni-IDA MagBeads are comparable to equivalent beads from alternative providers.

Nickel-beladene Agarosen sind die am häufigsten eingesetzten Produkte zur effizienten Aufreinigung von 6-fach Histidin-getaggten rekombinanten Fusionsproteinen. Die Ni2+-Ionen weisen eine hohe Affinität zu 6-fach Histidin-getagten Fusionsproteinen auf und führen zu sehr hohen Rohausbeuten bei der Aufreinigung dieser Fusionsproteine aus den Lysaten. Wird jedoch aus Lysaten aufgereinigt, die auch Fremdproteine mit wiederkehrenden Histidin- Anteilen in den Ketten enthalten, so nimmt die Selektivität gegenüber den 6-fach Histidin-getagten Fusionsproteinen ab, da zunehmend auch nicht-getagte Histidin-enthaltende Proteine an die Ni-IDA-Agarose binden. In diesem Fall empfiehlt sich die Verwendung von Genaxxon Co-IDA-Agarose (Kat.-Nr. S5370), die eine weit höhere Selektivität gegenüber 6-fach Histidin-getagten Fusionsproteinen aufweist. Genaxxon Ni-IDA-Agarose zeichnet sich durch hohe chemische Stabilität sowie durch die einfache Möglichkeit aus, die Aufreinigung direkt aus den Rohlysaten durchzuführen. Die Genaxxon Ni-IDA-Agarose kann darüber hinaus mehrfach mit Nickelsulfat-Lösung regeneriert und wieder eingesetzt werden. Ni-IDA-Agarose - Der effiziente, aber günstige Weg zur Aufreinigung von 6x Histidin-getagten Fusionsproteinen. Highlights: - ideal geeignet für Grafity-Flow- und Batch-Verfahren - Kein teures Aufreinigungssystem notwendig - konsistente Leistungsfähigkeit - für native wie auch denaturierende Bedingungen - Extrem kostengünstig (Volumenangaben entsprechen den Agarosemengen)! Die Genaxxon Ni-IDA Agarose besteht aus quervernetzter Agarose, an die mit Ni2+-Ionen beladene Iminodiessigsäure (IDA) kovalent gebunden ist. Bindungskapazität bis zu 50mg 6xHistidin-getagtes Fusionsprotein pro mL Agarose. Die Ni-IDA Agarose wird als eine gepufferte 50% Suspension mit 20% Ethanol geliefert.

Leere Zentrifugen-Säulen für Standard-Proteinaufreinigungsprozesse, wie z.b. Affinitätschromatographie mit Ni-IDA, Ni-NTA, Co-IDA oder Co-NTA-Agarosen. Die S-Säulen (0,5mL) sind ideal für Optimierungsscreenings Ihrer Proteinaufreinigung geeignet. Mit L-Säulen (20mL) > können Sie sehr kostengünstig die Agarose in Bulkmengen einkaufen und angepasst an ihre Anwendung entsprechende Proteinaufreinigungssäulen packen. Ideal für die Anwendung der Genaxxon Niedrigdruck-Bulk-Agarosen. Verfügbar in den Größen: 0,5mL (S),  und 20mL (L). Einfaches Handling. Verwendbar unter nativen wie denaturienden Bedingungen. Für die Anwendung werden Zentrifugen mit festem Rotor (45° für die 500µL Säulchen) und mit Ausschwingrotor (für die 20mL Säulchen) benötigt.

Genaxxon bietet gebrauchsfertige Ni2+ IDA Säulchen für die His-tag Proteinaufreinigung im Gravity-Flow-Verfahren an. Damit ist ein schneller Aufreinigungsprozess mit hoher Ausbeute an Zielprotein verfügbar ohne lästiges Packen zur Verfügung stehender Leersäulen. Es werden beide Matrices, Ni2+- wie auch Co2+-IDA-Agarose, mit zwei verschiedenen Füllbettvolumina (1mL, 5mL) als 20% ethanolische Suspension angeboten. Frittenporengröße 20 µm - Agarose 6% quervernetzte Agarose (Sepharose®CL-6B) - Matrixstabilität in allen für die Proteinaufreinigung üblichen Reagenzien - Aktivierungsagenz Epichlorhydrin - Bettvolumen: Agarose 1mL (für L-Säulen), 5mL (für XL-Säulen) - Kapazität ca. 28 µmol Ni2+/mL Gel, ca. 25 µmol Co2+/mL Gel.

CentriPure P100 Säulchen sind Gelfiltrationssäulchen für die schnelle und effiziente Proteinreinigung und -entsalzung. Geeignet für Auftragsvolumina bis zu 10mL. CentriPure P100 Gelfiltrationssäulchen sind extra für die schnelle und effiziente Abtrennung kleiner Moleküle (Salzen, Farbstoffen, Ammonium, Haptenen, Biotin, etc.) von Antikörpern, Enzymen oder anderen Proteinen entwickelt worden. Die Gelmatrix von CentriPure besteht aus Zetadex-25, einem kugelförmigen Kompositmaterial. Das Material zeichnet sich durch hohe Selektivität, hohe Auflösung und chemische Stabilität aus. Die Trennung basiert auf der Größentrennung der Moleküle. Kleinere Moleküle werden wesentlich später eluiert als größere Moleküle. Puffer und pH haben auf die Trennung höchstens einen minimalen Einfluß. Der Größenausschluß bei Zetadex-25 beträgt 5 kD für Proteine. Proteine mit einem Molgewicht von größer 5 kD können in einem Auftragsvolumen von bis zu 10mL können gereinigt werden indem man die ersten 12mL bis 15mL des Eluats auffängt.

CentriPure P50 Säulchen sind Gelfiltrationssäulchen für die schnelle und effiziente Proteinreinigung und -entsalzung. Geeignet für Auftragsvolumina bis zu 5mL. CentriPure P50 Gelfiltrationssäulchen sind extra für die schnelle und effiziente Abtrennung kleiner Moleküle (Salzen, Farbstoffen, Ammonium, Haptenen, Biotin, etc.) von Antikörpern, Enzymen oder anderen Proteinen entwickelt worden. Die Gelmatrix von CentriPure besteht aus Zetadex-25, einem kugelförmigen Kompositmaterial. Das Material zeichnet sich durch hohe Selektivität, hohe Auflösung und chemische Stabilität aus. Die Trennung basiert auf der Größentrennung der Moleküle. Kleinere Moleküle werden wesentlich später eluiert als größere Moleküle. Puffer und pH haben auf die Trennung höchstens einen minimalen Einfluß. Der Größenausschluß bei Zetadex-25 beträgt 5 kD für Proteine. Proteine mit einem Molgewicht von größer 5 kD können in einem Auftragsvolumen von bis zu 5mL können gereinigt werden.

CentriPure P5 Säulchen sind vorgewässerte Gelfiltrationssäulchen für die Proteinreinigung und -entsalzung. Geeignet für Produktvolumina von 500µL. CentriPure P5 Gelfiltrationssäulchen sind extra für die schnelle und effiziente Abtrennung kleiner Moleküle (Salzen, Farbstoffen, Ammonium, Haptenen, Biotin, etc.) von Antikörpern, Enzymen oder anderen Proteinen entwickelt worden. Die Gelmatrix von CentriPure besteht aus Zetadex-25, einem kugelförmigen Kompositmaterial das von emp Biotech entwickelt wurde. Das Material zeichnet sich durch hohe Selektivität, hohe Auflösung und chemische Stabilität aus. Die Trennung basiert auf der Trennung duch Größe der Moleküle. Kleinere Moleküle werden wesentlich später eluiert als größere Moleküle. Puffer und pH haben auf die Trennung höchstens einen minimalen Einfluß. Der Größenausschluß bei Zetadex-25 beträgt 5 kDa für Proteine. Proteine mit einem Molgewicht von größer 5 kDa, aufgetragen in einem Auftragsvolumen von 450 - 500µL, können gereinigt werden.

Gewässerte Gelfiltrationssäulchen für die Proteinreinigung und -entsalzung. Geeignet für Produktvolumina von 150µL bis 300µL. CentriPure 2 Gelfiltrationssäulchen sind extra für die schnelle und effiziente Abtrennung kleiner Moleküle (Salzen, Farbstoffen, Ammonium, Haptenen, Biotin, etc.) von Antikörpern, Enzymen oder anderen Proteinen entwickelt worden. Die Gelmatrix von CentriPure besteht aus Zetadex-25, einem kugelförmigen Kompositmaterial das von emp Biotech entwickelt wurde. Das Material zeichnet sich durch hohe Selektivität, hohe Auflösung und chemische Stabilität aus. Die Trennung basiert auf der Trennung duch Größe der Moleküle. Kleinere Moleküle werden wesentlich später eluiert als größere Moleküle. Puffer und pH haben auf die Trennung höchstens einen minimalen Einfluß. Der Größenausschluß bei Zetadex-25 beträgt 5 kDa für Proteine. Proteine mit einem Molgewicht von größer 5 kDa aufgetragen in einem Auftragsvolumen von 150µL bis 300µL können gereinigt werden indem man das Elutionsvolumen von 200µL bis 350µL auffängt.

Gewässerte CentriPure Z25 Gelfiltrationssäulchen für die DNA und/oder Proteinreinigung und -entsalzung. Geeignet für Volumina von bis zu 100µL. CentriPure Mini Gelfiltrationssäulchen sind extra für die schnelle und effiziente Abtrennung kleiner Moleküle (Pufferaustausch und/oder Entfernung von Farbstoffen, Haptenen und anderen kleinen Molekülen) von Proteinen größer 5 kDa konzipiert. Die gereinigten Proteine eluieren in reines Wasser oder Puffer (ACHTUNG: Einige Proteine präzipitieren bei Elution in Lösung mit niederiger Ionenstärke (z.B. reines Wasser)). Die Gelmatrix von CentriPure Säulchen besteht aus Zetadex-25, einem kugelförmigen Kompositmaterial das von emp Biotech entwickelt wurde. Das Material zeichnet sich durch hohe Selektivität, hohe Auflösung und chemische Stabilität aus. Die Trennung basiert auf der Trennung duch Größe der Moleküle. Kleinere Moleküle werden wesentlich später eluiert als größere Moleküle. Puffer und pH haben auf die Trennung höchstens einen minimalen Einfluß. Der Größenausschluß bei Zetadex-25 beträgt 5 kDa für Proteine.

Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments. We use magnetic beads with a ferrimagnetic core and an agarose coating coupled to a ligand of choice, our beads meet our own high quality standards. They enable fast and easy purification steps, which can be automated. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. The rho1D4 purification system is based on the highly specific binding of the rho1D4 antibody to the rho1D4 epitope fused to proteins. This system has proven particularly effective with membrane proteins. Magnetic beads are useful for protein purification of dilute samples, and for pull-down experiments to determine protein-protein interactions. Elution of the protein can be done the rho1D4 peptide, which competitively binds to the rho1D4 antibody, thereby release the traget protein. The peptide can be purchased individually, or together with Genaxxon Rho1D4 MagBeads as part of a Starter Set. A 5% suspension is provided that binds up to 3mg of protein per mL settled beads. Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.

Set of Rho1D4 agarose plus the Rho1D4 peptide for elution. Proteins tagged with the epitope sequence of the Rho1D4 antibody can be purified with this affinity matrix. Rho1D4 peptide in the Elution Buffer serves to competitively bind to the affinity resin and release the tagged protein, providing gentler elution conditions than, for example, changing pH. Genaxon bioscience offers conveniently aliquoted Rho1D4 peptide that has been tested with Rho1D4 Agarose. Based on BioWorks Workbeads, this product is the first commercially available immunoaffinity resin for the Rho1D4 purification system. Properties: - demonstrably suitable for membrane protein research - binding capacity of 3-4mg protein per mL resin - purifies protein with high specificity - gentle protein elution based on competitive binding - can be regenerated for reuse. Genaxxon resins are produced under strict quality guidelines and each batch undergoes quality checks to ensure that the loaded matrix has a high protein capacity. Combined with the specificity of the antibody-epitope interaction, a purification protocol optimized for the target protein can generate elution fractions with exceptionally high yields. This set offers both the Rho1D4 agarose plus the Rho1D4 peptide for elution. Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.

Proteins tagged with the epitope sequence of the Rho1D4 antibody can be purified with this affinity matrix. Rho1D4 peptide in the Elution Buffer serves to competitively bind to the affinity resin and release the tagged protein, providing gentler elution conditions than, for example, changing pH. Genaxon bioscience offers conveniently aliquoted Rho1D4 peptide that has been tested with Rho1D4 Agarose. Based on BioWorks Workbeads, this product is the first commercially available immunoaffinity resin for the Rho1D4 purification system. Properties: - demonstrably suitable for membrane protein research - binding capacity of 3-4mg protein per mL resin - purifies protein with high specificity - gentle protein elution based on competitive binding - can be regenerated for reuse. Genaxxon resins are produced under strict quality guidelines and each batch undergoes quality checks to ensure that the loaded matrix has a high protein capacity. Combined with the specificity of the antibody-epitope interaction, a purification protocol optimized for the target protein can generate elution fractions with exceptionally high yields. This set offers both the Rho1D4 agarose plus the Rho1D4 peptide for elution. Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.

Rho1D4 peptide in the Elution Buffer serves to competitively bind to the affinity resin and release the tagged protein, providing gentler elution conditions than, for example, changing pH. Genaxxon offers conveniently aliquoted Rho1D4 peptide that has been tested with our Rho1D4 Agarose. The peptide can be purchased individually, or together with Genaxxon Rho1D4 Agarose as part of a Starter Set. 5mg peptide is sufficient for 1mL Rho1D4 Agarose or MagBeads. Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.

Rho1D4 antibody (antibody to Rhodopsin) is available as a purified mouse monoclonal antibody. The Rho1D4 specifically binds to the C- terminal epitope -T-E-T-S-Q-V-A-P-A- (COOH) of rhodopsin. This epitope can be engineered onto the C-terminus of proteins by recombinant techniques for use in - immunocytochemical analysis of proteins- co-immunoprecipitation- purification of epitope-tagged proteins for structure-function studies- related affinity-based techniques Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.

Glutathione MagBeads are developed for the affinity purification of glutathione-S-transferase (GST) fusion proteins. The affinity matrix is based on spherical magnetic cross-linked agarose. The material is highly porous to allow optimal protein interaction. Cross-linked agarose is also physically very stable, making it suitable for purification processes without deformation or destruction. Our magnetic beads are very homogeneous in size with a medium particle diameter of 30µm, yielding a high degree of reproducibility between individual purification runs.Glutathione is coupled to the magnetic agarose beads to obtain an affinity matrix with highest binding capacity for GST fusion proteins. Because the purification method depends on correctly folded GST protein, only native conditions can be used.Glutathione MagBeads are delivered as a 25% suspension in 20% ethanol. Therefore, 1mL suspension will yield a 250µL bed volume. The suspension contains 20% ethanol to prevent microbial growth. Genaxxon MagBeads have a binding capacity of >10mg protein/mL settled beads. Protein Binding CapacityThe protein binding capacity is up to 10mg/mL settled beads, as determined by purification of glutathione-S-transferase (GST) from E.coli cleared lysates, and quantified via spectrophotometry. GST-tagged proteins expressed in bacteria, yeasts, insects and in cell culture supernatants can be readily purified in a single purification step even from proteins expressed at low levels. After binding of target molecules, the magnetic resin can be readily isolated with the aid of a magnet. The GST tag can be cleaved in bound condition or in eluted condition by specific proteases such as TEV-express. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. Genaxxon MagBeads are ferrimagnetic agarose beads coupled to glutathione, an efficient ligand for GST fusion proteins. Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.

The GST tag is a 26 kDa protein that binds to glutathione-coupled agarose resins. This tag is considerably larger than the His tag and is often chosen to promote recombinant protein folding. In a comparative study with equivalent, market-leading resins, Genaxxon's Glutathione Agarose proved to be a competitive alternative, exhibiting the same protein binding capacity. Compared to competitor G both resins yielded up to 10mg/mL protein. Protein binding capacity: up to 10mg/mL. Compatible with a range of detergents and additives.

The Medicago Protein L-Ligand Leakage Elisa kit has been designed to detect and quantify Protein L in Immunoglobulin (Ig) or Ig-fragment containing solutions, e.g. eluates from Protein L affinity chromatography. The kit was developed as a complement to Cytiva’s affinity chromatography medium Capto L.Easy, stepwise procedureThe Protein L Ligand Leakage Elisa kit is easy to use. It contains all the components necessary for your daily lab routine and is based on a simple stepwise procedure. The concentration of Protein L in your samples is calculated and presented as a calibration curve indicating any ligand leakage in your eluates.Verification of pharmaceutical processesThe kit is for verifying affinity chromatography process quality in purification of pharmaceuticals using chromatography. A low leakage level of Protein L in the column used is proof that both the medium and the process as such are performing as expected. Compared to developing your own protocols, using this validated detection kit is an easy and secure way for researchers and manufacturers to continuously check for leakage and to ensure process quality.The kit comes as a sandwich ELISA with microtiter strips coated with an affinity purified anti-Protein L IgY-antibody. In this assay, samples are boiled to separate the Ig/Ig-fragments from the Protein L in the solution, centrifuged, and incubated in the strip wells. The bound Protein L is then detected by adding a horseradish peroxidase (HRP)-conjugated anti-Protein L IgY antibody. Substrate is added to induce a color change which is proportional to the amount of bound Protein L from the sample. The color change is quantified spectrophotometrically at 450nm by using a plate reader. The amount of Protein L in the sample is determined by using a standard curve prepared in a reference sample. The reference sample should have the same concentration of Ig or Ig-fragments and preferably the same buffer composition as the elution samples from the Protein L affinity chromatography medium. However, it should be prepared using another purification strategy, e.g. ion exchange chromatography. Once a reference sample is created it can be aliquoted and used for several different protein L ELISA assays. ApplicationsDetermination of leakage levels of Protein L in Protein L-medium purification of intact immunoglobulin or immunoglobulin fragments (Ig or Ig fragments). Some fab samples may require optimization. Currently there is no protocol available for sdab molecules. Shipping and storageThe product is shipped cooled (+2°C to +8°C) except the Protein L reference which is shipped frozen (-20°C). On arrival store components cooled ( +2°C to +8°C ) except the Protein L reference which should be stored at -20/-80°C.Strips (96 wells): Store unused strips in sealed bag at +2°C to +8°C . HRP-conjugated anti Protein L pAb and EC-Blue Enhanced TMB-substrate: Store protected from light. Protein L reference: Store in working aliquots to avoid repeated freezing/thawing.

The Medicago Protein L-Ligand Leakage Elisa kit has been designed to detect and quantify Protein L in Immunoglobulin (Ig) or Ig-fragment containing solutions, e.g. eluates from Protein L affinity chromatography. The kit was developed as a complement to Cytiva’s affinity chromatography medium Capto L.Easy, stepwise procedureThe Protein L Ligand Leakage Elisa kit is easy to use. It contains all the components necessary for your daily lab routine and is based on a simple stepwise procedure. The concentration of Protein L in your samples is calculated and presented as a calibration curve indicating any ligand leakage in your eluates.Verification of pharmaceutical processesThe kit is for verifying affinity chromatography process quality in purification of pharmaceuticals using chromatography. A low leakage level of Protein L in the column used is proof that both the medium and the process as such are performing as expected. Compared to developing your own protocols, using this validated detection kit is an easy and secure way for researchers and manufacturers to continuously check for leakage and to ensure process quality.The kit comes as a sandwich ELISA with microtiter strips coated with an affinity purified anti-Protein L IgY-antibody. In this assay, samples are boiled to separate the Ig/Ig-fragments from the Protein L in the solution, centrifuged, and incubated in the strip wells. The bound Protein L is then detected by adding a horseradish peroxidase (HRP)-conjugated anti-Protein L IgY antibody. Substrate is added to induce a color change which is proportional to the amount of bound Protein L from the sample. The color change is quantified spectrophotometrically at 450nm by using a plate reader. The amount of Protein L in the sample is determined by using a standard curve prepared in a reference sample. The reference sample should have the same concentration of Ig or Ig-fragments and preferably the same buffer composition as the elution samples from the Protein L affinity chromatography medium. However, it should be prepared using another purification strategy, e.g. ion exchange chromatography. Once a reference sample is created it can be aliquoted and used for several different protein L ELISA assays. ApplicationsDetermination of leakage levels of Protein L in Protein L-medium purification of intact immunoglobulin or immunoglobulin fragments (Ig or Ig fragments). Some fab samples may require optimization. Currently there is no protocol available for sdab molecules. Shipping and storageThe product is shipped cooled (+2°C to +8°C) except the Protein L reference which is shipped frozen (-20°C). On arrival store components cooled ( +2°C to +8°C ) except the Protein L reference which should be stored at -20/-80°C.Strips (96 wells): Store unused strips in sealed bag at +2°C to +8°C . HRP-conjugated anti Protein L pAb and EC-Blue Enhanced TMB-substrate: Store protected from light. Protein L reference: Store in working aliquots to avoid repeated freezing/thawing.