FAQs about SNP Pol DNA Polymerase

Can I use SNP Pol DNA Polymerase with bisulfite-treated DNA samples?

Yes, you can. Our SNP Pol DNA Polymerase > is for example performing very well in methylation specific PCR (MSP). Since a single mismatch is sufficient for specific MSP results, SNP Pol DNA Polymerase even enables reliable MSP analysis of single CpG sites.

Can I use SNP Pol 2x PCR Master Mix and SNP Pol DNA Polymerase in probe-based assays?

Yes, you can use it in probe assays, which don't require a nuclease activity (e.g., HybProbe or SimpleProbe). Please be aware that SNP Pol DNA Polymerase doesn't have a 5'-3'-nuclease activity, so you can't use SNP Pol 2x PCR Master Mix > in hydrolysis-based probe assays (e.g., TaqMan®). For this assays, we recommend to use our SNP PolTaq DNA Polymerase > with 5'-3'-nuclease activity. SNP PolTaq variant can therefore be used for hydrolysis probe-based real-time PCRs.

What is the error-rate of SNP Pol DNA Polymerase?

The error-rate of SNP Pol is comparable to the error-rate of wildtype Taq DNA Polymerase.

Can I use SNP Pol DNA Polymerase in real-time PCRs using a real-time dye such as SYBR Green?

Please be aware that SNP PolTaq DNA Polymerase is not suitable for real-time PCRs using a real-time dye. SNP Pol DNA Polymerase works very well with real-time dyes.

For which amplicon length should primers be designed?

We recommend designing primers with a short amplicon length (about 60-200 bp) for best results, but also longer amplicon lengths are possible.