Exosome Maxi Purification Kit - Cell Culture Medium, Urine, liquid samples
Vorteile im Überblick
- Versatile Sample Compatibility: Ideal for isolating exosomes from cell culture medium.
- Purity and Integrity: Ensures high-quality exosomes suitable for cell culture applications and animal injections.
- Functional Analysis: Perfect for isolating EVs for in-depth functional analysis of exosomes.
- Efficient Procedure: The isolation process is rapid and consistent, providing reliable results every time.
Produktnummer:
S5326.0010
Lieferzeit: 3 - 8 Werktage
Für genaue Informationen zum Liefertermin wenden Sie sich bitte an Genaxxon.
Shipment: not cooled. Store at +2°C to +8°C. For laboratory usage only!
Lieferzeit: 3 - 8 Werktage
Für genaue Informationen zum Liefertermin wenden Sie sich bitte an Genaxxon.
Shipment: not cooled. Store at +2°C to +8°C. For laboratory usage only!
Produktinformationen "Exosome Maxi Purification Kit - Cell Culture Medium, Urine, liquid samples"
Genaxxon’s Exosome Maxi Purification Kit ensures high efficiency in exosome and extracellular vesicle (EV) isolation.
Designed to purify exosomes from cell culture medium, this kit leverages advanced magnetic bead technology. Achieve results comparable to or better than ultracentrifugation, without the need for specialized equipment.
The kit efficiently isolates exosomes using magnetic beads that adsorb exosomes during purification and release them in the elution buffer, ensuring high purity and yield. These eluted exosomes are ideal for cell culture applications and animal injections and are suitable for immediate functional analyses such as NTA.
Applications
The Genaxxon Serum Exosome Purification Kit is versatile and can be used to isolate microvesicles from cell culture medium.
The isolated vesicles are perfect for a range of analytical and functional applications, including:
- Physical Characterization: Techniques such as Nanoparticle Tracking Analysis (NTA) and Tunable Resistive Pulse Sensing (TRPS)
- Electron Microscopy
- Isolation and Analysis: Suitable for DNA, RNA, proteins, lipids, and other constituents
- Flow Cytometry
- Antibody- or Fluorescent Dye-Based Labeling
- Uptake by Recipient Cells
Choose the Genaxxon Exosome Maxi Purification Kit for reliable, high-quality exosome isolation, enabling advanced research and application in various biological fields.
FAQs
Keine Suchergebnisse vorhanden.
Low Yield of exosomes can have different reasons of which some are listed here:
Exosome amount or concentration: Use samples contain more exosomes, e.g. concentrate samples or increase sample volume if possible.
Storage: Exosomes are very susceptible to repeated freeze-thaw cycles or to high storage temperatues. Always keep exosomes at -80°C for long term storage and do not allow repeated freeze-thaw cycles.
Kit related issues: Always centrifuge your sample at 16000xg before filtering it to get rid of the cell debris and to avoid clogging the filter membrane in the next step. Filter the centrifuged sample through a filter with a pore size between 0.8µm and 0.2µm.
If you use magnetic beads for further purification, the proportion of reagent volume is crucial for optimal efficiency. For this do not use less beads (less bead volume) than recommended and always use the recommended amount of reagent for incubation and washing. Incubation mode and elution intensity should be carried out in strict accordance with the instructions.
Sometimes the incubation time between samples and magnetic beads is too short. In such a case a prolonged incubation time can increase the exosome amount isolated as can an increased vortex intensity and vortex time.
Exosomes are quite fragile and can be easily damaged or altered by harsh mechanical or chemical processes found in many commercial exosome isolation kits. This can negatively impact downstream applications such as Next-Generation Sequencing (NGS).
Here are steps you can take to minimize damage and maintain the integrity of purified exosomes:
- Use Gentle Isolation Conditions: Employ low temperatures and mild centrifugal forces during the isolation process. Avoid high-speed centrifugation steps that could cause exosome rupture.
- Optimize Buffers: Use buffers that protect the structural integrity of exosomes, such as phosphate-buffered saline (PBS) or Tris-buffered saline (TBS). Ensure the pH and osmolarity of the buffers are suitable for exosome stability.
- Proper Storage Conditions: Store exosomes at low temperatures, preferably -80°C, to prevent degradation. Avoid repeated freeze-thaw cycles as they can damage the exosomal membrane.
- Quality Control Assessments: Regularly perform quality control assessments such as transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and Western blotting. These assessments help check the morphology, size distribution, and protein markers of the exosomes to ensure their integrity.
Different isolation methods have varying capabilities to remove contaminants. For instance, immunocapture can use specific antibodies to bind exosomes, thereby reducing contamination from other components. Size exclusion chromatography (SEC) can also effectively separate exosomes from smaller particles.
To improve the overall purity of exosomes a Multi-Step Purification (combining different purification methods) method can be applied. E.g. using ultracentrifugation followed by density gradient
centrifugation and size exclusion chromatography can effectively remove various types of contaminants.
Contamination from microorganisms can compromise the quality of your samples. To prevent and address this issue, follow these guidelines:
By adhering to these practices, you can minimize the risk of microbial contamination and maintain the quality of your samples.
- Strict Protocol Adherence: Maintain a clean work environment and use sterile techniques to avoid cross-contamination during the process. Use disposable centrifuge tubes and filters, and ensure all equipment is cleaned and calibrated regularly.
- Using the Right Filter Membrane: Ensure that the final filtration step is carried out in a clean bench. Use filter membranes with pore sizes no larger than 0.22 µm to effectively remove microorganisms.
- Using Specialized Kits and Reagents: Utilize specialized kits and reagents that are designed to address contamination issues. These products simplify the process and significantly reduce the chances of contamination.
Exosomes are known as cell-secreted natural nanovesicles, and the low-yield of exosome production from MSC-conditioned medium under conventional culture conditions limits their applications. The optimal cell culture medium can enhance exosome secretion from MSCs.
For exosome secretion from adipose-derived stem cells, we recommend culturing ADSCs in dishes or flasks under regular culture conditions up to 90% confluence. Then remove the original culture medium and wash the cells with PBS and our ADSC Exosome HY medium (C4161) to remove residues of the original medium. Then the ADSC cells are cultured in our exosome enrichment medium ADSC Exosome HY medium for 3-4 days before the conditioned medium can be used for exosome isolation.
For exosome purification, we recommend starting by preparing a conditioned medium after cell culture (e.g. using our ADSC Exosome HY Medium. The supernatant is then collected from cultured cells, and large fragments of cell debris, waste products produced by cell metabolism, as well as impurities in the culture medium are removed through any exosome isolation method (e.g. using our exosome isolation kits).
In general, commonly used supplements like serum or human platelet lysate contain exosomes. Therefore, we do not recommend using supplements in the preparation of conditioned medium for exosome isolation.
When producing exosomes using our ADSC eXOrich medium, there is no need to add common nutritional supplements like serum or human platelet lysate. However, users can decide to add appropriate nutritional supplements based on their specific needs.
magnetic bead technology for isolation of exosomes and other VEs
Sample size: up to 14.65mL
Applikation / Application:
Nanoparticle Tracking Analysis (NTA), Tunable Resistive Pulse Sensing (TRPS), Electron Microscopy, Flow Cytometry, Antibody- or Fluorescent Dye-Based Labeling, Uptake by Recipient Cells
Einheiten / Units:
Quelle / Source:
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-04-05
Dokumente - Protokolle - Downloads :
Hier finden Sie Informationen und weiterführende Literatur. Für weitere Dokumente (Zertifikate mit weiteren Lotnummern, Sicherheitsdatenblätter in anderer Sprache, weitere Produktinformationen) wenden Sie sich bitte an Genaxxon biosience unter: info@genaxxon.com oder Tel.: +49 731 3608 123.
Dokumente:
Produktbeschreibung
Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Quelle: NCBI PubMed
Quelle: NCBI PubMed
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