FAST DNA polymerase
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Lieferzeit 20 Tage
Shipment: on wet ice. Store at -20°C. For laboratory usage only!
Genaxxon's FAST DNA polymerase is a robust enzyme, ideally suited for applications like genotyping and screening, amplifying with extreme speed of up to 2 seconds per 1kb, as well as high yield and consistency. FAST DNA polymerase has 5’-3’ exonuclease activity, but no 3’-5’ exonuclease (proofreading) activity. PCR products generated with this enzyme are A-tailed and can thus be cloned into TA cloning vectors. The extreme speed of Genaxxons' FAST DNA polymerase allows the use of an extension rate of 4-8 kb/min. Genaxxon's FAST DNA Polymerase is delivered with 5X reaction buffer already containing MgCL2, dNTPs, enhancers and stabilizers for an optimal PCR result.
Genaxxon's FAST DNA polymerase is also available as a convenient 2x master mix (M3286), including an inert red tracking dye for electrophoresis, containing all required components for fast PCR, except specific primers.
Specifications:
Concentration: 5 units/µL
Extension rate: up to 30 kb/min. at 72°C
5’-3’ exonuclease activity: Yes
Extra addition of A: Yes
3’-5’ exonuclease activity: No
Nuclease contamination: No
Protease contamination: No
RNase contamination: No
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02
Dokumente:
DatenblätterQuelle: NCBI PubMed
2 seconds extention time for targets below 1 kb and 15 seconds per kb for target DNA up to 5kb at 72ºC for extension.