FAST DNA polymerase

Genaxxon's FAST DNA polymerase is a robust enzyme, ideally suited for applications like genotyping and screening, amplifying with extreme speed of up to 2 seconds per 1kb, as well as high yield and consistency. FAST DNA polymerase has 5’-3’ exonuclease activity, but no 3’-5’ exonuclease (proofreading) activity. PCR products generated with this enzyme are A-tailed and can thus be cloned into TA cloning vectors. The extreme speed of Genaxxons' FAST DNA polymerase allows the use of an extension rate of 4-8 kb/min. Genaxxon's FAST DNA Polymerase is delivered with 5X reaction buffer already containing MgCL2, dNTPs, enhancers and stabilizers for an optimal PCR result.

Genaxxon's FAST DNA polymerase is also available as a convenient 2x master mix (M3286), including an inert red tracking dye for electrophoresis, containing all required components for fast PCR, except specific primers. 

Example of a typical PCR protocol (graphical presentation)

* Ta is the annealing temperature (Ta should be 2°C above Tm**)

 ** Tm is the melting temperature of a primer which is defined as the temperature at which 50% of the primer bind to the complementary sequence of the target DNA. Please note: By increasing Ta above Tm, this percentage decreases, however, primers will still anneal up to a certain degree and initiate extension. Therefore, PCR would still work with a Ta of several degrees higher than the Tm but with a dramatically reduced efficiency. Hence, we recommend optimizing the Ta by performing a temperature gradient (e.g., starting at the lowest Tm or a few degrees below and increasing with 2°C increments). 

*** Largest tested amplicon size: 5kb. Please note that longer extension times may be required for targets larger than 5kb!