FAST HotStart DNA polymerase

Genaxxon’s FAST HotStart DNA polymerase is a robust enzyme with an antibody mediated hotstart function, ideally suited for applications like genotyping and screening, amplifying with extreme speed of up to 2 seconds per 1kb, as well as high yield and consistency. 

PCR products generated with this enzyme are A-tailed and can thus be cloned into TA cloning vectors. The extreme speed of Genaxxon’s FAST HotStart DNA polymerase allows the use of an extension rate of 4-8 kb/min. The buffer composition has been optimized via high-throughput screening and makes the enzyme particularly resistant to PCR inhibitors. Therefore, Genaxxon’s FAST HotStart DNA polymerase is suitable for direct PCR from unpurified samples, including blood, urine, and bacterial colonies, making this the ideal choice for consistent results in fast complex PCR amplifications.

Genaxxon's FAST HotStart DNA polymerase is also available as a convenient 2x master mix (M3288), including an inert red tracking dye for electrophoresis, containing all required components for fast PCR, except specific primers. 

Example of a typical PCR protocol (graphical presentation)

* Ta is the annealing temperature (Ta should be 2°C above Tm**)

 ** Tm is the melting temperature of a primer which is defined as the temperature at which 50% of the primer bind to the complementary sequence of the target DNA. Please note: By increasing Ta above Tm, this percentage decreases, however, primers will still anneal up to a certain degree and initiate extension. Therefore, PCR would still work with a Ta of several degrees higher than the Tm but with a dramatically reduced efficiency. Hence, we recommend optimizing the Ta by performing a temperature gradient (e.g., starting at the lowest Tm or a few degrees below and increasing with 2°C increments). 

*** Largest tested amplicon size: 5kb. Please note that longer extension times may be required for targets larger than 5kb!