TEMPase Hot Start DNA-Polymerase
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Lieferzeit: 3 - 8 Werktage
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Shipment: on wet ice. Store at -20°C. For laboratory usage only!
About TEMPase Hot Start DNA Polymerase
TEMPase Hot Start DNA Polymerase has been designed to avoid spurious amplification and to diminish the formation of non specific priming events during your set up. TEMPase Hot Start DNA Polymerase allows for detection of low copy number targets and for multiplex PCR in a single reaction tube. TEMPase Hot Start DNA Polymerase is available together with different buffers. This one (A2231xx) is supplied with 10X combination buffer plus separate 25mM MgCl2.
TEMPase Hot Start DNA Polymerase is also available together with 10X ammonium sulfate buffer and separate 25mM MgCl2 (A2211xx >).
Features
- Reaction set up at room temperature
- Increased sensitivity
- Increased specificity
- Increased product yield
Description
TEMPase Hot Start DNA Polymerase is a modified form of Ampliqon Taq DNA Polymerase, which is activated by heat treatment.
A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during set up and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended.
Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a heat activation step, releasing the active TEMPase Hot Start DNA Polymerase into the reaction.
- Beachten Sie auch den Genaxxon SuperHot Hot Start Mastermix > mit und ohne Farbstoff.
Mit unseren hochwertigen dNTPs als Set (M3015.4100 und M3015.0250) > oder als Mix (M3016.1010) > oder unseren DNA-Markern > und unserer günstigen Standardagarose > bieten wir Ihnen weitere Produkte für die PCR.
Specifications:
5 units/µL.
shipped with 10X combination buffer plus separate 25mM MgCl2.
Enyzme is completely inactive prior to heating at 95°C for 15 min.!
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02
Dokumente:
DatenblätterSpez. Produktbeschreibung
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Kategorienliste
Quelle: NCBI PubMed
