FAST HotStart DNA polymerase (2X) MasterMix with dye
Vorteile im Überblick
- Very high speed (2 sec/kb)
- Offering high yields and consistency
- Ideal for daily applications (genotyping, screening)
- Antibody mediated hot start
Sofort versandfertig, Lieferzeit 1-2 Werktage
Shipment: on wet ice. Store at -20°C. For laboratory usage only!
Genaxxon’s FAST HotStart DNA polymerase (2X) MasterMix with dye is the master mix of our FAST HotStart DNA polymerase (M3287) which is a robust enzyme, ideally suited for applications like genotyping and screening, amplifying with extreme speed of up to 2 seconds per 1kb, as well as high yield and consistency.
PCR products generated with this enzyme are A-tailed and can thus be cloned into TA cloning vectors.
The extreme speed of Genaxxon’s FAST HotStart DNA polymerase allows the use of an extension rate of 4-8 kb/min. The buffer composition has been optimized via high-throughput screening and makes the enzyme particularly resistant to PCR inhibitors. Due to its robustness, Genaxxon’s FAST HotStart DNA polymerase (2X) MasterMix with dye is suitable for direct PCR from unpurified samples, including blood, urine, and bacterial colonies, making it the ideal choice for consistent results in fast complex PCR amplifications. Besides this, it includes an inert red tracking dye for gel electrophoresis. Therefore, upon completion of the PCR, the reaction is ready for direct loading onto an agarose gel without the need of adding an additional loading buffer.
Example of a typical PCR protocol (graphical presentation)
* Ta is the annealing temperature (Ta should be 2°C above Tm**)
** Tm is the melting temperature of a primer which is defined as the temperature at which 50% of the primer bind to the complementary sequence of the target DNA. Please note: By increasing Ta above Tm, this percentage decreases, however, primers will still anneal up to a certain degree and initiate extension. Therefore, PCR would still work with a Ta of several degrees higher than the Tm but with a dramatically reduced efficiency. Hence, we recommend optimizing the Ta by performing a temperature gradient (e.g., starting at the lowest Tm or a few degrees below and increasing with 2°C increments).
*** Largest tested amplicon size: 5kb. Please note that longer extension times may be required for targets larger than 5kb!
Specifications:
Genaxxon’s FAST HotStart DNA polymerase (2X) MasterMix with dye is supplied as a convenient 2x master mix, including an inert red tracking dye for electrophoresis, containing all required components for fast PCR, except specific primers. Final concentration of MgCl2 will be 3mM. One mL is suitable for 80 reactions of 25µL (or 100 reactions of 20µL).
Extension rate: up to 30 kb/min. at 72°C
Hotstart function: Yes
5’-3’ exonuclease activity: Yes
Extra addition of A: Yes
3’-5’ exonuclease activity: No
Nuclease contamination: No
Protease contamination: No
RNase contamination: No
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02
Dokumente:
DatenblätterKategorienliste
Quelle: NCBI PubMed
2 seconds extention time for targets below 1 kb and 15 seconds per kb for target DNA up to 5kb at 72ºC for extension.
