FAST HotStart DNA polymerase (2X) MasterMix with dye
Vorteile im Überblick
- Hochwertige Produkte
- Kundenspezifische Lösungen
- Persönlicher Kontakt
- Schneller Service
- Kompetenter technischer Support +49(0)731-3608-123
Sofort versandfertig, Lieferzeit 1-2 Werktage
Shipment: on wet ice. Store at -20°C. For laboratory usage only!
Genaxxon’s FAST HotStart DNA polymerase (2X) MasterMix with dye is the master mix of our FAST HotStart DNA polymerase (M3287) which is a robust enzyme, ideally suited for applications like genotyping and screening, amplifying with extreme speed of up to 2 seconds per 1kb, as well as high yield and consistency. PCR products generated with this enzyme are A-tailed and can thus be cloned into TA cloning vectors. The extreme speed of Genaxxon’s FAST HotStart DNA polymerase allows the use of an extension rate of 4-8 kb/min. The buffer composition has been optimized via high-throughput screening and makes the enzyme particularly resistant to PCR inhibitors. Therefore, Genaxxon’s FAST HotStart DNA polymerase (2X) MasterMix with dye is suitable for direct PCR from unpurified samples, including blood, urine, and bacterial colonies, making this the ideal choice for consistent results in fast complex PCR amplifications. Besides this, it includes an inert red tracking dye for gel electrophoresis. Therefore, upon completion of the PCR, the reaction is ready for direct loading onto an agarose gel without the need of adding an additional loading buffer.
Specifications:
Genaxxon’s FAST HotStart DNA polymerase (2X) MasterMix with dye is supplied as a convenient 2x master mix, including an inert red tracking dye for electrophoresis, containing all required components for fast PCR, except specific primers. Final concentration of MgCl2 will be 3mM. One mL is suitable for 80 reactions of 25µL (or 100 reactions of 20µL).
Extension rate: up to 30 kb/min. at 72°C
Hotstart function: Yes
5’-3’ exonuclease activity: Yes
Extra addition of A: Yes
3’-5’ exonuclease activity: No
Nuclease contamination: No
Protease contamination: No
RNase contamination: No
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02
Dokumente:
DatenblätterQuelle: NCBI PubMed
2 seconds extention time for targets below 1 kb and 15 seconds per kb for target DNA up to 5kb at 72ºC for extension.