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RealQ Plus 2x Master Mix Green - Low ROX

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  • Hochwertige Produkte
  • Kundenspezifische Lösungen
  • Persönlicher Kontakt
  • Schneller Service
  • Kompetenter technischer Support +49(0)731-3608-123

1.695,00 €*

Volumen
Produktnummer: A324406
Lieferzeit: 3 - 8 Werktage
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Shipped on wet ice, store at -20°C. For laboratory usage only!
Produktinformationen "RealQ Plus 2x Master Mix Green - Low ROX"

Introduction:

Quantitative PCR is an important tool for SNP and gene expression analysis. Two general fluorescent chemistries exist for quantitative detection of gene transcripts: probes (e.g. TaqMan®, Scorpions™ Probes, molecular beacons) and DNA-binding fluorescent dyes (e.g. ethidium bromide, SYBR® Green, EvaGreen®, PicoGreen®). Ampliqon offers the RealQ Plus 2x Master Mix in two formulations: for probe or with DNA-binding fluorescent dye, making them ideal for most quantitative PCR applications.

The RealQ Plus 2x Master Mixes are available with high, low or without ROX™ for optimal performance on most of the commonly used real-time PCR instruments. The RealQ Plus 2x Master Mixes promote high specificity and low background by using TEMPase Hot Start DNA Polymerase, a modified Taq DNA polymerase with hot start capabilities.

The RealQ Plus 2x Master Mix Green with low ROX™ is a singletube 2x reagent including all components necessary to perform real-time DNA amplification for DNA-binding dye based PCR. Just add your primers and DNA. The ROX™ internal reference dye level is optimized for real-time instruments that require low ROX™ as internal reference dye e.g. the Stratagene Mx3005P.

Detection limit: approximately 1 copy.

Quantitation limit: approximately 24 copies (0.08ng of human gDNA, correlating to 12 diploid genomes, with 2 gene copies per diploid genome)

Compatibility: Real-time instruments which require low ROX™ as internal reference dye e.g. the Stratagene Mx3005P.

Specifications:
The ProbeMastermix is a ready-to-use 2-time mastermix that contains a green fluorescence dye, dUTP instead of dTTP and 50nM ROX™ (low ROX concentration) as reference dye.


Applikation / Application:
Low-copy targets PCR Real-time PCR Quantitative PCR

Einheiten / Units:
Quelle / Source:


Sicherheits Hinweise / Safety


Klassifizierungen / Classification

eclass-Nr: 32-16-05-02
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Quelle: NCBI PubMed