RNase Inhibitor (RNAsin) - Glycerinfrei

Vorteile im Überblick

Glycerol free - lyo-ready
200 units/µL
Inhibits common RNases (RNase A, RNase B, RNase C and human placental RNase)
Does not inhibit RNase 1, RNase T1, RNase T2, S1 nuclease, and RNase H.
Compatible with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases.
Tested for the absence of DNases and RNases

128,75 €

Units

2000 units

Produktnummer: M3169.2000

Lieferzeit: 14 days

Unsere Vorteile

Kostenloser Versand
Versand innerhalb von 24h
30 Tage Geld-Zurück-Garantie
Bequemer Kauf auf Rechnung

Lieferzeit: 3-8 Tage

Shipment: on wet ice. Store at -20°C. For laboratory usage only!

Produktinformationen "RNase Inhibitor (RNAsin) - Glycerinfrei"

The Genaxxon RNase Inhibitor is a recombinant (E.coli) murine protein which inhibits ribonucleases (RNases) A, B and C. It does not inhibit RNase 1, RNase H, RNase T1, S1 Nuclease. Native RNase-Inhibitor from murine placenta exerts its inhibitory effect by binding non-covalently to RNases in a 1:1 ratio with high affinity.

There is no inhibition of polymerase activity when this RNase Inhibitor is used with AMV Reverse Transcriptase, M-MuLV Reverse Transcriptase, Taq DNA Polymerase or Phage RNA Polymerase (SP6, T7, or T3).

The enzyme is active over a broad pH range between 5 and 9, with a maximum activity at pH7 – pH8.

The inhibition of RNases is a reversible process. Reagents as urea or thiol reagents will lead to the dissociation of the Inhibitor-RNase-complex, resulting in re-natured and functional RNase and irreversibly deactivated RNase inhibitor.

Applications:

RT-PCR
cDNA synthesis
In vitro transcription/translation
Enzymatic RNA labelling reactions
In vitro virus detection.
Improvement of RNA translation in homologous systems.
Preparations of RNase-free antibodies.
Any application where eukaryotic RNase contamination is a potential problem.

Advantages:

The recombinant murine RNase inhibitor does not contain the cysteine pair that was identified to cause the high sensitivity against oxidation resulting in the inactivation of the human RNase inhibitor (1). As a result, the murine RNase inhibitor has significantly improved oxidation resistance compared to the human and porcine RNase inhibitors. In addition, murine RNase inhibitor is much more stable at low low DTT concentrations (less than 1 mM), what makes it ideal for reactions where high DTT concentrations interfere with the reaction (e.g. real-time RT-PCR).

Product does not contain glycerol => lyo-ready product (ready for preparations of freeze-dried products).

Recommendations:

Since the RNase inhibitor protein is sensitive to denaturing agents and conditions, whereas RNases are not, care must be taken to avoid high concentrations of urea or other denaturing agents and temperatures above 50-55°C.

The recommended concentration of the RNase inhibitor is 1 unit/µL.

During set-up of a reaction, RNase inhibitor should be added before other components are added (enzymes, plasmids from mini preps, etc.).

References

1. Kim, B.M. et al. Protein Science. 8, 430-434.

Specifcations:
200 units/µL
Glycerol free
Recombinant protein of murine origin
Inhibits common eukaryotic RNases (RNase A, RNase B, RNase C and human placental RNase)
Does not inhibit RNase 1, RNase T1, RNase T2, S1 nuclease, and RNase H.
Compatible with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases.
Active over a broad pH range (pH 5-8).
Tested for the absence of DNases and RNases

Applikation / Application:

- Reversible inhibition of RNase- Suitable for all applications with RNA, especially for reverse transcription of RNA into cDNA- Useful if eukaryotic RNase contamination occurs

Einheiten / Units:

one unit of RNase inhibitor is defined as the amount of protein required to inhibit 50% of the activity of 5ng of RNase A

Quelle / Source:

E. coli strain that carries the murine Ribonuclease Inhibitor gene

Klassifizierungen / Classification

eclass-Nr: 32-16-04-10

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