Artikel-Nr.: P2005.0020Shipping: shipped on wet ice, stored at -20°C
Lieferzeit: normalerweise 3 - 8 Werktage
(Bei Spezialzellkulturmedien: produktionsbedingt ca. 4 Wochen - Bitte wenden Sie sich an Genaxxon.)
esiRNA negative controls:
- RLUC (Renilla Luciferase)
- FLUC (Firefly Luciferase)
- EGFP (enhanced green-fluorescent protein)
Transfection with these esiRNAs should have no effect, if the corresponding target is not expressed by the cells. Therefore, these esiRNAs are ideal to optimize the concentration of transfection reagent for minimal cell toxicity.
All esiRNAs targeting protein-coding genes were designed based on the sequence annotations from the ENSEMBL database.
Quality Control: esiRNAs are designed using the DEQOR design algorithm. The PCR products used for production are tested via gel electrophoresis, and identity is confirmed by DNA sequencing. Quality of esiRNA is validated by gel electrophoresis and concentrations are determined by measuring OD260 or fluorescence intensity.
Purification: RNaseIII-digested products are purified by anion-exchange chromatography, precipitated with isopropanol and washed with ethanol.
Format: All esiRNA are delivered in solution (resuspended in nuclease-free TE buffer) normalized to 200ng/µL.
Storage and Stability: esiRNAs stored at -20°C are stable for 2 years. No decrease in stability was observed after 12 freeze/thaw cycles.
Technical Data: esiRNA will be delivered with a technical data sheet, which includes the esiRNA ID number, organism, amount (µg), concentration, lot #, and target sequence.
RNaseIII-digested products purified by anion-exchange chromatography, precipitated with isopropanol and washed with ethanol. Resuspended in nuclease-free TE buffer and normalized to 200ng/µL.
esiRNAs stored at -20°C are stable for 2 years. No decrease in stability was observed after 12 freeze/thaw cycles.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 25-09-90-90
Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
In vivo topology converts competition for cell-matrix adhesion into directional migration
Fernanda Bajanca, Nadège Gouignard, Charlotte Colle, Maddy Parsons, Roberto Mayor, Eric Theveneau
Nat Commun. 2019; 10: 1518. Published online 2019 Apr 3. doi: 10.1038/s41467-019-09548-5