Cas9-NLS (CRISPR associated protein 9)

Cas9-NLS (CRISPR associated protein 9)


Artikel-Nr.: S5336.0010

Shipping: shipped on wet ice, stored at -20°C

Lieferzeit: normalerweise 3 - 8 Werktage - Bitte wenden Sie sich an Genaxxon

234,00 € *

Gewicht:

Wählen Sie bitte die gewünschte Packungsgröße aus.

The Genaxxon Cas9-NLS represents the wild-type Cas9 nuclease from S. pyogenes , fused with a... mehr
Produktinformationen "Cas9-NLS (CRISPR associated protein 9)"

The Genaxxon Cas9-NLS represents the wild-type Cas9 nuclease from S. pyogenes, fused with a C-terminal NLS. Currently, the best-established Cas9 protein in the field with most publications. Cas9-NLS (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes, among other bacteria. S. pyogenes utilizes Cas9 to remember and later probe and cleave foreign DNA, such as invading bacteriophage DNA or plasmid DNA. In the case that the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA. In addition to its original role in bacterial immunity, the Cas9 protein has been heavily employed as a genome engineering tool to induce site-directed double strand breaks in DNA. These disruptions may lead to gene inactivation or the presentation of heterologous genes via non-homologous end joining and homologous recombination respectively in many laboratory model organisms.

Recombinant Cas9-NLS comprises the entire Cas9 protein sequence and the nuclear localization sequence PKKKRKV (= NLS of Simian virus 40) at the C-terminus. The protein has an apparent molecular weight of 160kDa on SDS-PAGE.

Synonyms: CRISPR-associated endonuclease Cas9/Csn1, SpyCas9, cas9, csn1.

Andere Cas9-NLS-Proteine von Genaxxon: Cas9-NLS (Nuklease, Wildtyp) >Cas9-NLS-tagRFP (Nuklease mit rot fluoreszierendem Proteinmarker) >Cas9-Dead-NLS >Cas9-Dead-NLS-EGFP >.

Transfektionsreagenz für die Co-Transfektions RNA/Protein, speziell entwickelt für den Einsatz in CRISPR/Cas9-Systemen: CRISPRfect E >.

Weitere Artikel passend zu "Cas9-NLS (CRISPR associated protein 9)"
Unser Kommentar zu "Cas9-NLS (CRISPR associated protein 9)"
Specific genome targeting Labelling of target sites.
Purity: >97% (determined by SDS-PAGE). Recombinant Cas9-NLS comprises the entire Cas9... mehr
 

Technische Daten:

Purity: >97% (determined by SDS-PAGE).

Recombinant Cas9-NLS comprises the entire Cas9 protein sequence and the nuclear localization sequence PKKKRKV (= NLS of Simian virus 40) at the C-terminus. The protein has an apparent molecular weight of 160kDa on SDS-PAGE.

Applikation:

CRISPR Cas9 Cloning

Quelle

E.coli

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

Dokumente - Protokolle - Downloads mehr

Dokumente - Protokolle - Downloads

Hier finden Sie Infos und weiterführende Literatur zu Cas9-NLS (CRISPR associated protein 9)

 
 
 
Bewertungen lesen, schreiben und diskutieren... mehr
Kundenbewertungen für "Cas9-NLS (CRISPR associated protein 9)"
Bewertung schreiben
Bewertungen werden nach Überprüfung freigeschaltet.
Bitte geben Sie die Zeichenfolge in das nachfolgende Textfeld ein.

Die mit einem * markierten Felder sind Pflichtfelder.

Cas9-Dead-NLS Protein Cas9-Dead-NLS Protein
ab 234,00 € *
Cas9-Nickase-NLS Cas9-Nickase-NLS
ab 234,00 € *
Cas9-NLS-EGFP Cas9-NLS-EGFP
ab 258,00 € *
Cas9-Dead-NLS-EGFP Cas9-Dead-NLS-EGFP
ab 258,00 € *
esiCRISPR Kit-wt esiCRISPR Kit-wt
550,00 € *
TIPP!
Premium dNTPs at low prices dNTP-Set (Na-Salz) - 100 mM Lösung
ab 25,00 € * 50,00 € *
Taq Polymerase from Genaxxon Taq DNA Polymerase S (Hohe Genauigkeit)
ab 10,00 € * 25,00 € *
Gelextraktionskit Gelextraktionskit
ab 50,32 € *
TIPP!
TIPP!
Taq Polymerase from Genaxxon Taq DNA Polymerase E (Hohe Ausbeute)
ab 10,00 € * 15,00 € *
Phusion Polymerase from Genaxxon ExactRun proofreading Polymerase
ab 32,38 € * 64,75 € *