5-Carboxyfluorescein diacetate N-succinimidyl ester / 5-FAM DA SE
Artikel-Nr.: S5540.0025
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5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside the cells, 6-CFDA is hydrolyzed by intracellular esterases to 6-carboxyfluorescein. Reagent for continuously determining the intracellular pH in bacteria by fluorometry. Labeling human intervertebral disk cells in vitro for fluorescence microscopy.
Technische Daten:
Specifications:
Assay: min. 95% (HPLC)
Identity determined by: NMR
Soluble in: DMSO and DMF
Fluorescence: λex 492 nm; λem 517 nm in 0.1M Tris pH8.0 (after cleavage by esterase)
C29H19NO11
MW = 557.46 g/mol
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
CAS-Nr: 150206-05-6eclass-Nr: 32-16-04-05
