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Aminoallyl-dUTP (min. 98% by HPLC) (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as a 10mM aqueous solutions titrated to pH7.0 with NaOH.
Aminoallyl-dUTP can be enzymatically incorporated into DNA with reverse Transcriptases, Taq DNA polymerase, phi29 DNA Polymerase, Klenow Fragment, and DNA Polymerase I. The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.
- Can be incorporated into DNA using reverse transcriptases, Taq DNA Polymerase, Klenow Fragment. - The resulting aminoallyl-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten, resulting in a uniform and high density labeling of DNA.
Purity: min. 98% (HPLC)
MW = 523.2 g/mol (acid form)