Pisum sativum lectin (PSA)

Pisum sativum lectin or agglutinin (PSA) is isolated from peas and purified by affinity chromatography. The lectin has four subunits and a molecular weight of 49 kDa. PSA agglutinates human erythrocytes but is not blood group specific. It displays specificity toward α-D-mannose and α-D-glucose and has mitogenic activity similar to Concanavalin A.

Pisum sativum lectin is supplied as a white to cream coloured lyophilized powder. The purity of PSA is determined by SDS- electrophoresis, which generates two bands at 17 kDa and 5,7 kDa corresponding to the α- and ß-chains that are formed in solution, below pH5.8.

How to use
The lectin may be reconstituted with 2mL of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol.