FSL-1-Biotin - Pam2Cys-GDPKHPKSFK(Aca-Aca-Biotin)

FSL-1 (Pam2Cys-GDPKHPKSF) showed higher activity than MALP-2 when tested for its capability to activate THP-1 cells to produce TNF-alpha and on HEK293 cells transfected with TLR2 and TLR6 to produce NF-kappaB (Okusawa et al. 2004). It was shown that the activity of FSL-1 is TLR2 and TLR6 dependent. A single amino acid substitution from F to R (FSL-2, Pam2Cys-GDPKHPKSR, Cat-No- P2219) reduced the activity significantly (Okusawa et al. 2004). FSL-1 (P2212) is more favourable than MALP-2 with respect to solubility in aqueous solutions. FSL-1 is provided as stereochemically defined compounds and as mixture of stereoisomers. Lipopeptides with the S-dihydroxypropyl moiety are not active and therefore suitable as negative controls. Additionally, analogues with different labels or tags are available. Genaxxon offers further analogues differing in the peptide moiety for improved physico-chemical features and high reproducible biological activity. Please inquire for other peptides or more information: info@genaxxon.com.

FSL-1-Biotin is a selectively biotinylated analogue of FSL-1 TLR2/TLR6 Agonist (P2212). It is labelled via the side chain of an additional C-terminal lysine. Biotin has a strong affinity for avidin or streptavidin. This interaction can be used for detection, labelling or immobilisa-tion in many research applications. Lipopeptides are valuable tools for basic research in innate and acquired immunity. The synthetic lipopeptide FSL-1 represents the N-terminal sequence of the 44-kDa lipoprotein LP44 of Mycoplasma salivarium. It carries two ester bound fatty acids and a free amino terminus. The synthetic lipopeptide FSL-1 is described to elicit cellular responses through TLR2/TLR6 heterodimers which involves downstream NF-κB activation and cytokine release. The water soluble FSL-1 showed high activity when tested for its capability to activate THP-1 cells to produce TNF-α and on HEK293 cells transfected with TLR2 and TLR6 to produce NF-κB.