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Glutathione MagBeads are developed for the affinity purification of glutathione-S-transferase (GST) fusion proteins. The affinity matrix is based on spherical magnetic cross-linked agarose. The material is highly porous to allow optimal protein interaction. Cross-linked agarose is also physically very stable, making it suitable for purification processes without deformation or destruction. Our magnetic beads are very homogeneous in size with a medium particle diameter of 30µm, yielding a high degree of reproducibility between individual purification runs.
Glutathione is coupled to the magnetic agarose beads to obtain an affinity matrix with highest binding capacity for GST fusion proteins. Because the purification method depends on correctly folded GST protein, only native conditions can be used.
Glutathione MagBeads are delivered as a 25% suspension in 20% ethanol. Therefore, 1mL suspension will yield a 250µL bed volume. The suspension contains 20% ethanol to prevent microbial growth. Genaxxon MagBeads have a binding capacity of >10mg protein/mL settled beads.
Protein Binding Capacity
The protein binding capacity is up to 10mg/mL settled beads, as determined by purification of glutathione-S-transferase (GST) from E.coli cleared lysates, and quantified via spectrophotometry.
GST-tagged proteins expressed in bacteria, yeasts, insects and in cell culture supernatants can be readily purified in a single purification step even from proteins expressed at low levels. After binding of target molecules, the magnetic resin can be readily isolated with the aid of a magnet. The GST tag can be cleaved in bound condition or in eluted condition by specific proteases such as TEV-express. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. Genaxxon MagBeads are ferrimagnetic agarose beads coupled to glutathione, an efficient ligand for GST fusion proteins.
Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.
10mg/mL binding capacity
1mL of a 25% suspension of Glutathione Magnetic Beads in 20% ethanol.
Binding capacity: >10mg/mL
Genaxxon Glutathione MagBeads are stable in most commonly used aqueous buffers with a pH of 3-12 (e.g. 1M sodium acetate, pH4.0, or 6M guanidinium hydrochloride), organic solvents (e.g. 70% (v/v) ethanol), and 1% (w/v) SDS for 1h at room temperature.
Applikation:affinity purification of GST fusion proteins
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 32-16-04-02
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