Penicillin-Streptomycin (10000 U/mL)



Artikel-Nr.: M3140.0100

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Pen-Strep: Penicillin G aus Penicillium notatum und Streptomycin aus Streptomyces griseus... mehr
Produktinformationen "Penicillin-Streptomycin (10000 U/mL)"

Pen-Strep: Penicillin G aus Penicillium notatum und Streptomycin aus Streptomyces griseus verhindern mit ihrer kombinierten Wirkung (Pen-Strep) gegen grampositive und gramnegative Bakterien eine bakterielle Kontamination von Zellkulturen. Penicillin G wirkt einerseits direkt, indem es den Neuaufbau der bakteriellen Zellwand stört, sowie indirekt, indem es die Freisetzung von Enzymen auslöst, die die Zellwand weiter verändern. Streptomycin wirkt, indem es sich an die 30S-Untereinheit des bakteriellen Ribosoms anlagert, was zu einer Hemmung der Protein-Synthese und zum anschließenden Absterben der Bakterien führt.

Penicillin G Kaliumsalz ist in Wasser gut löslich. Bei +4°C oder Raumtemperatur ist die Trockensubstanz ca. 5 Jahre stabil. Die gepufferte Lösung (pH4,5-7) verliert bei +4°C nach 20 Tagen 5% an Aktivität und nach 60 Tagen ca. 20%. Bei Raumtemperatur ist bereits nach 48 Stunden ein 5%iger, nach 7 Tagen ein 10%iger und nach 14 Tagen ein 20 - 50%iger Aktivitätsverlust zu verzeichnen. In einer ungepufferten Lösung ist die Stabilität erheblich geringer (+4°C ca. 3-7 Tage).
Pen-Strep: Penicillin G und Streptomycin gegen bakterielle Kontamination von Zellkulturen.

Genaxxon bioscience bietet neben Pen-Strep eine breite Palette an Antibiotika und Antimykotika als Pulver und in flüssiger Form an, z.B. Geneticin G418, Kanamycin oder Gentamycinsulfat.

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• 10,000 mg/mL Streptomycin • 10 mg/mL Penicillin • 0.9% NaCl • effective against gram + and gram - bacteria • can be used prophylactic • Streptomycin-Penicillin is a broad band antibiotic • inhibits protein biosynthesis (streptomycin) • inhibits cell wall synthesis (penicillin)
Specifications: Penicillin G content: 9,000 – 11,000 U/mL Streptomycin content: 9 – 11 mg/mL... mehr
 

Technische Daten:

Specifications:
Penicillin G content: 9,000 – 11,000 U/mL
Streptomycin content: 9 – 11 mg/mL
0.9% NaCl (physiological NaCl solution)
pH: 6.0 – 7.0
Osmolality: 280 – 400 mOsmol/kg
Endotoxin: <0.1 EU/mL
Bioburden: 0 CFU/mL
Shelf-life at 37°C: 5 days

sterile filtered solution for cell culture applications

Applikation:

cell culture applications

Quelle

Steptomycin is isolated from Actinomycetes Streptomyces griseus

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 32-19-90-90
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Dokumente - Protokolle - Downloads

Hier finden Sie Informationen und weiterführende Literatur zu Penicillin-Streptomycin (10000 U/mL). Für weitere Dokumente (Zertifikate mit weiteren Lotnummern, Sicherheitsdatenblätter in anderer Sprache, weitere Produktinformationen) wenden Sie sich bitte an Genaxxon biosience unter: info@genaxxon.com oder Tel.: +49 731 3608 123.

 
 
 
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Äquivalent/Alternative zu: Biochrom Cat.-no. A 2213, oder zu Bio&Sell Nr. BS.A2213.

Change in colour of the Pen/Strep solution:
Normally this change in colour does not mean that you can not use the solution anymore. There are different reasons for it.
1. Chemical reaction of Penicillin and oxygen which will form a substance that absorbs light to get a pale yellow colour. But this does not mean that the majority of the Penicillin is degraded. THere is still enough antibiotic in the solution.
2. The other point is, sometimes, that the Streptomycin source does still contain some pigments from the strain from which Streptomycin is isolated. These pigments may also undergo a reaction with oxygen that turns them more yellowish.

In any case you feel not good about the yellowish colour you should discard it, at this will be the only possibility to have no uncertainty for explanations of further results.

 
 
 
Literatur .... mehr

Referenzen..

Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.

Quelle/Source: NCBI PubMed >

 

bFGF-mediated pluripotency maintenance in human induced pluripotent stem cells is associated with NRAS-MAPK signaling
Fereshteh Haghighi, Julia Dahlmann, Saeideh Nakhaei-Rad, Alexander Lang, Ingo Kutschka, Martin Zenker, George Kensah, Roland P. Piekorz, Mohammad Reza Ahmadian
Cell Commun Signal. 2018; 16: 96. Published online 2018 Dec 5. doi: 10.1186/s12964-018-0307-1
PMCID: PMC6282345

IL-23R Signaling Plays No Role in Myocardial Infarction
Erika Engelowski, Nastaran Fazel Modares, Simone Gorressen, Pascal Bouvain, Dominik Semmler, Christina Alter, Zhaoping Ding, Ulrich Flögel, Jürgen Schrader, Haifeng Xu, Philipp A. Lang, Jens Fischer, Doreen M. Floss, Jürgen Scheller
Sci Rep. 2018; 8: 17078. Published online 2018 Nov 20. doi: 10.1038/s41598-018-35188-8
PMCID: PMC6244091

Prevention of bacterial colonization on non-thermal atmospheric plasma treated surgical sutures for control and prevention of surgical site infections

Utku Kürşat Ercan, Fatma İbiş, Caner Dikyol, Nesrin Horzum, Ozan Karaman, Çağla Yıldırım, Elif Çukur, Emine Afra Demirci

PLoS One. 2018; 13(9): e0202703. Published online 2018 Sep 5. doi: 10.1371/journal.pone.0202703

PMCID: PMC6124751

 

Synthetic cytokine receptors transmit biological signals using artificial ligands

Erika Engelowski, Artur Schneider, Manuel Franke, Haifeng Xu, Ramona Clemen, Alexander Lang, Paul Baran, Christian Binsch, Birgit Knebel, Hadi Al-Hasani, Jens M. Moll, Doreen M. Floß, Philipp A. Lang, Jürgen Scheller

Nat Commun. 2018; 9: 2034. Published online 2018 May 23. doi: 10.1038/s41467-018-04454-8

PMCID: PMC5964073

 

IL-6/IL-12 Cytokine Receptor Shuffling of Extra- and Intracellular Domains Reveals Canonical STAT Activation via Synthetic IL-35 and IL-39 Signaling

D. M. Floss, M. Schönberg, M. Franke, F. C. Horstmeier, E. Engelowski, A. Schneider, E. M. Rosenfeldt, J. Scheller

Sci Rep. 2017; 7: 15172. Published online 2017 Nov 9. doi: 10.1038/s41598-017-15173-3

PMCID: PMC5680241

 

Synthetic Deletion of the Interleukin 23 Receptor (IL-23R) Stalk Region Led to Autonomous IL-23R Homodimerization and Activation

Thorben M. Hummel, Theresa Ackfeld, Marco Schönberg, Gregor Ciupka, Falk Schulz, Anne Oberdoerster, Joachim Grötzinger, Jürgen Scheller, Doreen M. Floss

Mol Cell Biol. 2017 Sep 1; 37(17): e00014-17. Prepublished online 2017 Jun 19. Published online 2017 Aug 11. doi: 10.1128/MCB.00014-17

PMCID: PMC5559674

 

Defining the functional binding sites of interleukin 12 receptor β1 and interleukin 23 receptor to Janus kinases

Doreen M. Floss, Tobias Klöcker, Jutta Schröder, Larissa Lamertz, Simone Mrotzek, Birgit Strobl, Heike Hermanns, Jürgen Scheller

Mol Biol Cell. 2016 Jul 15; 27(14): 2301–2316.  doi: 10.1091/mbc.E14-12-1645

PMCID: PMC4945146

 

A widespread sequence-specific mRNA decay pathway mediated by hnRNPs A1 and A2/B1

Rene Geissler, Alfred Simkin, Doreen Floss, Ravi Patel, Elizabeth A. Fogarty, Jürgen Scheller, Andrew Grimson

Genes Dev. 2016 May 1; 30(9): 1070–1085.  doi: 10.1101/gad.277392.116

PMCID: PMC4863738

 

MicroRNA-34a promotes genomic instability by a broad suppression of genome maintenance mechanisms downstream of the oncogene KSHV-vGPCR

Claudia J. Krause, Oliver Popp, Nanthakumar Thirunarayanan, Gunnar Dittmar, Martin Lipp, Gerd Müller

Oncotarget. 2016 Mar 1; 7(9): 10414–10432.  Published online 2016 Feb 8. doi: 10.18632/oncotarget.7248

PMCID: PMC4891129

 

 

Sertoli cells have a functional NALP3 inflammasome that can modulate autophagy and cytokine production

Soren Hayrabedyan, Krassimira Todorova, Asma Jabeen, Gergana Metodieva, Stavri Toshkov, Metodi V. Metodiev, Milcho Mincheff, Nelson Fernández

Sci Rep. 2016; 6: 18896.  Published online 2016 Jan 8. doi: 10.1038/srep18896

PMCID: PMC4705529

 

Sec14-nodulin proteins and the patterning of phosphoinositide landmarks for developmental control of membrane morphogenesis

Ratna Ghosh, Marília K. F. de Campos, Jin Huang, Seong K. Huh, Adam Orlowski, Yuan Yang, Ashutosh Tripathi, Aaron Nile, Hsin-Chieh Lee, Marek Dynowski, Helen Schäfer, Tomasz Róg, Marta G. Lete, Hasna Ahyayauch, Alicia Alonso, Ilpo Vattulainen, Tatyana I. Igumenova, Gabriel Schaaf, Vytas A. Bankaitis

Mol Biol Cell. 2015 May 1; 26(9): 1764–1781.  doi: 10.1091/mbc.E14-10-1475

PMCID: PMC4436786

 

Non-Canonical Interleukin 23 Receptor Complex Assembly: P40 PROTEIN RECRUITS INTERLEUKIN 12 RECEPTOR β1 VIA SITE II AND INDUCES P19/INTERLEUKIN 23 RECEPTOR INTERACTION VIA SITE III

Jutta Schröder, Jens M. Moll, Paul Baran, Joachim Grötzinger, Jürgen Scheller, Doreen M. Floss

J Biol Chem. 2015 Jan 2; 290(1): 359–370.  Published online 2014 Nov 4. doi: 10.1074/jbc.M114.617597

PMCID: PMC4281739

 

Alternative Intronic Polyadenylation Generates the Interleukin-6 Trans-signaling Inhibitor sgp130-E10

Jan Sommer, Christoph Garbers, Janina Wolf, Ahmad Trad, Jens M. Moll, Markus Sack, Rainer Fischer, Joachim Grötzinger, Georg H. Waetzig, Doreen M. Floss, Jürgen Scheller

J Biol Chem. 2014 Aug 8; 289(32): 22140–22150.  Published online 2014 Jun 27. doi: 10.1074/jbc.M114.560938

PMCID: PMC4139227

 

Identification of autophagy as a longevity-assurance mechanism in the aging model Podospora anserina

Laura Knuppertz, Andrea Hamann, Francesco Pampaloni, Ernst Stelzer, Heinz D Osiewacz

Autophagy. 2014 May; 10(5): 822–834.  Published online 2014 Feb 27. doi: 10.4161/auto.28148

PMCID: PMC5119060

 

PKCβ Phosphorylates PI3Kγ to Activate It and Release It from GPCR Control

Romy Walser, John E. Burke, Elena Gogvadze, Thomas Bohnacker, Xuxiao Zhang, Daniel Hess, Peter Küenzi, Michael Leitges, Emilio Hirsch, Roger L. Williams, Muriel Laffargue, Matthias P. Wymann

PLoS Biol. 2013 Jun; 11(6): e1001587.  Published online 2013 Jun 25. doi: 10.1371/journal.pbio.1001587

PMCID: PMC3692425

 

Identification of Canonical Tyrosine-dependent and Non-canonical Tyrosine-independent STAT3 Activation Sites in the Intracellular Domain of the Interleukin 23 Receptor

Doreen M. Floss, Simone Mrotzek, Tobias Klöcker, Jutta Schröder, Joachim Grötzinger, Stefan Rose-John, Jürgen Scheller

J Biol Chem. 2013 Jul 5; 288(27): 19386–19400.  Published online 2013 May 14. doi: 10.1074/jbc.M112.432153

PMCID: PMC3707643

 

Mutational analysis of a phenazine biosynthetic gene cluster in Streptomyces anulatus 9663

Orwah Saleh, Katrin Flinspach, Lucia Westrich, Andreas Kulik, Bertolt Gust, Hans-Peter Fiedler, Lutz Heide

Beilstein J Org Chem. 2012; 8: 501–513.  Published online 2012 Apr 4. doi: 10.3762/bjoc.8.57

PMCID: PMC3326630

 

The biosynthetic genes for prenylated phenazines are located at two different chromosomal loci of Streptomyces cinnamonensis DSM 1042

Kerstin Seeger, Katrin Flinspach, Elisa Haug‐Schifferdecker, Andreas Kulik, Bertolt Gust, Hans‐Peter Fiedler, Lutz Heide

Microb Biotechnol. 2011 Mar; 4(2): 252–262.  Published online 2011 Feb 22. doi: 10.1111/j.1751-7915.2010.00234.x

PMCID: PMC3818865

 

Aromatic Prenylation in Phenazine Biosynthesis: DIHYDROPHENAZINE-1-CARBOXYLATE DIMETHYLALLYLTRANSFERASE FROM STREPTOMYCES ANULATUS

Orwah Saleh, Bertolt Gust, Björn Boll, Hans-Peter Fiedler, Lutz Heide

J Biol Chem. 2009 May 22; 284(21): 14439–14447.  doi: 10.1074/jbc.M901312200

PMCID: PMC2682892