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Tris base of highest quality. Purity: min. 99.9%. Useful for buffers of pH7.2 to 9.0. For use in biochemistry and enzymatic research.
Tris is the most commonly used buffer in biological research. One of its most important applications is the use as an electrophoresis buffer (e.g. TBE (M3206 Buffer Grade 10-time concentrated, M3088 MolBio Grade 10-time concentrated, M3405 Buffer Grade 5-time concentrated) and D2033 (ready-to-use powder) or TAE (M3085 10-times concentrated and M3087 50-times concentrated) for polyacrylamide and agarose gel electrophoresis, respectively. Besides, Tris is used as TE buffer (pH8.0) for the storage and dissolving of nucleic acids. EDTA protects the nucleic acids by complexing Me++-ions needed from nucleases for their activity.
A 1M Tris solution is made up by dissolving 121g Tris base in 800mL of dionized water, adjusting to the desired pH with conc. HCl and filling up to 1 Liter with deionized water. Tris should not be used at pH values under ~pH 7.2 or above ~pH 9.0. The pH value of a Tris buffer strongly depends on the temperature and the pH changes by 0.1 units when diluted by a factor of 10. Therfore, Tris buffers should be prepared at the temperature where it is used and the pH changes should be taken into consideration when the buffer should be diluted.
Tris will inactivate the RNase inhibitor DEPC, which is commonly used to treat equipment and solutions, that get into contact with the RNA during its preparation.
Synonym: THAM, Tris-(hydroxymethyl)-aminomethane, Tris-Base, Trometamol, Tromethamine, Amino-2-(hydroxymethyl)-propan-1,3-diol.
Application and Literature
1. Blocking in western blots by incubation of the nitrocellulose membrane after blotting with TBST (10 mM Tris-HCl, pH8.0, 150mM NaCl, 0.05% Tween® 20) with 5% nonfat dried milk (Takemoto, Y. et al. (1995) EMBO J. 14, 3403-3414) or TBS (100mM Tris-HCl, pH7.5, 0.9% NaCl) with 10% (w/v) nonfat dried milk (Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology. Supplement 39 Page 10.8.10; Greene Publishing & Wiley-Interscience, New York).
2. TBE is one of the most commonly used electrophoresis buffers with the composition for a 10X buffer: 890mM Tris base, 890mM boric acid, 20mM EDTA (pH8.3) (for 1Liter: 108g Tris base, 9.3g EDTA x Na2, 55g boric acid, pH8.3). (Peacock, A.C. & Dingman, C.W. (1967) Biochemistry 6, 1818-1827; Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology. Supplement 40 Page A.2.5, Greene Publishing & Wiley-Interscience, New York; Ogden, R.C. & Adams, D.A. (1987) Methods Enzymol. 152, 61-87).
Purity (titr.): min. 99.9%
pH (10%, H2O, 20°C): 10.5-11.5
Solubtility (H2O): 800 g/L (clear, colourless
pKa (20°C): 8.3
Water: max. 0.2%.
Heavy metals (as Pb): max. 0.0001%
MW = 121.14 g/mol
Sicherheits Hinweise / SafetyH-Sätze: H315, H319
Klassifizierungen / ClassificationEC-Nr: 201-064-4
Dokumente - Protokolle - Downloads
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