CPM - 7-Diethylamino-3-(4´-maleimidylphenyl)-4-methylcoumarin

Order number: S5508.0050

Shipping: shipped at RT, stored at -20°C. Protect from light and moisture.
CAS-Nr: 76877-33-3

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CPM is a widely used blue fluorescent thiol-reactive dye (Ex/Em: 384/470nm). This maleimide... more

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Product information "CPM - 7-Diethylamino-3-(4´-maleimidylphenyl)-4-methylcoumarin"

CPM is a widely used blue fluorescent thiol-reactive dye (Ex/Em: 384/470nm). This maleimide derivative of coumarin is essentially non-fluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step. Good energy acceptor from tryptophan and a good energy donor to fluorescein. Used to monitor release of thiols and to distinguish proliferating cancer cells by nucleolar protein staining.

References:
C.C. Chung; Assay and Drug Devel. Techn. 6(3), 361 (2008)
O. Khersonsky et al.; ChemBioChem 7(1), 49 (2006)
J.E.T. Corrie et al.; J. Chem. Soc. Trans. Perkin Trans. 1, 2975 (1994)

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Our comment on "CPM - 7-Diethylamino-3-(4´-maleimidylphenyl)-4-methylcoumarin"

Good energy acceptor from tryptophan and a good energy donor to fluorescein. Ex/Em: 384/470nm

Assay: >95% (HPLC); Identity: H-NMR; Appearance: yellow powder; C24H22N2O4; MW = 402.44... more

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Technical Data:

Assay: >95% (HPLC); Identity: H-NMR; Appearance: yellow powder; C24H22N2O4; MW = 402.44 g/mol.
Soluble in DMSO, DMF, acetonitrile or chloroform.

application:

Used to monitor release of thiols and to distinguish proliferating cancer cells by nucleolar protein staining.

Source

synthetic

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

CAS-Nr: 76877-33-3
eclass-Nr: 34-16-04-90

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