5-TAMRA-DMTr-phosphoramidite
Order number: S5432.0100
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5-Carboxytetramethylrhodamine DMTr-CE-phosphoramidite (TAMRA-phosphoramidite) for automated 5'- and 3'-labeling of oligonucleotides (Abs: 549 nm, Em: 572 nm). Single isomer TAMRA phosphoramidite derivatives are used as highly convenient red fluorescent derivatization reagents in automated nucleic acid labeling protocols.
The fluorescence quantum yield of TAMRA conjugates is about one-fourth that of fluorescein conjugates. Nevertheless, TAMRA conjugates often appear brighter than the corresponding fluorescein conjugates due to the fact that TAMRA is readily excited by the 546 nm spectral line from mercury-arc lamps used in most fluorescence microscopes and is intrinsically more photostable than fluorescein. TAMRA is also efficiently excited by the 543 nm spectral line of the green He-Ne laser.
Not a hazardous substance or mixture according to GHS [Regulation (EC) No. 1272/2008]. This substance or mixture is not classified as dangerous according to Directive 67/548/EEC. The product does not need to be labelled in accordance with EC directives or
Technical Data:
Absorption: 549 nm (ε = 63.000 M-1cm-1); Emission: 572 nm.
