- Biochemikalien - weitere Produkte

AEBSF is an irreversible inhibitor of Thrombin and other Serin proteases (Chymotrypsin, Kallikrein, Plasmin, Proteinase K, Trypsin) by sulfonylation of a functional group in the active centre of the enzyme. AEBSF is much less toxic compared to PMSF and DFP and therefore recommended to substitute these reagents. AEBSF is highly soluble in water resulting in a solution of pH 6.5. At this pH AEBSF is most stable. We recommend to store stock solutions (20 mM or 100 mM in water or buffer) at -20°C for up to 2 months and prepare fresh working solutions. AEBSF is used at a working concentration of 0.1 - 0.2 mM. Walsmann, P. et al. (1972) Acta biol. med. germ. 28, 577-585, Nathan, D.F. & Linquist, S. (1995) Mol. Cell. Biol., 15, 3917-3925. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Description:Aprotinin is mainly obtained from bovine lung. Aprotinin consists of 58 amino acids of 6511 Da and is very basic, whereby amino acids 13-18 of Aprotinin are of particular importance for binding to serine proteases. As Aprotinin has been described by various research groups, two names have become established: 1. bovine pancreatic trypsin inhibitor (BPTI) and 2. trypsin-kallikrein inhibitor (TKI). Aprotinin is often used as a non-specific serine protease inhibitor, especially for inhibition of Trypsin. Effect:Aprotinin inhibits proteases such as Trypsin, Plasmin, Chymotrypsin and Thrombin. Aprotinin is a competitive inhibitor of serine proteases that forms stable complexes with the enzyme and blocks active enzyme sites. These bindings are reversible and most aprotinin-protease complexes dissolve at very low or high pH values (<3 and >10).Application:Aprotinin is mainly used as a trypsin inhibitor. Stability: Aprotinin can be stored in lyophylised form at +2°C to +8°C for an unlimited period of time. In salt or buffer solutions (pH 5 - 8), Aprotinin is stable for at least one month at +2°C to +8°C or for several years at -20°C. Stock solutions (e.g. 10mg/mL) should be frozen in small aliquots and used only once to avoid repeated freezing and thawing. Unit definition:One trypsin inhibitory unit (TIU) inhibits the activity of 2 trypsin units by 50%, whereby one trypsin unit hydrolyses 1 μmol Nα-benzoyl-DL-arginine-p-nitroanilide (BAPNA) per minute at pH 7.8 and 25°C. 1 TIU (trypsin inhibitory unit) corresponds to approx. 1300 KIU (kallikrein inhibitory units) and 1 TIU corresponds to one Ph. Eur. unit.

TPCK is an irreversible, specific inhibotor for chymotrypsin and inhibits the thrombin-like protease Cerastocytin. Trypsin is not inhibited by TPCK. The pH optimum for inhibition throµgh alkylation by TPCK of Chymotrypsin is pH7.2. Chymotrypsinogen is not inhibited by TPCK. The bacterial Serin-proteases Re, Fa and So from E.coli are completely inhibited by 0.5 to 1 mM TPCK. TPCK is used for the production of Chymotrypsin free Trypsin. TPCK solutions are stable at pH below 7.0 at RT, nevertheless it is recommended to store solutions at +2°C to +8°C. TPCK is not stable at pH above 7.0. Stock solutions of 3mg/mL to 20mg/mL are prepared in MeOH or EtOH. Working concentrations: 10µg/mL to 100µg/mL. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Adenosine triphosphate (ATP) is the universal and immediately available energy source in cells and an important regulator of energy providing processes. The molecule adenosine triphosphate consists of an adenine, the sugar ribose and three phosphate (α to γ) in ester (α) or anhydride bond (β and γ). It was shown that ATP has an inhibitory effect on the inflammatory response by abrogating secretion of TNF-α and promoting secretion of Interleukin-10.  Synonyms: ATP, Adenosin-5'-Triphosphat.  Also available from Genaxxon: NADH Na salt (reduced) >.

Synonym: Adenosin, Adenosine, 9-β-D-Ribofuranosyladenine, Adenine riboside, Adenine-9-β-D-ribofuranoside, D-Adenosine

L-Methionine sulfoximine is a potent inhibitor of glutamine sythetase activity, that is widely used as a selection agent for plasmid integration in Chinese hamster ovary (CHO) and other mammalian cell lines. The growing demand for high yield cell banks for production of recombinant proteins for therapeutics has resulted in two major systems for selection of stable and active clones, Methotrexate selection of dihydrofolate reductase (DHFR) overexpressing cells and MSX selection of glutamine synthetase overexpressing cells. Cells are grown in the absence of glutamine in the media and inhibition of the endogenous activity of glutamine synthease results in cell death for cells lacking overexpression. The MSX-glutamine synthase selection mechanism provides benefits over that of the Methotrexate-DHFR system in that it typically requires a single amplification step and results in significant reduction of time to produce high stability, highly amplified clones. Methionine sulfoximine (MSX) increases ornithine decarboxylase activity, decreases the survival rate in a model of transient cerebral ischemia, and inhibits glutamine synthetase activity. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Mixture of 6 protease inhibitors with a broad range activity against aspartate-, cysteine-, serine-, and metallo proteases as well as aminopeptidases. It is especially formulated for use with extracts from mammalian tissue, but can also be used with other extracts. This ready-to-use mix is free of EDTA and contains AEBSF >, Aprotinin >, E-64 >, Leupeptin >, Bestatine >, and Pepstatin A >. The kit is supplied with an extra vial of 1mL ultrapure DMSO. The content of 1 vial dissolved in 1mL DMSO will give a 100-fold concentrate, suitable for the treatment of 100mL extract. Activity spectrumAEBSF is an inhibitor against Serine proteases. Aprotinin is an inhibitor against Serine proteases. E-64 is an inhibitor against Cysteine proteases. Leupeptin is an inhibitor against Cystein proteases and trypsin-like Serine proteases. Peptstatin A is an inhibitor against Aspartic proteases. Bestatin is an inhibitor against Aminopeptidase B and Leucine-Aminopeptidases. More ready-to-use protease inhibitor mixes can be found here: - Protease-Inhibitor-Mix I > (general applications, contains no EDTA) - Protease-Inhibitor-Mix II > (general applications, contains EDTA)- Protease-Inhibitor-Mix III > (formulated for use with bacterial extracts, contains EDTA)- Protease-Inhibitor-Mix IV > formulated for use with plant extracts, contains no EDTA)- Protease-Inhibitor-Mix V > (formulated for use with mammalian extracts, contains no EDTA) You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

The Protease-Inhibitor-Mix IV for plant extracts is a mixture of 6 protease inhibitors with a broad range activity against aspartate-, cysteine-, serine-, and metallo proteases as well as aminopeptidases. It is especially formulated for use with plant extracts. This ready-to-use mix contains AEBSF >, E-64 >, Leupeptin >, Bestatin >, Pepstatin A > and 1,10-Phenanthroline. The kit is supplied with an extra vial of 1mL ultrapure DMSO. The content of 1 vial dissolved in 1mL DMSO will give a 100-fold concentrate, suitable for the treatment of 100mL extract. More ready-to-use protease inhibitor mixes can be found here: - Protease-Inhibitor-Mix I > (general applications, contains no EDTA) - Protease-Inhibitor-Mix II > (general applications, contains EDTA)- Protease-Inhibitor-Mix III > (formulated for use with bacterial extracts, contains EDTA)- Protease-Inhibitor-Mix IV > formulated for use with plant extracts, contains no EDTA)- Protease-Inhibitor-Mix V > (formulated for use with mammalian extracts, contains no EDTA) You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Protease-Inhibitor-Mix III for bacterial extracts is mixture of 5 protease inhibitors with a broad range activity against aspartate-, cysteine-, serine-, and metallo proteases as well as aminopeptidases. It is especially formulated for use with bacterial extracts. This ready-to-use mix contains AEBSF >, E-64 >, Pepstatin A >, Bestatine > and EDTA. The kit is supplied with an extra vial of 1mL ultrapure DMSO. The content of the inhibitor mix dissolved in 1mL DMSO lasts for the treatment of 100mL bacterial extract / bacterial lysate. The content of 1 vial dissolved in 1mL DMSO will give a 100-fold concentrate, suitable for the treatment of 100mL bacterial extract / bacterial lysate. Activity spectrumAEBSF is an inhibitor against Serine proteases. E-64 is an inhibitor against Cysteine proteases. Peptstatin A is an inhibitor against Aspartic proteases. Bestatin is an inhibitor against Aminopeptidase B and Leucine-Aminopeptidases and EDTA inhibits Metallo proteases. More ready-to-use protease inhibitor mixes can be found here: - Protease-Inhibitor-Mix I > (general applications, contains no EDTA) - Protease-Inhibitor-Mix II > (general applications, contains EDTA)- Protease-Inhibitor-Mix III > (formulated for use with bacterial extracts, contains EDTA)- Protease-Inhibitor-Mix IV > formulated for use with plant extracts, contains no EDTA)- Protease-Inhibitor-Mix V > (formulated for use with mammalian extracts, contains no EDTA) You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

The Protease-Inhibitor-Mix I for recombinant protein is a special mixture of 4 water-soluble protease inhibitors with broad specificity for the inhibition of cysteine-, serine- and metalloproteases. Recommended for general applications and where the use of organic solvents should be avoided. This mix contains AEBSF >, Aprotinin >, E-64 >, Leupeptin > but no EDTA! The content of 1 vial dissolved in 1mL water results in a 100-fold concentrate suitable for the treatment of 100mL tissue extract. More ready-to-use protease inhibitor mixes can be found here: - Protease-Inhibitor-Mix I > (general applications, contains no EDTA) - Protease-Inhibitor-Mix II > (general applications, contains EDTA)- Protease-Inhibitor-Mix III > (formulated for use with bacterial extracts, contains EDTA)- Protease-Inhibitor-Mix IV > formulated for use with plant extracts, contains no EDTA)- Protease-Inhibitor-Mix V > (formulated for use with mammalian extracts, contains no EDTA) You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Colcemid inhibits the formation of mitotic spindles. It is used to increase the percentage of metaphase cells for chromosome analysis. Colcemid depolymerizes microtubules; blocks mitosis at metaphase. Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

E-64 is an irreversible inhibitor of different cysteine proteases (papain, bromelain, ficin, cathepsin B, H and L, tumor-cathepsin, calpain, V-CATH from Autographa californica M nucleopolyhedro virus (AcMNPV) and Streptococcus-protease). All enzymes are completely inhibited by a concentration of 10 - 30 μM within 30 minutes. E-64 and other protease inhibitors (e. g. diisopropylfluorophosphate and PMSF) are able to inhibit the programmed cell death, that is induced by activation of the T cell receptor in different cell lines of the immune system, not by blocking the signal transduction. The effect of dexamethason, which induces apoptosis as an antagonist of the T cell receptor, is enhanced. If proteins are expressed in the baculo virus system with a functional v-cath gene, very often a proteolytic activity can be observed. This reduces the quality of the expressed protein significantly. The addition of E-64 to all reaction steps avoids the degradation almost completely. Stability: E-64 is stable against reducing agents. Stock solutions have concentrations between 1mM and 10mM in DMSO or 50% ethanol and are stable for several days, if stored at +4°C. Aliquots may be stored at -20°C for approx. one month. Synonyme: trans-Epoxysuccinyl-L-leucylamido(4-guanidino)butan; L-trans-3-Carboxyoxiran-2-carbonyl-L-leucylagmatin; N-(trans-Epoxysuccinyl)-L-leucin-4-guanidinobutylamid You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Bestatin is a potent aminopeptidase inhibitor. The compound has multiple physiological functions including the ability to act as an immunomodifier and enhance the proliferation of human bone marrow granulocyte-macrophage progenitor cells to form CFU-GM colonies. Research indicates that bestatin can indirectly act on monocytes by inhibiting Aminopeptidase B, which in turn inhibits the catabolism of tuftsin. Bestatin has also been shown to inhibit Leukotriene A4 hydrolase (LTA4H). This compound has been demonstrated to bind to cell surfaces and research indicates that it can directly stimulate lymphocytes through its fixation on the cell surface. The agent has been used in multiple leukemia studies. Synonym: N-[(2S,3S)-3-Amino-2-hydroxy-4-phenyl-butyryl]-L-Leucine hydrochloride. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

TLCK is an irreversible, specific inhibotor of the Serin-protease Trypsin and inhibits the Thrombin-like protease Cerastocytin. Together with the competitive inhibitors benzamidin hydrochlorid or 8M urea Trypsin does not show inhibitiory effects. Chymotrypsin is not inhibited by TLCK. The pH optimum for inhibition through Histidin modification is pH 7.5. Chymotrypsinogen is not inhibited by TLCK. A concentration of 50 µg/mL of TLCK inhibits 100% of the Trypsin like proteinase activity of wheat seat extracts. Besides proteases also protein kinases are inhibited by TLCK. Obviously by the modification of the enzymatic activity centre of the protein kinases. TLCK solutions are most stable at pH 6.0 at +4°C. TLCK is not stable at pH above 7.0. Therefore stock solutions of 1 mg/mL to 20 mg/mL are prepared with buffer solutions of pH below 6.0. Always pipete shortly before the inhibition experiment. Working solutionsa are: 50 µg/mL to 100 µg/mL. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

a-D-Glucose-1-phosphate dipotassium salt dihydrate, Cori-Ester di-K (synthetic crystalline product). a-D-Glucopyranose-phosphate. Free carbonate and phosphate are completely removed. Product shipped with certificate covering all data for validation (NMR, FTIR, GC, GC-MS, AAS, HPLC, optical rotation and MP. Glc-1-P.

Calcium lactate pentahydrate - Lactic acid calcium salt Synonym: L-Lactic acid calcium salt; Calcium L-lactate pentahydrate; Calcium L-lactate pentahydrate; Calcii lactas pentahydricus; L-Lactic acid calcium salt; Calcium lactate hydrate; Calcium L-lactate hydrate; L-Lactic acid calcium salt hydrate

a-D-Galactose-1-phosphate dipotassium salt dihydrate (synthetic crystalline product). Free carbonate and phosphate are completely removed. a-D-Galactose-1-phosphate. Product shipped with certificate covering all data for validation (NMR, FTIR, GC, GC-MS, AAS, HPLC, optical rotation and MP. Gal-1-P.

Protease inhibitor mix. Special mixture of 5 water-soluble protease inhibitors with broad specificity for the inhibition of cysteine-, serine- and metalloproteases. Recommended for general applications and where the use of organic solvents should be avoided. This mix contains AEBSF >, Aprotinin >, E-64 >, Leupeptin > and EDTA. The content of 1 vial dissolved in 1mL water results in a 100-fold concentrate suitable for the treatment of 100mL crude protein extract. Activity spectrumAEBSF is an inhibitor against Serine proteases. Aprotinin is an inhibitor against Serine proteases. E-64 is an inhibitor against Cysteine proteases. Leupeptin is an inhibitor against Cystein proteases and trypsin-like Serine proteases and EDTA as an inhibitor against Metallo proteases. More ready-to-use protease inhibitor mixes can be found here: - Protease-Inhibitor-Mix I > (general applications, contains no EDTA) - Protease-Inhibitor-Mix II > (general applications, contains EDTA)- Protease-Inhibitor-Mix III > (formulated for use with bacterial extracts, contains EDTA)- Protease-Inhibitor-Mix IV > formulated for use with plant extracts, contains no EDTA)- Protease-Inhibitor-Mix V > (formulated for use with mammalian extracts, contains no EDTA) You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

a-D-Mannose-1-phosphate dipotassium salt dihydrate (synthetic crystalline product). a-D-Mannopyranose-phosphate. Free carbonate and phosphate are completely removed. Product shipped with certificate covering all data for validation (NMR, FTIR, GC, GC-MS, AAS, HPLC, optical rotation and MP. Man-1-P.

Diethylpyrocarbonate modifies histidyl residues in proteins and leads to their inactivation. In molecular biology it is mainly used as a strong inhibitor of RNase activity. In addition, DEPC reacts with adenosine of single stranded nucleic acids. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Leupeptin works as peptide antibiotic that has been isolated from different Streptomyces species. It is a reversible inhibitor of serine and cysteine proteases, eg. Plasmin, Trypsin, Papain, Cathepsin B and Thrombin. It does not inhibit pepsin, cathepsin A and D. The working concentration is 10-100µM (5 - 50µg/mL). Leupeptin is soluble in water. A 1mg/mL stock solution can be stored at -20°C for 6 months. Umezawa, H. (1976) Methods Enzymol. 45 (Part B), 678-695. Brass, L.F. & Smith, S.J. (1988) J. Biol. Chem. 263, 5210-5216. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Isolated from Streptomyces testaceus. Potent inhibitor of acid proteases (aspartic proteases), including pepsin, renin and cathepsin D and many microbial aspartic proteases. Effective concentration 1 µM. Stable for about 1 day to 1 week at +4°C. Stock solutions (1 mM) are stable for several months at -20°C. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Aphidicolin inhibits the growth of eukaryotic cells and certain animal viruses by selectively inhibiting the cellular replication of DNA polymerase II or the viral-induced DNA polymerases. The drug may be useful for controlling excessive cell proliferation in patients with cancer, psoriasis or other dermatitis with little or no adverse effect upon non-multiplying cells. Aphidicolin is a tool in cell proliferation and differentiation research. Aphidicolin may possibly be used for controlling excessive cell proliferation in cancer, psoriasis or other dermatitis with little or no adverse effect upon non-multiplying cells. Synonyms:(3-a,4-a,5-,17-a)-3,17-Dihydroxy-4-methyl-9,15-cyclo-C,18-dinor14,15-secoandrostane-4,17-dimethanole. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Etoposide phosphate (Eposin®, Etopophos®, Vepesid®, VP-16®) is an inhibitor of the enzyme topoisomerase II. For medical purposes it is used as a form of chemotherapy for malignancies such as lung cancer, testicular cancer, lymphoma, non-lymphocytic leukemia, and glioblastoma multiforme. It is often given in combination with other drugs. Chemically it derives from podophyllotoxin, a toxin found in the American Mayapple. Genaxxon offers this product only for in-vitro (laboratory) usage! You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Citric acid trisodium salt (tri-sodium citrate, sodium citrate) is used as a buffering substance for molecular biology buffers.

Urea powder preweight and packed in aluminium foil. ready-to-use for preparing 100mL of a 5M urea solution.

1 tablet dissolved in 100mL of deionized water yields: 0.9% sodium chloride. These tablets are prepared from pharmaceutical grade reagents. Sodium chloride is widely used in many laboratory routines. For greater convenience a blister pack with 10 tablets is also avaliable. The reagent is 99.99% pure.

ready-to-use sodium chloride tablets. 1 tablet dissolved in 1000mL of deionized water yields: 0.9% sodium chloride. These tablets are prepared from pharmaceutical grade reagents. Sodium chloride is widely used in many laboratory routines. For greater convenience a blister pack with 10 tablets is also avaliable. The purity of the used Sodium chloride is >99.9%.

Content of 1 pouch dissolved in deionized water and made up to 1000mL yields a 3M Sodium chloride solution. No weighing necessary.

The Prestained Protein Marker - 10-245 kDa is a three-color protein standard with 12 pre-stained (covalently coupled to a chromophore) purified proteins. Proteins are covalently coupled with a blue chromophore except for two reference bands, one green at 25 kDa and one red one at 75 kDa respectively, when separated on SDS-PAGE (Tris-glycine buffer). The protein bands represent 10 kDa, 15 kDa, 20 kDa, 25 kDa (green), 35 kDa, 45 kDa, 60 kDa, 75 kDa (red), 100 kDa, 140 kDa, 180 kDa and 245 kDa. The Prestained Protein Marker 10-245 kDa is delivered in convenient 2 x 250µL aliquots. With a recommended application load of 3-5μL per lane, the marker lasts for up to 100 gels.

The Prestained Protein Marker - 10-245 kDa is a three-color protein standard with 12 pre-stained (covalently coupled to a chromophore) purified proteins. Proteins are covalently coupled with a blue chromophore except for two reference bands, one green at 25 kDa and one red one at 75 kDa respectively, when separated on SDS-PAGE (Tris-glycine buffer). The protein bands represent 10 kDa, 15 kDa, 25 kDa (green), 35 kDa, 45 kDa, 60 kDa, 75 kDa (red), 100 kDa, 140 kDa, and 180 kDa. The Prestained Protein Marker 10-245 kDa is delivered in convenient 2 x 250µL aliquots. With a recommended application load of 3-5μL per lane, the marker lasts for up to 100 gels.